Analysis of N‐acyl‐<scp>L</scp>‐homoserine lactones produced by Burkholderia cepacia with partial filling micellar electrokinetic chromatography – electrospray ionization‐ion trap mass spectrometry

Frommberger, Moritz; Schmitt‐Kopplin, Philippe; Menzinger, F.; Albrecht, Valérie; Schmid, Michael; Eberl, Leo; Hartmann, Anton; Kettrup, A.

doi:10.1002/elps.200305567

Cited by 46 publications

(33 citation statements)

References 44 publications

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“…The small volume of the analyte needed in CE experiments has made possible the measurement of the cellular contents (native proteins [357], specific fluorescent-labelled or-induced proteins [358], metabolites), the monitoring of single cells [359,360] or bacteria communications [361]. Different CE-techniques like CGE or CIEF were developed over the last years and are currently used intensively in a similar manner to classical gel electrophoresis [362] in the fields of human genomics and proteomics [363,364].…”

Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

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282

232

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Capillary electrophoresis -mass spectrometry: 15 years of developments and applicationsSince its introduction in 1987, capillary electrophoresis-mass spectrometry (CE-MS) has developed to a well accepted multidimensional analytical approach complementary and/or competitive to classical MS-hyphenated separation techniques. The threefold combination of rapid developments of an exceptional separation technique, of selective mass detection possibilities, and of very mild ionization modes first allowed these progresses. This article shows the CE specificities that need to be well controlled/known, compared to classical and more routinely used liquid chromatography in the light of its coupling to MS. The major trends and developments over the last 15 years and most of the reviews and applications found in ISI Web of science and publisher databases are presented in a tabulated way. The reader can thus rapidly find existing CE-MS analysis techniques in his field of research and application (forensics, environment, bioanalytics, pharmaceutics, and metabolites).

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Section: Discussionmentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

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282

232

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“…Cultures were stimulated with the gram-negative quorum-sensing molecule N-butyryl-L-homoserine lactone (C4HSL) at 200 μM, or N-3-oxo-dodecanoyl-L-homoserine lactone (C12HSL) at 100 μM, physiological acyl-homoserine lactone (AHL) concentrations observed during late stationary planktonic phase and biofilm growth (45). Both C12HSL and C4HSL are produced by the important respiratory pathogen P. aeruginosa as well as other gram-negative bacteria (46)(47)(48), which may use these molecules for interspecies communication to form mixed biofilms on the airway epithelium (49). Cultures derived from individuals homozygous for functional T2R38 (PAV/PAV) had a significantly greater Ca 2+ response to the AHLs compared with those homozygous or heterozygous for the nonfunctional receptor (AVI/AVI or PAV/AVI, respectively) ( Figure 2, C-F).…”

Section: Figurementioning

confidence: 99%

T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection

Lee

Xiong²,

Kofonow³

et al. 2012

J. Clin. Invest.

494

1,003

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Innate and adaptive defense mechanisms protect the respiratory system from attack by microbes. Here, we present evidence that the bitter taste receptor T2R38 regulates the mucosal innate defense of the human upper airway. Utilizing immunofluorescent and live cell imaging techniques in polarized primary human sinonasal cells, we demonstrate that T2R38 is expressed in human upper respiratory epithelium and is activated in response to acyl-homoserine lactone quorum-sensing molecules secreted by Pseudomonas aeruginosa and other gram-negative bacteria. Receptor activation regulates calcium-dependent NO production, resulting in stimulation of mucociliary clearance and direct antibacterial effects. Moreover, common polymorphisms of the TAS2R38 gene were linked to significant differences in the ability of upper respiratory cells to clear and kill bacteria. Lastly, TAS2R38 genotype correlated with human sinonasal gram-negative bacterial infection. These data suggest that T2R38 is an upper airway sentinel in innate defense and that genetic variation contributes to individual differences in susceptibility to respiratory infection.

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“…For this nontarget screening approach, we choose GC-MS because of its good separation power, sensitivity and better structural information obtained in the mass spectra compared to the more often used HPLC-MS or HPLC-MS/MS methods. Other approaches with CE, [17] GC-MS, [18] or HPLC-MS [19] use target-oriented analyses to identify known AHLs or are restricted to certain classes of AHLs. [20] Initially the methanol from the resin extracts of active cultures was removed by evaporation, and the solid material was resuspended with ethyl acetate to remove unsoluble matrix material in the sample so as to facilitate GC-MS analysis.…”

Section: Gc-ms Screening Of Active Culture Supernatantsmentioning

confidence: 99%

Discovery of Complex Mixtures of Novel Long‐Chain Quorum Sensing Signals in Free‐Living and Host‐Associated Marine Alphaproteobacteria

Wagner‐Döbler¹,

Thiel

Eberl³

et al. 2005

ChemBioChem

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171

212

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More than 100 bacterial isolates from various marine habitats were screened for AHL production by using gfp reporter constructs based on the lasR system of Pseudomonas aeruginosa and the luxR system of Vibrio fischeri. Of the 67 Alphaproteobacteria tested, most of which belonged into the so-called Roseobacter clade, 39 induced fluorescence in either one or both sensor strains up to 103-fold compared to controls. Acylated homoserine lactones were identified by GC-MS analysis and shown to have chain lengths of C8, C10, C13-C16, and C18. One or two double bonds were often present, while a keto or hydroxyl group occurred only rarely in the side chain. Most strains produced several different AHLs. C18-en-HSL and C18-dien-HSL were produced by Dinoroseobacter shibae, an aerobic anoxygenic phototrophic bacterium isolated from dinoflagellates, and are among the longest AHLs found to date. Z7-C14-en-HSL, which has previously been detected in Rhodobacter sphaeroides, was produced by Roseovarius tolerans and Jannaschia helgolandensis. This signal molecule was synthesised and shown to induce a similar response to the culture supernatant in the respective sensor strain. The widespread occurrence of quorum-sensing compounds in marine Alphaproteobacteria, both free-living strains and those associated to eukaryotic algae, points to a great importance of this signalling mechanism for the adaptation of the organisms to their widely different ecological niches.

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Analysis of N‐acyl‐L‐homoserine lactones produced by Burkholderia cepacia with partial filling micellar electrokinetic chromatography – electrospray ionization‐ion trap mass spectrometry

Cited by 46 publications

References 44 publications

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection

Discovery of Complex Mixtures of Novel Long‐Chain Quorum Sensing Signals in Free‐Living and Host‐Associated Marine Alphaproteobacteria

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