Analysis of Biomechanical Parameters in Colonic Anastomosis

Abstract

Background: The use of measures in colonic anastomoses to prevent dehiscences is of great medical interest. Sugarcane molasses, which has adequate tolerability and compatibility in vivo, has not yet been tested for this purpose. Aim: To analyze the biomechanical parameters of colonic suture in rats undergoing colectomy, using sugarcane molasses polysaccharide as tape or gel. Methods: 45 Wistar rats (Rattus norvegicus albinus) were randomized into three groups of 15 animals: irrigation of enteric sutures with 0.9% saline solution; application of sugarcane molasses polysaccharide as tape; and sugarcane molasses polysaccharide as gel. The rats underwent colon ressection, with subsequent reanastomosis using polypropylene suture; they were treated according to their respective groups. Five rats from each group were evaluated at different times after the procedure: 30, 90 and 180 days postoperatively. The following variables were evaluated: maximum rupture force, modulus of elasticity and specific deformation of maximum force. Results: The biomechanical variables among the scheduled times and treatment groups were statistically calculated. The characteristics of maximum rupture force and modulus of elasticity of the specimens remained identical, regardless of treatment with saline, polysaccharide gel or tape, and treatment time. However, it was found that the specific deformation of maximum force of the intestinal wall was higher after 180 days in the group treated with sugarcane polysaccharide gel (p=0.09). Conclusion: Compared to control, it was detected greater elasticity of the intestinal wall in mice treated with sugarcane polysaccharide gel, without changing other biomechanical characteristics, regardless of type or time of treatment.

Different Surgical Methods in Colon Anastomosis: Experimental Study

“…Anastomotic leak continues to cause serious morbidity and mortality in patients undergoing colorectal surgery, so it is difficult to treat and may require re-laparotomy 5 , 19 . Numerous experimental and clinical studies have been conducted to highlight new treatment strategies to prevent anastomotic leak and to achieve better wound healing 1 , 10 , 19 , 23 . The purpose of our study was to demonstrate the effect of an absorbable surgical barrier film on the reliability of the anastomosis by fixing the omentum and the rarely used absorbable barrier film around the anastomosis.…”

Effects of Renal Dysfunction on Healing of Colonic Anastomosis: Experimental Study in Wistar Rats

“…In patients with CKD when they need some intestinal surgical approach, even under uremic conditions, this will be the only decision to be made by the surgeon in the search for a solution for the patient, as it occurs in emergency situations or in cases of renopancreatic transplantation. Among the gastrointestinal surgical complications, the most described are the failures in anastomotic healing represented by 3.4% to 12% of dehiscence, with the main cause being the metabolic disorders of uremia secondary to CKD 16 .…”