Analysis of biogenic amines using corona discharge ion mobility spectrometry

The reduced mobility of the biogenic amines: trimethylamine, putrescine, cadaverine, spermidine and spermine

“…It should be noted that spermidine and spermine are not separated under these experimental conditioned and have very similar reduced [12] mobility values although they differ significantly in their molecular weight (145 and 202 Dalton, respectively). Only one peak was observed in the mobility spectrum for each of these compounds, while Hashemian et al [10] observed two peaks for each for putrescine and cadaverine. The reduced mobility values of the polyamines reported by Hashemian et al [10], with n-nonylamine as the reagent or with water based chemistry (without nonylamine as a reagent) are very high, and seem to be out of line for such heavy ions.…”

“…Also shown in Table 1are the reduced mobility values reported by Hashemian et al [10] that were measured by a linear IMS in air at 180°C, where the mobility scale was calibrated with n-nonylamine. In addition, the reduced mobility values measured in air at 200°C relative to lutidine are shown ( [11,12] and summarized by [7]).…”

“…Ion mobility spectrometry (IMS) has been used to diagnose vaginal infections or food freshness on the basis of measurement of biogenic amines, and also to demonstrate the suitability of IMS as an analytical technique for these compounds. However, the reduced mobility values reported in the literature are not consistent and differences of over 50% were found between different groups [7,10,12,13,22]. Most reported mobility measurements were carried out at a drift tube temperature of close to 200°C, where clustering and humidity effects should be minimal, yet, as shown in Table 1, large variations were found.…”

Point-of-care (POC) diagnosis of bacterial vaginosis (BV) using VGTest™ ion mobility spectrometry (IMS) in a routine ambulatory care gynecology clinic

“…Fast identification of human pathogenic bacteria was recently reported by determination of characteristic volatile organic compounds according to their normalized drift velocities in electric field MCC-IMS using radioactive or corona discharge ionization [17]. These methods and the approach of proteome-based identification by matrix-assisted laser desorption-time-of-flight mass spectrometry [17] hold interest for POC testing of bacterial cultures. So far the instruments in use are for Table 2 experimental research of low concentrations of complex bio-inorganic compounds and are neither practical nor affordable for routine diagnostic applications.…”

“…Fast identification of human pathogenic bacteria was recently reported by determination of characteristic volatile organic compounds according to their normalized drift velocities in electric field multi-capillary column-ion mobility spectrometry (MCC-IMS) using radioactive or corona discharge ionization [17]. So far the instruments in use are for experimental research of low concentrations of complex bio-inorganic compounds and are neither practical nor affordable for routine diagnostic applications.…”