Analysis of bovine B cell reactive monoclonal antibodies

Mukwedeya, D; Takamatsu, H.; Denyer, Mick; Parkhouse, R. M. E.

doi:10.1016/0165-2427(96)05579-1

Cited by 10 publications

(5 citation statements)

References 6 publications

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“…The anti NKp46 AKS1 mAb has been raised against the bovine molecule, and its reactivity with the NK bovine subset has been well characterized (Storset et al, 2004). The DU2-104 mAb IMMUNITY IN BOVINE CLONES initially raised against sheep B cells was demonstrated to label all surface immunoglobulin positive cells in cow and is considered as a pan B-cell marker in cow (Mukwedeya et al 1996).…”

Section: Immunophenotyping Of Pbmc Subsetsmentioning

confidence: 99%

The Immune Status of Bovine Somatic Clones

Chavatte‐Palmer

Heyman

Richard³

et al. 2009

Cloning and Stem Cells

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Agronomical applications of cloned livestock produced by somatic cell nuclear transfer (SCNT) have been authorized in the United States and the European Food Safety Authority published that there was no evidence of risks associated with the use of cloned animal in the breeding industry. Both assessments, however, underlined that complementary data are needed to update their conclusions. SCNT is associated with a high incidence of perinatal losses. After birth, cloned cattle appear to possibly present subtle immune defects, requiring extensive studies to be properly evidenced. Twenty-five cloned Holstein heifers from five distinct genotypes and their contemporary age- and sex-matched controls were compared. An extensive survey of leukocyte subsets was performed and the humoral and T-cell immune responses to exogenous antigens were studied. Cloned cattle presented a normal representation of leukocyte subsets. Functional immunity was not modified in cloned heifers, as they were able to raise an antibody response and to develop B and T cell-specific responses against the model antigen OVA (ovalbumin) and against a rotavirus vaccine as in controls. Thus, this extensive analysis supports previous data suggesting that cloned cattle have a normal immunity.

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Section: Immunophenotyping Of Pbmc Subsetsmentioning

confidence: 99%

The Immune Status of Bovine Somatic Clones

Chavatte‐Palmer

Heyman

Richard³

et al. 2009

Cloning and Stem Cells

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“…Except for the former, all other MAbs were from the International Livestock Research Institute (ILRI, Nairobi, Kenya). MAb DU 2 -104, kindly provided by W. Hein, was used to detect the B-cells [17]. Flow cytometry analysis, measurement of blastogenesis, of the various cell subsets and of their state of activation (CD25 expression) have already been described [5].…”

Section: Lymphoproliferation Assays and Cell Phenotypingmentioning

confidence: 99%

Characterisation of the lymph node immune response followingMycoplasma mycoidessubsp.MycoidesSC infection in cattle

et al. 2006

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“…MoAb were obtained from the International Livestock Research Institute (ILRI, Nairobi, Kenya) except the CC15 MoAb from SEROTEC (Cergy St Christophe, France). MoAb DU 2 ‐104, kindly provided by W. Hein, was also used to detect B cells [12].…”

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confidence: 99%

Viable Mycoplasma mycoides ssp. mycoides Small Colony‐Mediated Depression of the Bovine Cell Responsiveness to the Mitogen Concanavalin A

Dedieu

Balcer-Rodrigues

2006

Scand J Immunol

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Mycoplasma mycoides ssp. mycoides biotype Small Colony (MmmSC) is the causative agent of contagious bovine pleuropneumonia (CBPP), which is still a major tropical cattle disease. Development of an efficient vaccine requires an understanding of the immunopathology of CBPP as MmmSC presents a strong ability to escape the host immune response. The objective of this study was to determine whether the presence of MmmSC can modulate the immune response induced by the mitogen Concanavalin A (ConA) on bovine immune cells [peripheral blood mononuclear cells (PBMC) and lymph node (LN) cells]. Comparative analysis of the immunomodulating properties of viable versus heat‐killed MmmSC on ConA‐stimulated immune cells revealed that while heat‐killed MmmSC had no effect, viable MmmSC strongly depressed, in a concentration‐dependent manner, the ConA mitogenic activity (blastogenesis and interferon‐γ production). Both B‐cell and T‐cell activation were affected with the highest impact on the CD4 T cells. The phenotypic analysis showed that the ConA‐induced proliferation of CD25+ cells was strongly reduced when co‐exposed to viable MmmSC, confirming that events associated with ConA‐induced cell activation were suppressed by the pathogen. This study thus demonstrated that viable MmmSC is able to inhibit the polyclonal mitogenic activity of the ConA on bovine PBMC and LN cells. This finding strongly suggests that the persistence of viable MmmSC may also thus inhibit the bovine immune response directed towards inactivated MmmSC, whether dead or in the form of antigens, also present during infection. This study confirmed that MmmSC has evolved an efficient mechanism to prevent its elimination from the host.

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Analysis of bovine B cell reactive monoclonal antibodies

Cited by 10 publications

References 6 publications

The Immune Status of Bovine Somatic Clones

The Immune Status of Bovine Somatic Clones

Characterisation of the lymph node immune response followingMycoplasma mycoidessubsp.MycoidesSC infection in cattle

Viable Mycoplasma mycoides ssp. mycoides Small Colony‐Mediated Depression of the Bovine Cell Responsiveness to the Mitogen Concanavalin A

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