Analysis of bacterial community profiles of endodontically infected primary teeth using pyrosequencing

Yun, Kwan Hee; Lee, Hyo-Seol; Nam, Ok Hyung; Moon, Chan Yang; Lee, Jae Hyung; Choi, Sung Chul

doi:10.1111/ipd.12226

Cited by 14 publications

(30 citation statements)

References 47 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was the pathogen that could cause periapical infection [43][44][45], often found in deep dentin caries [30,46] and infected root canals [42]. Similarly, Fusobacterium was often detected in deep dentin caries [30,46] and infected root canals [44,45,47], It was a strict obligate anaerobe, with negative gram staining, normally living in oral cavity, upper digestive tract, intestinal tract, urogenital tract of human or animal and soil. It was most commonly seen in oral dental plaque.…”

Section: Discussionmentioning

confidence: 99%

Effect of silver fluoride on micro-ecology of plaque from extensive caries of primary teeth - in vitro study

Liu¹,

Liu

ZHANG

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The action mechanism of silver diammine fluoride (SDF) on plaque micro-ecology was seldom studied. This study investigated micro-ecological changes in dental plaque on extensive carious cavity of deciduous teeth after topical SDF treatment. Methods Deciduous teeth with extensive caries freshly removed from school children were collected in clinic. After initial plaque collection, each cavity was topically treated with 38% SDF in vitro. Repeated plaque collections were done at 24 hours and 1 week post-intervention. Post-intervention micro-ecological changes including microbial diversity, microbial metabolism function as well as inter-microbial connections were analyzed and compared after Pyrosequencing of the DNA from the plaque sample using Illumina MiSeq platform. Results After SDF application, microbial diversity decreased (p>0.05). Microbial community composition post-intervention was obviously different from that of supragingival and pre-intervention plaque as well as saliva. At 1 week post-intervention, the relative content of Pseudomonas , Fusobacterium and Pseudoramibacter was obviously higher than before, while most of the other bacteria was obviously reduced, although not statistically significant (P>0.05). The inter-microbial connections became more complex, with positive connections overcame negative ones. Carbohydrate transportation and metabolic functions in the plaque were significantly reduced at 24 hours and 1 week post-intervention. Conclusions SDF has extensive antimicrobial effect on dental plaque, which may reduce carbohydrate metabolism in dental plaque and help promote new balance of the plaque flora.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of silver fluoride on micro-ecology of plaque from extensive caries of primary teeth - in vitro study

Liu¹,

Liu

ZHANG

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Similarly, Fusobacterium is often detected in deep dentin caries [30,45] and infected root canals [43,44,46]. It is a strictly anaerobic Gram-negative bacillus living in the oral cavity and the upper digestive, intestine and urogenital tracts of humans or animals, as well as in the soil.…”

Section: Discussionmentioning

confidence: 99%

Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study

Liu¹,

Liu

ZHANG

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The mechanism of action of silver diammine fluoride (SDF) on plaque micro-ecology is seldom studied. This study investigated micro-ecological changes in dental plaque on extensive caries of deciduous teeth after topical SDF treatment. Methods: Deciduous teeth with extensive caries freshly removed from school children were collected in clinic. Unstimulated saliva collection and initial plaque sampling were done before tooth extraction, then each caries was topically treated with 38% SDF in vitro. After intervention, each tooth was stored respectively in artificial saliva at 37℃. Repeated plaque collections were done at 24 hours and 1 week post-intervention. Post-intervention micro-ecological changes including microbial diversity, microbial metabolism function as well as species correlations were analyzed and compared after pyrosequencing of the DNA from the plaque sample using Illumina MiSeq platform. Results: After SDF application, microbial diversity decreased (P>0.05), although not statistically significant. Microbial community composition post-intervention was noticeably different from that of supragingival and pre-intervention plaque as well as saliva. At 1 week post-intervention, the relative content of Pseudomonas, Fusobacterium and Pseudoramibacter was noticeably higher than before, while most of the other bacteria was noticeably reduced, although not statistically significant (P>0.05). The inter-microbial associations became more complex, much more positive associations among survived bacteria were observed than negative ones. COG function classification diagram showed carbohydrate transportation and metabolic functions in the plaque were significantly reduced at 24 hours and 1 week post-intervention. Conclusions: SDF has extensive antimicrobial effect on dental plaque, which may reduce carbohydrate metabolism in dental plaque and help promote new balance of the plaque flora.

show abstract

“…Only teeth from adult patients with carious lesions, necrotic pulps, and radiographic evidence of apical periradicular disease were involved in this study. We excluded patients who had systemic disease, cancer, diabetes, immunodeficiency disorder, and a history of using antibiotics or fluoride in the previous 3 months [ 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…Afterwards, the samples were transferred to the laboratory for DNA extraction in frozen ice packs. A negative control (sample code 19) using sterile file and paper points which were not applied to the root canal was used in parallel to test for the existence of contaminants in the file and paper points [ 19 , 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…The eluted DNA product obtained after purification was quantified using Qubit Kit Assays (Invitrogen, Life Technologies). Bioanalyzer 2100 with the DNA 1000 Chip kit (Agilent, Palo Alto, CA, USA) was used to assess the quality of the final products for each sample individually [ 19 , 24 ]. There was no amplification product observed in the negative extraction controls.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Illumina MiSeq Sequencing for Preliminary Analysis of Microbiome Causing Primary Endodontic Infections in Egypt

Tawfik

Azab

Ahmed

et al. 2018

International Journal of Microbiology

View full text Add to dashboard Cite

The use of high throughput next generation technologies has allowed more comprehensive analysis than traditional Sanger sequencing. The specific aim of this study was to investigate the microbial diversity of primary endodontic infections using Illumina MiSeq sequencing platform in Egyptian patients. Samples were collected from 19 patients in Suez Canal University Hospital (Endodontic Department) using sterile # 15K file and paper points. DNA was extracted using Mo Bio power soil DNA isolation extraction kit followed by PCR amplification and agarose gel electrophoresis. The microbiome was characterized on the basis of the V3 and V4 hypervariable region of the 16S rRNA gene by using paired-end sequencing on Illumina MiSeq device. MOTHUR software was used in sequence filtration and analysis of sequenced data. A total of 1858 operational taxonomic units at 97% similarity were assigned to 26 phyla, 245 families, and 705 genera. Four main phyla Firmicutes, Bacteroidetes, Proteobacteria, and Synergistetes were predominant in all samples. At genus level, Prevotella, Bacillus, Porphyromonas, Streptococcus, and Bacteroides were the most abundant. Illumina MiSeq platform sequencing can be used to investigate oral microbiome composition of endodontic infections. Elucidating the ecology of endodontic infections is a necessary step in developing effective intracanal antimicrobials.

show abstract

Analysis of bacterial community profiles of endodontically infected primary teeth using pyrosequencing

Abstract: This study revealed a relationship between the oral microbiome and endodontic infection in primary teeth. Additionally, anaerobic bacteria such as Fusobacterium species were dominant in the teeth with apical abscesses.

Cited by 14 publications

References 47 publications

Effect of silver fluoride on micro-ecology of plaque from extensive caries of primary teeth - in vitro study

Effect of silver fluoride on micro-ecology of plaque from extensive caries of primary teeth - in vitro study

Effect of silver diammine fluoride on micro-ecology of plaque from extensive caries of deciduous teeth - in vitro study

Illumina MiSeq Sequencing for Preliminary Analysis of Microbiome Causing Primary Endodontic Infections in Egypt

Contact Info

Product

Resources

About