Analysis of Biological Fluids by Direct Combination of Solid Phase Extraction and Desorption Electrospray Ionization Mass Spectrometry

Dénes, Júlia; Katona, Mária; Hosszu, Akdam; Czuczy, Noémi; Takáts, Zoltán

doi:10.1021/ac8024812

Cited by 55 publications

(36 citation statements)

References 31 publications

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“…The basic obstacles included the indispensable sample preparation, and also the incompatibility of ionization methods with the environment or the sample itself. The development of "direct" or "ambient" ionization methods has lifted some of these constraints [1], however the sensitivity of these methods ranging from laser ablation/desorption to desorption electrospray is generally poor [2,3]. It has soon been recognized that various desorption ionization methods produce a significant amount of partially charged aerosol besides gas-phase molecular ions.…”

mentioning

confidence: 99%

Electrospray Post-Ionization Mass Spectrometry of Electrosurgical Aerosols

Guenther

Schäfer

Balog³

et al. 2011

J. Am. Soc. Mass Spectrom.

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The feasibility of electrospray (ES) ionization of aerosols generated by electrosurgical disintegration methods was investigated. Although electrosurgery itself was demonstrated to produce gaseous ions, post-ionization methods were implemented to enhance the ion yield, especially in those cases when the ion current produced by the applied electrosurgical method is not sufficient for MS analysis. Post-ionization was implemented by mounting an ES emitter onto a Venturi pump, which is used for ion transfer. The effect of various parameters including geometry, high voltage setting, flow parameters, and solvent composition was investigated in detail. Experimental setups were optimized accordingly. ES post-ionization was found to yield spectra similar to those obtained by the REIMS technique, featuring predominantly lipid-type species. Signal enhancement was 20-to 50-fold compared with electrosurgical disintegration in positive mode, while no improvement was observed in negative mode. ES post-ionization was also demonstrated to allow the detection of non-lipid type species in the electrosurgical aerosol, including drug molecules. Since the tissue specificity of the MS data was preserved in the ES post-ionization setup, feasibility of tissue identification was demonstrated using different electrosurgical methods.

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mentioning

confidence: 99%

Electrospray Post-Ionization Mass Spectrometry of Electrosurgical Aerosols

Guenther

Schäfer

Balog³

et al. 2011

J. Am. Soc. Mass Spectrom.

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“…-quantitation studies for small drug molecules in the dried spots of human plasma supernatants on a glass surface [36], -quantitation of glucose by reaction DESI [37], -quantitation of amino acids from blood spots, after a rapid solvent extraction without other sample pretreatment [38], -rapid quantitative screening of clenbuterol in urine samples using DESI after solid-phase extraction [39], as well as -quantitation by DESI-MS after sample concentration on the closing frit of a solid phase extraction cartridge and analysis of this frit surface [40].…”

Section: Quantitation Of Low-molecular Components In Biological Matermentioning

confidence: 99%

“…It should be noted that the increase in sensitivity up to six orders of magnitude was reported for DESI-MS when solid-phase extraction was used for drug monitoring in human serum [40]. As another option to increase the sensitivity, derivatization techniques were applied to DESI-MS analysis of saccharides in biological fluids [37].…”

Section: Quantitation Of Low-molecular Components In Biological Matermentioning

confidence: 99%

Ambient desorption ionization mass spectrometry (DART, DESI) and its bioanalytical applications

Chernetsova

Morlock

2011

Bioanal Rev

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In recent years, ambient desorption ionization techniques for mass spectrometry were introduced. Among them, the most established techniques are Direct Analysis in Real Time (DART) and Desorption Electrospray Ionization (DESI). Therefore, the current review focuses on the bioanalytical applications of ambient desorption ionization techniques by the example of DART and DESI mass spectrometry. The potential and also limitations of both ambient mass spectrometry (MS) techniques in such areas, as identification and quantitation of small molecules, coupling DART-MS and DESI-MS with planar chromatography, protein/peptide analysis, as well as molecular imaging applications, are discussed.

