Analysis of bioactive eicosanoids in equine plasma by stable isotope dilution reversed‐phase liquid chromatography/multiple reaction monitoring mass spectrometry

Mangal, Dipti; Uboh, Cornelius E.; Soma, Lawrence R.

doi:10.1002/rcm.4893

Cited by 14 publications

(15 citation statements)

References 62 publications

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“…The specific duration of the anti‐inflammatory effects of IA administration of corticosteroids has not been studied in the horse. In future studies, the analysis of bioactive eicosanoids in equine plasma (Mangal et al. , 2011) may lead to a better understanding of the actions and duration of the actions of corticosteroids in the horse.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics of dexamethasone following intra‐articular, intravenous, intramuscular, and oral administration in horses and its effects on endogenous hydrocortisone

Soma

Uboh

Liu

et al. 2012

Vet Pharm & Therapeutics

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This study investigated and compared the pharmacokinetics of intra-articular (IA) administration of dexamethasone sodium phosphate (DSP) into three equine joints, femoropatellar (IAS), radiocarpal (IAC), and metacarpophalangeal (IAF), and the intramuscular (IM), oral (PO) and intravenous (IV) administrations. No significant differences in the pharmacokinetic estimates between the three joints were observed with the exception of maximum concentration (Cmax ) and time to maximum concentration (Tmax ). Median (range) Cmax for the IAC, IAF, and IAS were 16.9 (14.6-35.4), 23.4 (13.5-73.0), and 46.9 (24.0-72.1) ng/mL, respectively. The Tmax for IAC, IAF, and IAS were 1.0 (0.75-4.0), 0.62 (0.5-1.0), and 0.25 (0.08-0.25) h, respectively. Median (range) elimination half-lives for IA and IM administrations were 3.6 (3.0-4.6) h and 3.4 (2.9-3.7) h, respectively. A 3-compartment model was fitted to the plasma dexamethasone concentration-time curve following the IV administration of DSP; alpha, beta, and gamma half-lives were 0.03 (0.01-0.05), 1.8 (0.34-2.3), and 5.1 (3.3-5.6) h, respectively. Following the PO administration, the median absorption and elimination half-lives were 0.34 (0.29-1.6) and 3.4 (3.1-4.7) h, respectively. Endogenous hydrocortisone plasma concentrations declined from a baseline of 103.8 ± 29.1-3.1 ± 1.3 ng/mL at 20.0 ± 2.7 h following the administration of DSP and recovered to baseline values between 96 and 120 h for IV, IA, and IM administrations and at 72 h for the PO.

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Section: Discussionmentioning

confidence: 99%

Pharmacokinetics of dexamethasone following intra‐articular, intravenous, intramuscular, and oral administration in horses and its effects on endogenous hydrocortisone

Soma

Uboh

Liu

et al. 2012

Vet Pharm & Therapeutics

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“…Two sample preparation methodologies were used and validated for the analysis of AA, 12-HETE, 15-HETE, 20-HETE, 11(12)-DiHETE and 14(15)-DiHETE in plasma [ 15 ]. First, to extract AA and 12 HETE a protein precipitation was applied.…”

Section: Methodsmentioning

confidence: 99%

Targeted metabolomic approach in men with carotid plaque

et al. 2018

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BackgroundThe aim of the study was to analyse the presence of several metabolites related to atherosclerosis in the plasma of patients with unstable carotid plaque and in the plasma of healthy subjects.Materials and methodsWe included 20 patients who had undergone carotid endarterectomy and 20 healthy subjects as a control group. All the subjects recruited were male. We used a metabolomic approach with liquid chromatography coupled to mass spectrometry to evaluate plasma metabolite levels in the metabolic pathway involved in the progression of atherosclerotic plaque.ResultsWe observed that circulating levels of 20-HETE were significantly higher in patients with atheroma plaque than in healthy subjects (p = 0.018). No differences were found with regard to the other metabolites analysed. We also conducted a random forest analysis and found that 20-HETE was the main differentiator in the list of selected metabolites. In addition, plasma levels of 20-HETE correlated positively with body mass index (r = 0.427, p = 0.007) and diastolic blood pressure (r = 0.365, p = 0.028).ConclusionThis study confirms that of all the molecules studied only 20-HETE is related to carotid plaque. Further studies are needed to compare patients with stable carotid plaque vs. patients with unstable carotid plaque in order to confirm that 20-HETE could be a potential factor related to carotid plaque.

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“…The analytical reproducibility, evaluated by the relative standard deviation (RSD) of seven replicates, were all less than 15%. In a previous report, the recovery rates of eicosanoid by LLE were 10.61% for TXB2, 25.56% for 11-dehydroxy TXB2, 35.19% for PGF2α, 33.21% for PGD2, 49.19% for PGE2 and 12.75% for 6-keto PGF1α extracted with two-step LLE (the first extraction was chloroform/isopropanol (2:1); the second extraction was with methyl tert-butyl ether) 32 and 22.1% for PGD2, 26.5% for PGE2, 21.1% for LTB4 and 29.5% for PGF2α when extracted with 1 M acetic acid/2-isopropanol/hexane (2:20:30, v/v/v). 23 As for the recovery rate of eicosanoid using a SPE column, more than 65% by weak anion-exchange, 0 -54% by strong anion-exchange and more than 68% by octadecylsilyl based column was reported.…”

Section: Recovery Ratesmentioning

confidence: 88%

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

Ando

Satomi

2018

ANAL. SCI.

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A simple sample preparation method for eicosanoid was developed by the combination of deproteinization and nanoLC-ESI-MS/MS. Eicosanoids are a group of bioactive lipid mediators, present in trace amounts in the body. Therefore, an analytical method for eicosanoids requires superior sensitivity. The method described in this report, which takes advantage of the highly sensitive power of nanoLC-ESI-MS/MS, enabled a simplification of the sample-preparation process. Eicosanoid extraction was performed just by homogenization in methanol with subsequent phospholipid removal, and then the liquid phase was directly subjected to nanoLC-ESI-MS/MS analysis without a condensation process. The quantitation range achieved 0.01 -100 ng/mL for thromboxane B2, and 0.05 -100 ng/mL for prostaglandin E2, prostaglandin D2, prostaglandin F2, leukotriene B4, 6-keto prostaglandin F1α and 11-dehydro thromboxane B2. Rat brain sample analyses demonstrated the feasibility of the quantification of those seven eicosanoids from biological samples.

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Analysis of bioactive eicosanoids in equine plasma by stable isotope dilution reversed‐phase liquid chromatography/multiple reaction monitoring mass spectrometry

Cited by 14 publications

References 62 publications

Pharmacokinetics of dexamethasone following intra‐articular, intravenous, intramuscular, and oral administration in horses and its effects on endogenous hydrocortisone

Pharmacokinetics of dexamethasone following intra‐articular, intravenous, intramuscular, and oral administration in horses and its effects on endogenous hydrocortisone

Targeted metabolomic approach in men with carotid plaque

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

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