Analysis of blood clotting with the total thrombus analysis system in healthy dogs

Abstract

To date, coagulation tests are unable to reflect in vivo coagulation status in the same system, including platelet function, fibrin clot formation, and whole blood flow. The Total Thrombus Analysis System (T-TAS), which is a microfluidic assay that simulates conditions in vivo, measures whole blood flow at defined shear rates under conditions designed to assess platelet function (PL-chip) or coagulation and fibrin clot formation (AR-chip). The T-TAS records occlusion start time, occlusion time, and area under the curve. We evaluated this test in healthy control dogs. We also investigated the effect in vivo of acetylsalicylic acid (ASA), and the effect in vitro of an anticoagulation drug (dalteparin; low-molecular-weight heparin; LMWH). The CV of the AUC of both chips was good (CVs of 6.45% [PL] and 1.57% [AR]). The inhibition of platelet function by ASA was evident in the right-shift in the PL test pressure curve. The right-shift in the AR test pressure curves showed that the administration of LMWH inhibited both platelets and the coagulation cascade. The T-TAS may be useful in the evaluation of canine blood coagulation.

Study on Chemical Constituents of the Enriched Extracts of Erigeron multiradiatus and Anti-thrombotic Effect on Zebrafish

“…Formation of thrombi is considered as the main cause of cardiovascular and cerebrovascular diseases (Alkarithi et al ., 2021). Thrombosis is a complex and progressive process involving many factors: VEC damage, changes in blood flow status, and increased blood coagulation (Iwanaga et al ., 2021; Jamiolkowski et al ., 2016; Neubauer & Zieger, 2022). Any one of these three factors can cause thrombus, but due to the complexity of the coagulation process in the blood, all three thrombosis formations are often interacting at the same time.…”

Evaluation of coagulation and platelet activation state and function in heartworm‐infected dogs

“…Platelet aggregation responses under high shear to mimic arterial blood flow were evaluated using a platelet function analyzer (PFA-100, Siemens, Inc.) and the total thrombus analysis system (T-TAS, Zacros, Inc.). 13 The T-TAS also assessed platelet function under conditions of lower shear to model venous blood flow. Whole blood coagulation and fibrinolysis were evaluated using thromboelastography (TEG).…”

“…This study evaluated platelet function and overall coagulation state in microfilaremic HWI dogs utilizing methodologies that more closely reflect in vivo conditions, comparing these assays to turbidimetric aggregometry, which uses PRP under low shear and is inherently non‐physiologic. Platelet aggregation responses under high shear to mimic arterial blood flow were evaluated using a platelet function analyzer (PFA‐100, Siemens, Inc.) and the total thrombus analysis system (T‐TAS, Zacros, Inc.) 13 . The T‐TAS also assessed platelet function under conditions of lower shear to model venous blood flow.…”