Analysis of aqueous 2,4,6-trinitrotoluene (TNT) using a fluorescent displacement immunoassay

Abstract

We report a rapid, simple, and sensitive assay that is potentially amenable to high throughput screening for analysis of 2,4,6-trinitrotoluene (TNT) present in aqueous solutions. The assay is based on the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody that occurs upon its competitive displacement by TNT. The assay can be performed in both cuvette- and 96-well plate-based formats. TNT at a level of 0.5 micro g L(-1) (0.5 ppb) was detected in phosphate buffered saline; detection improved to 0.05 micro g L(-1) (0.05 ppb) for TNT dissolved in artificial seawater.

Detection of 2,4,6-Trinitrotoluene in Environmental Samples Using a Homogeneous Fluoroimmunoassay

“…An examination of cross-reactivities with other nitroaromatics and other explosives in this assay showed that the results are consistent with the cross-reactivity profile of the antibody used in the assay (22). This homogeneous fluoroimmunoassay has worked with five monoclonal anti-TNT antibodies examined as well as with several anti-TNT singlechain antibody Fv fragments (ERG, TC unpublished observation).…”

Sensors—An effective approach for the detection of explosives

“…Goldman et al [75] reported a rapid, simple and sensitive assay for the analysis of TNT. The basis of assay is the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody.…”

Microscopic determination of tetracycline based on aluminum-sensitized fluorescence of a self-ordered ring formed by a sessile droplet on glass slide support

“…Both common metal ions in urine, such as Na + , K + , Ca 2+ , NH 4 + , and glucide, urea can be allowed with high concentration level above 1.0 × 10 −4 mol l −1 . Whereas, substances including Mg 2+ , Fe 3+ , Cu 2+ , Ni 2+ , Cr 3+ , Mn 2+ , PO 4 3− , amino acids, lactonflavin, nicotinamide, calcium pantothenate can be allowed with concentration level above 1.0 × 10 −6 mol l −1 , about 10-100 fold higher than the allowed levels reported by other authors [33]. Biological molecules such as bovine serum albumin (BSA) and calf thymus DNA (ctDNA) can be allowed at 10 and 3.8 g ml −1 , respectively, which supplies a good permission for the assay of tetracycline in body fluid samples.…”