Analysis of Ascorbic Acid and Related Compounds in Fluids and Tissues

An electrochemical method has been developed for the determination of L-ascorbic acid (AsA) by a flow injection analysis. The method is based on a reaction of AsA with copper(II) at pH 5.0 to form a readily oxidizable species, followed by electrochemical detection at an applied potential of +20 mV vs. Ag/AgCI. Under the optimum conditions a linear calibration curve was obtained for AsA over the concentration range from 0.1 to 20 µM with a sample volume of 20 µl. The relative standard deviation of the peak height for 10 µM AsA was 1.0% (n=10). The method was successfully applied to the determination of AsA in fresh fruit juices and soft drinks. The obtained results are in good agreement with those obtained by high-performance liquid chromatography (r=0.994).

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Analysis of Ascorbic Acid and Related Compounds in Fluids and Tissues

Cited by 3 publications

References 120 publications

Biospecific methods for the determination of water-soluble vitamins

Biospecific methods for the determination of water-soluble vitamins

Chemistry of L-ascorbic acid related to foods

Determination of l-Ascorbic Acid by a Flow Injection Analysis with Copper(II)-Mediated Electrochemical Detection

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