Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces

Zeng, Haitong; Chittur, Krishnan K.; Lacefield, William R.

doi:10.1016/s0142-9612(98)00184-7

Cited by 192 publications

(139 citation statements)

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“…Finally, with use of the Gaussian/Lorentzian profile, each spectrum was deconvoluted into seven peaks in the 1700-1600 cm 1 region (Figure 4). According to other protein-related research, the bands can be roughly assigned as follows [42][43][44][45][46][47] antiparallel  sheet increased from 4.2% to 11.1%. The reduction in the  -helix content in favor of the  -sheet, random coil,  -antiparallel and intermolecular  -strand structure is indicative of a partial unfolding of protein in the presence of the AuNPs.…”

Section: 1mentioning

confidence: 96%

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Shi

Xie

et al. 2012

Chin. Sci. Bull.

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The interactions between bovine serum albumin (BSA) and gold nanoparticles (AuNPs), and the conformational changes of BSA induced by this interaction, were investigated by UV-visible absorption spectroscopy, fluorescence spectroscopy, and Fourier transform infrared in combination with attenuated total reflection spectroscopy (ATR-FTIR). The critical adsorption density for preventing AuNP aggregation in 0.1 mol/L phosphate buffered saline (pH 7.2) was 23 BSA molecules per gold particle or 3.8×1012 BSA molecules/cm 2 . BSA bound to the AuNPs with high affinity (binding constant K s =7.59×10 8 L/mol), and the intrinsic fluorescence of BSA was quenched by the AuNPs in accordance with the static quenching mechanism. Both fluorescence spectroscopy and ATR-FTIR showed that AuNPs induced conformational changes in BSA, which resulted in it becoming less compact and increased the polarity of the microenvironment around the tryptophan residue Trp-212.

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Section: 1mentioning

confidence: 96%

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Shi

Xie

et al. 2012

Chin. Sci. Bull.

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“…The uptake of Ca-protein complexes, which results from tight ligand binding, in the bone are higher compared to that of ionized Ca alone (Wortsman and Traycoff 1980). Other researches have also suggested that albumin bound lipids increase osteoblast growth which consequently results in bone formation (39), and albumin is used to adsorb Ca and phosphate in implant material for human body (Tsai et al 2007;Zeng et al 1999). Thus, we may expect that serum protein helps Ca and phosphate to be incorporated into the bone matrix, resulting in high bone accretion.…”

Section: Resultsmentioning

confidence: 99%

A Theoretical Modeling for Suggesting Unique Mechanism of Adolescent Calcium Metabolism

Lee

Cho

Okos

2013

Journal of Biosystems Engineering

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Purpose: Modeling has been used for elucidating the mechanism of complex biosystems. In spite of importance and uniqueness of adolescent calcium (Ca) metabolism characterized by a threshold Ca intake, its regulatory mechanism has not been covered and even not proposed. Hence, this study aims at model-based proposing potential mechanisms regulating adolescent Ca metabolism. Methods: Two different hypothetic mechanisms were proposed. The main mechanism is conceived based on Ca-protein binding which induces renal Ca filtration, while additional mechanism assumed that active renal Ca re-absorption regulated Ca metabolism in adolescents. Mathematical models were developed to represent the proposed mechanism and simulated them whether they could produce adolescent Ca profiles in serum and urine. Results: Simulation showed that both mechanisms resulted in the unique behavior of Ca metabolism in adolescents. Based on the simulation insulin-like growth factor-1 (IGF-1) is suggested as a potential regulator because it is related to both growth, a remarkable characteristic of adolescence, and Ca metabolism including absorption and bone accretion. Then, descriptive modeling is employed to conceptualize the hypothesized mechanisms governing adolescent Ca metabolism. Conclusions: This study demonstrated that modeling is a powerful tool for elucidating an unknown mechanism by simulating potential regulatory mechanisms in adolescent Ca metabolism. It is expected that various analytic applications would be plausible in the study of biosystems, particularly with combination of experimental and modeling approaches.

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“…The results showed that the MC-3T3 E1 cells are more compatible with modified surface than the pure Ti and GnG coating offered efficient communication between the implant surface and the osteoblast cells by regulating the cell signalling 45 . Zeng et al 46 modified the Ti surface with calcium phosphate (CaP) coating and protein adsorption was evaluated by FTIR. The result showed that CaP modified Ti surface adsorbed enormous quantity of protein compared with the untreated surface.…”

Section: Surface Coating For Osseointegrationmentioning

confidence: 99%

Surface Modification of Titanium and its Alloys for the Enhancement of Osseointegration in Orthopaedics

John

Jaganathan²,

Supriyanto³

et al. 2016

Current Science

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Titanium (Ti) and Ti-based alloys are the best promising orthopaedic metal transplants. The Young's modulus of Ti and bone are nearer and so Ti implants are known as osseointegrated implants. However, the need for enhancing the osseointegration, corrosion resistance and biocompatibility cannot be ruled out in promoting the Ti as a golden standard. This review describes various surface modifications like acid etching, sand blasting, surface coating, alkali-heat treatment, plasma treatment and ion implantation of Ti-based implants which are the best solutions to promote biocompatibility, osseointegration and ultimately the longevity of implants. In addition, it gives an outline to accomplish the risky task in orthopaedics like recovering skeletal function by replacing the damaged bone for human being survival and it will assist the energetic collaboration of specialists in materials science, chemistry and biology for the quality enhancement.

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Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces

Cited by 192 publications

References 0 publications

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

A Theoretical Modeling for Suggesting Unique Mechanism of Adolescent Calcium Metabolism

Surface Modification of Titanium and its Alloys for the Enhancement of Osseointegration in Orthopaedics

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