Analysis of bacterial transcriptome and epitranscriptome using nanopore direct RNA sequencing
Lu Tan,
Zhihao Guo,
Yanwen Shao
et al.
Abstract:Bacterial gene expression is a complex process involving extensive regulatory mechanisms. Along with growing interests in this field, Nanopore Direct RNA Sequencing (DRS) provides a promising platform for rapid and comprehensive characterization of bacterial RNA biology. However, the DRS of bacterial RNA is currently deficient in the yield of mRNA-mapping reads and has yet to be exploited for transcriptome-wide RNA modification mapping. Here, we showed that pre-processing of bacterial total RNA (size selection… Show more
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