Analysis of Branched-Chain Keto Acids in Cell Extracts by HPLC-Fluorescence Detection

Hattori, Ayuna; Ito, Takahiro; Tsunoda, Makoto

doi:10.15583/jpchrom.2017.010

Cited by 7 publications

(2 citation statements)

References 19 publications

(27 reference statements)

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“…Quantification entailing OPD derivatization and fluorescence detection has been reported by several groups, wherein LODs were between 18 nM and 1 µM [58][59][60]. DMB was developed to improve the sensitivity of fluorescence detection, and has a structure similar to that of OPD.…”

Section: α-Keto Acids and Their Metabolitesmentioning

confidence: 99%

Liquid-Chromatographic Methods for Carboxylic Acids in Biological Samples

et al. 2020

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Carboxyl-bearing low-molecular-weight compounds such as keto acids, fatty acids, and other organic acids are involved in a myriad of metabolic pathways owing to their high polarity and solubility in biological fluids. Various disease areas such as cancer, myeloid leukemia, heart disease, liver disease, and lifestyle diseases (obesity and diabetes) were found to be related to certain metabolic pathways and changes in the concentrations of the compounds involved in those pathways. Therefore, the quantification of such compounds provides useful information pertaining to diagnosis, pathological conditions, and disease mechanisms, spurring the development of numerous analytical methods for this purpose. This review article addresses analytical methods for the quantification of carboxylic acids, which were classified into fatty acids, tricarboxylic acid cycle and glycolysis-related compounds, amino acid metabolites, perfluorinated carboxylic acids, α-keto acids and their metabolites, thiazole-containing carboxylic acids, and miscellaneous, in biological samples from 2000 to date. Methods involving liquid chromatography coupled with ultraviolet, fluorescence, mass spectrometry, and electrochemical detection were summarized.

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Section: α-Keto Acids and Their Metabolitesmentioning

confidence: 99%

Liquid-Chromatographic Methods for Carboxylic Acids in Biological Samples

et al. 2020

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“…High-performance liquid chromatography (HPLC) is reproducible and is an invaluable tool for quantitative analysis. It has become one of the most important analytical tools in various fields such as life sciences, the food industry, and clinical research [1][2][3][4][5]. Particle-packed columns with micrometer-sized spherical particles are widely used in HPLC, and it is well known that there is a limit in their separation efficiency since it is impossible to pack the particles uniformly [6,7].…”

Section: Introductionmentioning

confidence: 99%

Development of an Automated Sample Injection System for Pillar Array Columns

et al. 2020

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A pillar array column with a perfectly ordered internal structure has a higher separation efficiency than a particle-packed column used in conventional high-performance liquid chromatography. However, applying the pillar array column technology to quantitative analysis is challenging as the injection volume of the sample varies for each injection. This occurs because the sample volumes are in the order of nanoliters and the sample is injected manually. In this study, an automated sample injection system was developed to solve this problem. The system was composed of two pumps (one for the sample and other for the mobile phase), a six-way valve that can be controlled by a PC, and an autosampler. The peak height deviation, which is more than 20% in the conventional manual injection system, was improved to 1.2% and 0.4% for the two coumarin dye samples. This result indicated that the automated sample injection method developed in this study could be applied to pillar array columns to allow for quantitative analysis.

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