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“…Elles concernent aussi bien le dosage de 5 antibiotiques [6], le dosage d'antirétro-viraux par HPLC/DAD [7] que le dosage de neuf antiépilep-tiques sur 100 μL de plasma [8], la cyclosporine sur 100 μL de plasma [9], du budénoside avec une limite de quantification de 50 pg/mL par LC/MS [10] ; le dosage de la simvastatine et de l'atorvastatine [11], du fentanyl avec une limite de quantification de 50 pg/mL par le couplage LC/MS/MS [12]. Le dosage du vigabatrin a également pu être réalisé par électrophorèse capillaire équipée d'un détecteur fluorescent [13].…”

Section: Applicationsunclassified

Extraction en phase solide (SPE) : théorie et applications

Humbert

2010

Ann Toxicol Anal

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Résumé -L'extraction en phase solide (SPE) est devenue depuis quelques années une technique de préparation d'échantillons de plus en plus utilisée en toxicologie analytique. Son principe de base est analogue à celui de la chromatographie de partage. Depuis son apparition de nombreuses phases stationnaires ont été développées par les industriels, permettant des extractions de plus en plus ciblées, ou bien adaptées pour l'extraction d'un grand nombre de substances de natures chimiques variées : pour une recherche large de xénobiotiques en milieu hospitalier pour des patients admis aux urgences ou en médecine légale dans les recherches des causes de la mort. De par la diversité des phases stationnaires elle permet l'extraction de composés polaires difficilement extractibles auparavant par les phases organiques. L'extraction se déroule généralement en quatre étapes : le conditionnement de la phase stationnaire, le chargement de l'échantillon, le(s) lavage(s) de la cartouche et enfin l'élution. La maîtrise de ce pré-analytique permet des facteurs de pré-concentration de(s) analyte(s) à rechercher ou à doser qui peut abaisser la limite de détection d'une méthode. Les larges gammes commercialisées par les industriels sont adaptées à des extractions ponctuelles de volumes très variés (de quelques dizaines de microlitres à plusieurs millilitres voire litres) mais aussi à l'extraction de grandes séries grâce à des plaques de 96 puits. C'est aussi une technique qui a l'avantage d'être automatisable. De très nombreuses mé-thodes analytiques ont été développées sur les matrices biologiques habituelles : le sérum, le plasma ou les urines mais également dans des matrices plus complexes comme les cheveux ou le méconium. Mots clés : Extraction, phase stationnaire, matrices biologiquesAbstract -Solid phase extraction (SPE) has become, in recent years, a technique for sample preparation increasingly used in analytical toxicology. Its basic principle is similar to that of partition chromatography. Since its appearance, many stationary phases have been developed by industry for more targeted extractions or adapted for the extraction of a large number of chemical substances of various kinds for a wide search for xenobiotics in hospital for patients admitted to the emergency ward, or forensic research into the causes of death. Because of the variety of stationary phases it allows the extraction of polar compounds from previously difficult to extract organic phases. The extraction is generally conducted in four steps: conditioning of the stationary phase, loading of the sample, washing(s) of the cartridge and finally, elution. Control of this pre-analytical stage allows factors of pre-concentration of the analyte(s) to search for or to be determined which can lower the limit of detection of a method. The wide ranges marketed by the industry are suitable for specific extraction of very varied volumes (a few tens of microliters up to several mL or liters) but also for extraction of large sets with plates of 96 wells. It is also a techniq...

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Analysis of Biological Fluids by Direct Combination of Solid Phase Extraction and Desorption Electrospray Ionization Mass Spectrometry

Cited by 55 publications

References 31 publications

Electrospray Post-Ionization Mass Spectrometry of Electrosurgical Aerosols

Electrospray Post-Ionization Mass Spectrometry of Electrosurgical Aerosols

Ambient desorption ionization mass spectrometry (DART, DESI) and its bioanalytical applications

Extraction en phase solide (SPE) : théorie et applications

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