Analysis of Blood Spot 17&amp;alpha;-Hydroxyprogesterone Concentration in Neonates

Shimozawa, Kazuhiko; Matsumoto, Masaru; Okada, Kodo; Murata, Mitsunori; Tsuchiya, Yoshifumi; Kitagawa, Teruo

doi:10.1159/000181071

Cited by 9 publications

(2 citation statements)

References 13 publications

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“…Four salt-losing forms, two simple virilization and two non-classical forms were diagnosed. A signifi cantly higher level of false positive results was present in preterm infants than in term infants, and in low birth weight infants than in normal birth weight infants [2], A high incidence of false-positive results in preterm infants, in sick infants and in low birth weight infants has been reported in all screening programmes for CAH [3][4][5], The methods using extraction, however, show a higher speci ficity and a lower frequency of false-positive results.…”

Section: Emilia Romagna Studymentioning

confidence: 99%

The Experience of Neonatal Screening for Congenital Adrenal Hyperplasia

Cicognani¹

1992

Horm Res

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Section: Emilia Romagna Studymentioning

confidence: 99%

The Experience of Neonatal Screening for Congenital Adrenal Hyperplasia

Cicognani¹

1992

Horm Res

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“…At the beginning of the screening program we chose 30 nmol/l as a cut-off for both preterm and term babies on the basis of the literature and the guidelines of the DELFIA kit [21,22,23]. The overall RR was 0.37%, 2.59% for preterm and 4.94% for low-birth-weight babies respectively (table 1),and, particularly, 89.6% of recalled newborns were preterm and 78.1% low-birth-weight neonates.…”

Section: Discussionmentioning

confidence: 99%

Neonatal Screening for Congenital Adrenal Hyperplasia in North-Eastern Italy: A Report Three Years into the Program

et al. 2005

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Aims: To evaluate the incidence of congenital adrenal hyperplasia (CAH) in the Northern Italian population and the efficiency of the North-Eastern Italy screening program. To adjust cut-off levels for 17-hydroxyprogesterone (17-OHP) in relation to gestational age and birth weight, comparing the benefits in terms of reduction of recall rates with the two approaches and ultimately choosing the better of the two. Subjects and Methods: Since September 2001, blood samples from neonates born in North-Eastern Italy have been screened with a fluoroimmunoassay method for 17-OHP determination (DELFIA). A preliminary cut-off level of ≧30 nmol/l was set both for term and preterm newborns. The values of 17-OHP were analysed usingstatistical methods in relation to gestational age and birth weight in order to modify the cut-off on the basis of our data. Results: After 33 months of screening we screened 128,282 newborns and detected 6 affected babies. During the first 8 months of screening among the recalled babies, 89.6 and 78.1% were preterm and low-birth-weight newborns, respectively, with a recall rate of 2.59% for premature neonates and of 4.94% for babies with birth weights <2,500 g. We chose a new cut-off value of 50 nmol/l for preterm newborns only and, after 4 months, the recall rate was reduced to 0.83% for these infantsand to 1.83% for low-birth-weight infants. Conclusion: After 33 months of screening for CAH in North-Eastern Italy, we report an incidence of 1:21,380. In 5 out of 6 affected babies, the diagnosis was established only after a positive screening test, which prevented a severe salt-wasting crisis in these babies. The cut-off level related to gestational age led to a significant reduction in the number of false-positives among preterm babies.We therefore intend to continue with the screening program for CAH in North-Eastern Italy, keeping a gestational-age-related cut-off in the hope that our data may encourage a national screening program for CAH.

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Effect of gender, birth weight and gestational age on serum 17-hydroxyprogesterone concentration and distribution among neonates in Saudi Arabia

et al. 1995

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Blood samples from 813 newborns were spotted on to filter paper and the 17-hydroxyprogesterone concentration was measured using the Delfia (R) fluorometric immunoassay. The median, mean, and standard deviation (SD) for the total population were 20, 21 and (11) nmol/L respectively. Males had significantly higher levels than females with median, mean and (SD) of 22, 22 and (12) nmol/L. Similarly, low birth weight babies were found to have significantly higher levels than normal birth weight babies with median, mean and (SD) of 21, 24 (12) nmol/L. Preterm babies were also found to have significantly higher levels than full term babies, with median, mean and (SD) of 25, 29 (16) nmol/L. As experienced elsewhere, those factors should be taken into consideration when considering a cut-off point in any neonatal screening program for congenital adrenal hyperplasia.

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Analysis of Blood Spot 17α-Hydroxyprogesterone Concentration in Neonates

Cited by 9 publications

References 13 publications

The Experience of Neonatal Screening for Congenital Adrenal Hyperplasia

The Experience of Neonatal Screening for Congenital Adrenal Hyperplasia

Neonatal Screening for Congenital Adrenal Hyperplasia in North-Eastern Italy: A Report Three Years into the Program

Effect of gender, birth weight and gestational age on serum 17-hydroxyprogesterone concentration and distribution among neonates in Saudi Arabia

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