Analysis of Bacterial Membrane Proteins Produced During Mammalian Infection Using Hydrophobic Antigen Tissue Triton Extraction (HATTREX)

Abstract

Hydrophobic antigen tissue Triton extraction (HATTREX) provides a method to extract and identify hydrophobic bacterial membrane proteins from host‐infected tissues. The non‐ionic detergent Triton X‐114 is used to solubilize host‐infected tissues at 4°C. Subsequent phase partitioning of Triton X‐114 extracted material at 37°C results in a “detergent poor” aqueous phase and a “detergent rich” detergent phase. While soluble proteins partition to the aqueous phase, hydrophobic proteins, such as proteins of the outer and inner membranes, can be found in the detergent phase. Characterization of the detergent phase sample provides insights into the proteome of bacterial membranes during infection. Curr. Protoc. Microbiol. 9:12.1.1‐12.1.5. © 2008 by John Wiley & Sons, Inc.

A liquid chromatography‐tandem mass spectrometry‐based targeted proteomics approach for the assessment of transferrin receptor levels in breast cancer

“…Our lab has successfully applied Triton X‐114 to the extraction of P‐glycoprotein from cells . Notably, while Triton X‐114 has been employed often for the extraction of membrane proteins from cultured cells, its application to tissue samples has been reported only rarely . In this study, this detergent was used to extract membrane proteins from breast tissue samples.…”

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