Analysis of behavioral changes in zebrafish (Danio rerio) larvae caused by aminoglycoside-induced damage to the lateral line and muscles

Han, Eun‐Jung; Oh, Kwang-Jun; Park, Saemi; Rah, Yoon Chan; Park, Hae Chul; Koun, Soonil; Choi, June

doi:10.1016/j.neuro.2020.03.005

Cited by 25 publications

(22 citation statements)

References 52 publications

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“…In the 300 µM and 400 µM neomycin treatment groups, surviving larvae often exhibit pericardial edema and developmental delays. Our findings are consistent with other studies that have demonstrated off-target toxicity of compounds commonly used in lateral line research [36][37][38][39][40]. Therefore, we conclude there is a toxicity threshold for larval zebrafish at 3-4 dpf that is surpassed after four treatments when using neomycin concentrations 100 µM and above.…”

Section: Toxicity Associated With Repeated Neomycin Treatmentssupporting

confidence: 92%

Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments

Venuto¹,

Erickson²

2021

Preprint

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Acute chemical ablation of lateral line hair cells is an important tool to understand lateral line-mediated behaviors in free-swimming fish larvae and adults. However, lateral line-mediated behaviors have not been described in fish larvae prior to swim bladder inflation, possibly because single doses of ototoxin do not effectively silence lateral line function at early developmental stages. To determine if ototoxins can effectively silence the lateral line during early development, we repeatedly expose zebrafish larvae to the ototoxin neomycin during a 36-hour period from 3-4 days post-fertilization (dpf). We use simultaneous transgenic and vital dye labeling of hair cells to compare 6- hour and 12-hour repeated treatment timelines and neomycin concentrations between 0–400 µM in terms of larval survival, hair cell death, regeneration, and functional recovery. Following exposure to neomycin, we find that the emergence of newly functional hair cells outpaces cellular regeneration, likely due to the maturation of ototoxin-resistant hair cells that survive treatment. Furthermore, hair cells of 4 dpf larvae exhibit faster recovery compared to 3 dpf larvae. Our data suggest that the rapid functional maturation of ototoxin-resistant hair cells limits the effectiveness of chemical-based methods to disrupt lateral line function. Furthermore, we show that repeated neomycin treatments can continually ablate lateral line hair cells between 3–4 dpf in larval zebrafish.

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Section: Toxicity Associated With Repeated Neomycin Treatmentssupporting

confidence: 92%

Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments

Venuto¹,

Erickson²

2021

Preprint

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“…Moreover, gentamicin treatment induced the loss of neuromast hair cells in 5-day-old zebrafish, demonstrating its otoxicity [ 75 ]. When more than 70% of the hair cells were lost, Han et al [ 33 ] also observed a change in their locomotor behavior, manifested by latency of the startle response or by delayed reaction times. The oculotoxic effect of gentamicin was demonstrated in zebrafish larvae treated with 10 μM, from 3 to 5 dpf, by reduced optokinetic and visual motor responses [ 76 ].…”

Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

“…An aminoglycoside, which can induce ototoxicity and also muscle damage in zebrafish, is neomycin. Zebrafish larvae of 5 dpf exposed for one hour at 125 μM neomycin showed a significant reduction in the number of hair cells and also a change in their swimming behavior related to a decrease in the total traveled distance or in the velocity and activity ratios [ 33 ]. Streptomycin, the first member of the aminoglycoside family, is known to have the lowest toxicological effect among this group of drugs [ 102 ].…”

Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

“…Most of the research papers on this topic have reported that the zebrafish health or/and its capacity to respond to environmental changes are affected following both acute and chronic exposure to antibiotics. The toxicological effects of single and mixed antibiotics in zebrafish were studied by proteomic [ 30 ] and transcriptome sequencing [ 31 , 32 ], examination of behavioral activities [ 28 , 33 , 34 ] and biomarkers [ 35 , 36 ], histopathological analysis of various tissues [ 30 , 37 ], fish lethality assays (LC 50 ) [ 22 , 38 , 39 ] and following the fish morphological changes [ 40 ]. Antibiotics were found to interfere with the bacterial communities of zebrafish gut and with fish development, causing malformations, such as hatching delay, shorter body length, yolk sac edema, curved body axis and pericardial edema [ 23 , 25 , 41 , 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

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Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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The overuse of antibiotics combined with the limitation of wastewater facilities has resulted in drug residue accumulation in the natural environment. Thus, in recent years, the presence of antibiotic residues in the environment has raised concerns over the potential harmful effects on ecosystems and human health. The in vivo studies represent an essential step to study the potential impact induced by pharmaceutical exposure. Due to the limitations of traditional vertebrate model systems, zebrafish (Danio rerio) has recently emerged as a promising animal model to study the toxic effects of drugs and their therapeutic efficacy. The present review summarizes the recent advances made on the toxicity of seven representative classes of antibiotics, namely aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, tetracyclines and polyether antibiotics, in zebrafish, as well as the combined effects of antibiotic mixtures, to date. Despite a significant amount of the literature describing the impact of single antibiotic exposure, little information exists on the effects of antibiotic mixtures using zebrafish as an animal model. Most of the research papers on this topic have focused on antibiotic toxicity in zebrafish across different developmental stages rather than on their efficacy assessment.

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“…The length of the fast-twitch fibers was assessed using ImageJ. Phalloidin was previously used to study muscle defects in (Han et al, 2020;Jia et al, 2020;Snow et al, 2008).…”

Section: Phalloidin Staining Of Actin Fibersmentioning

confidence: 99%

Differential impact of dose-range glyphosate on locomotor behavior, neuronal activity, glio-cerebrovascular structures, and transcript regulations in zebrafish larvae

et al. 2021

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Highlights1. In zebrafish larvae, behavioral and brain electrophysiological defects elicit at high glyphosate concentrations.2. Neurological outcomes are not associated with structural neuro-vascular or muscular malformations.3. Morphological signs of microglia activation are reported after exposure to low and high glyphosate concentrations.4. Transcriptomic analysis reveals the deregulation of candidate pathways, possibly extending to neuronal vulnerability.

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Analysis of behavioral changes in zebrafish (Danio rerio) larvae caused by aminoglycoside-induced damage to the lateral line and muscles

Cited by 25 publications

References 52 publications

Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments

Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Differential impact of dose-range glyphosate on locomotor behavior, neuronal activity, glio-cerebrovascular structures, and transcript regulations in zebrafish larvae

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