Analysis of axonal trafficking via a novel live imaging technique reveals distinct Hedgehog transport kinetics

Daniele, Joseph R.; Baqri, Rehan M.; Kunes, Sam

doi:10.1242/bio.024075

Cited by 9 publications

(4 citation statements)

References 45 publications

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“…c Assessment of axonal targeting with knockdown of 16p11.2 homologs. The schematic of the third-instar larval visual system was generated by Sam Kunes 68 and reprinted with permission from the publisher. Representative confocal images of larval eye discs stained with anti-chaoptin illustrate normal axonal targeting from the retina to the optic lobes of the brain in the control and defects with eye-specific knockdown of KCTD13 CG10465 and MAPK3 rl (scale bar = 10 µm).…”

Section: Resultsmentioning

confidence: 99%

Pervasive genetic interactions modulate neurodevelopmental defects of the autism-associated 16p11.2 deletion in Drosophila melanogaster

et al. 2018

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As opposed to syndromic CNVs caused by single genes, extensive phenotypic heterogeneity in variably-expressive CNVs complicates disease gene discovery and functional evaluation. Here, we propose a complex interaction model for pathogenicity of the autism-associated 16p11.2 deletion, where CNV genes interact with each other in conserved pathways to modulate expression of the phenotype. Using multiple quantitative methods in Drosophila RNAi lines, we identify a range of neurodevelopmental phenotypes for knockdown of individual 16p11.2 homologs in different tissues. We test 565 pairwise knockdowns in the developing eye, and identify 24 interactions between pairs of 16p11.2 homologs and 46 interactions between 16p11.2 homologs and neurodevelopmental genes that suppress or enhance cell proliferation phenotypes compared to one-hit knockdowns. These interactions within cell proliferation pathways are also enriched in a human brain-specific network, providing translational relevance in humans. Our study indicates a role for pervasive genetic interactions within CNVs towards cellular and developmental phenotypes.

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Section: Resultsmentioning

confidence: 99%

Pervasive genetic interactions modulate neurodevelopmental defects of the autism-associated 16p11.2 deletion in Drosophila melanogaster

et al. 2018

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“…Yazawa et al [29] proposed that Hedgehog proteins in the CNS could be transported posteriorly via axonal trafficking [48] where they are secreted, stimulating Wnt expression in differentiated cells (e.g. myocytes) that in turn signal to neighbouring cells, causing up-regulation of β-catenin and a feedback loop that results in posteriorisation.…”

Section: Discussionmentioning

confidence: 99%

Segmentation in tapeworms as a modified form of flatworm posterior regeneration involving Wnt and Hedgehog signalling

Jarero,

Baillie,

Riddiford

et al. 2023

Preprint

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Tapeworms are parasitic flatworms that lack key features conventionally used to define the head-tail axis in free-living organisms, resulting in long standing questions regarding the true orientation of their main body axis. As adults, most tapeworms also exhibit a segmented body which has been considered an adaptation unique to the group. Anteroposterior (AP) patterning in free-living flatworms is controlled by β-catenin-dependent Wnt signalling and positional control genes are expressed by their musculature in highly regionalised domains. Here we investigate the expression of Wnt and Hedgehog components during the strobilar phase of the tapeworm life cycle during which larval tissues are lost and replaced through the continuous production of new tissues. Results reveal previously unidentified centres of signalling associated with their neuromuscular system and show that segments are marked by secondary, AP axes in agreement with the polarity of the primary body axis. Neuromuscular expression and communication between Wnt and Hedgehog signalling is consistent with embryonic and regenerative growth in planarians and is a common mechanism for establishing AP-polarised boundaries in a diverse range of segmented animals. Taken together our results suggest that segmentation in tapeworms represents a modified form of posterior regeneration, a common feature among flatworms.

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“…Kymographs are largely used to investigate particle velocity [1,2,3,4,5]. Kymographs are 2D graphical representations of the spatial position of an object (particle etc.)…”

Section: Introductionmentioning

confidence: 99%

SHOT-R: A next generation algorithm for particle kinematics analysis

Corradi

Boscheri

Baudet

2021

Preprint

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Analysis of live-imaging experiments is crucial to decipher a plethora of cellular mechanisms within physiological and pathological contexts. Kymograph, i.e. graphical representations of particle spatial position over time, and single particle tracking (SPT) are the currently available tools to extract information on particle transport and velocity. However, the spatiotemporal approximation applied in particle trajectory reconstruction with those methods intrinsically prevents an accurate analysis of particle kinematics and of instantaneous behaviours. Here, we present SHOT-R, a novel numerical method based on polynomial reconstruction of 4D (3D+time) particle trajectories. SHOT-R, contrary to other tools, computes bona fide instantaneous and directional velocity, and acceleration. Thanks to its high order continuous reconstruction it allows, for the first time, kinematics analysis of co-trafficked particles. Overall, SHOT-R is a novel, versatile, and physically reliable numerical method that achieves all-encompassing particle kinematics studies at unprecedented accuracy on any live-imaging experiment where the spatiotemporal coordinates can be retrieved.

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Analysis of axonal trafficking via a novel live imaging technique reveals distinct Hedgehog transport kinetics

Cited by 9 publications

References 45 publications

Pervasive genetic interactions modulate neurodevelopmental defects of the autism-associated 16p11.2 deletion in Drosophila melanogaster

Pervasive genetic interactions modulate neurodevelopmental defects of the autism-associated 16p11.2 deletion in Drosophila melanogaster

Segmentation in tapeworms as a modified form of flatworm posterior regeneration involving Wnt and Hedgehog signalling

SHOT-R: A next generation algorithm for particle kinematics analysis

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