Analysis of antioxidative factors related to <scp>AMD</scp> risk development in the polish patients

Mrowicka, Małgorzata; Mrowicki, Jerzy; Szaflik, Jacek P.; Szaflik, Marta; Ulińska, Magdalena; Szaflik, Jerzy; Majsterek, Ireneusz

doi:10.1111/aos.13289

Cited by 16 publications

(12 citation statements)

References 32 publications

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“…First, we assessed the systemic levels of components of the antioxidant system in peripheral blood and found that the activity of the majority of tested substances were significantly increased in patients with AMD (GPx, R-GSSG, and GSH transferase levels in RBCs and SOD, CAT, GPx, and R-GSSG levels in PLT), whereas only the CAT activity in RBC was evidently reduced in patients with AMD compared with controls. Our observation of increased GPx activity is in contrast to the results reported by Mrowicka et al [ 44 ] and Plestina-Borjan et al [ 31 ], where significantly lower GPx (RBC) activity was observed in AMD patients in comparison with controls. On the other hand, in the large POLA study of a cohort of 2584 participants, the increased levels of plasma GPx concentration, which catalyses H 2 O 2 degradation by GSH, correlated with a nine-fold increase in the prevalence of late AMD [ 45 ].…”

Section: Discussioncontrasting

confidence: 99%

“…Similar to SOD, CAT function decreases in the macular and peripheral RPE with ageing [ 58 ], but in contrast to SOD, CAT immunoreactivity is reduced in RPE cells in the eyes of patients with and without AMD [ 47 , 59 ]. Our finding of reduced CAT activity in patients with AMD is consistent with previous reports [ 44 ]. As proposed by Tate et al, treatment with ROS-generating compounds induces CAT expression in RPE cells, which protects against H 2 O 2 , even in the adjacent RPE cells without upregulated CAT expression.…”

Section: Discussionsupporting

confidence: 93%

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Dietary and Lifestyle Factors Modulate the Activity of the Endogenous Antioxidant System in Patients with Age-Related Macular Degeneration: Correlations with Disease Severity

et al. 2020

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Age-related macular degeneration (AMD) is a common cause of blindness in the elderly population, but the pathogenesis of this disease remains largely unknown. Since oxidative stress is suggested to play a major role in AMD, we aimed to assess the activity levels of components of the antioxidant system in patients with AMD. We also investigated whether lifestyle and dietary factors modulate the activity of these endogenous antioxidants and clinical parameters of disease severity. We recruited 330 patients with AMD (39 with early, 100 with intermediate and 191 with late form of AMD) and 121 controls in this study. At enrolment, patients’ dietary habits and physical activity were assessed, and each study participant underwent a thorough ophthalmologic examination. The activity of several components of the antioxidant system were measured in red blood cells and platelets using both kinetic and spectrophotometric methods. Patients with AMD consumed much lower levels of fatty fish and eggs than the control group (p = 0.008 and p = 0.04, respectively). In the nAMD group, visual acuity (VA) correlated positively with green vegetable consumption (Rs = +0.24, p = 0.004) and omega-3-rich oil intake (Rs = +0.17, p = 0.03). In the AMD group, the total physical activity MET score correlated positively with VA (Rs = +0.17, p = 0.003) and correlated negatively with the severity of AMD (Rs = −0.14, p = 0.01). A multivariate analysis of patients and controls adjusted for age, sex, and smoking status (pack-years) revealed that AMD was an independent variable associated with a lower RBC catalase (β = −0.37, p < 0.001) and higher PLT catalase (β = +0.25, p < 0.001), RBC GPx (β = +0.26, p < 0.001), PLT GPx (β = +0.16, p = 0.001), RBC R-GSSG (β = +0.13, p = 0.009), PLT R-GSSG (β = +0.12, p = 0.02) and RBC GSH transferase (β = +0.23, p < 0.001) activity. The activities of components of the antioxidant system were associated with disease severity and depended on dietary habits. The observed substantial increase in the activity of many critical endogenous antioxidants in patients with AMD further indicates that the required equilibrium in the antioxidant system is disturbed throughout the course of the disease. Our findings explicitly show that a diet rich in green vegetables, fish and omega-3-rich oils, supplemented by physical exercise, is beneficial for patients with AMD, as it might delay disease progression and help retain better visual function.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 93%

Dietary and Lifestyle Factors Modulate the Activity of the Endogenous Antioxidant System in Patients with Age-Related Macular Degeneration: Correlations with Disease Severity

et al. 2020

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“…First, oxidative stress is a well-established mechanism that contributes to cellular dysfunction/cell death, including photoreceptor death. Oxidative stress has been reported in development and acceleration of many retinal diseases, including retinitis pigmentosa (11,15,52,79), AMD (80)(81)(82), glaucoma (83)(84)(85), and diabetic retinopathy (86,87). Second, TH has a profound effect on mitochondrial function/metabolic homeostasis.…”

Section: Th Signaling Suppression-induced Cone Protection Involves Thmentioning

confidence: 99%

Deficiency of type 2 iodo‐ thyronine deiodinase reduces necroptosis activity and oxidative stress responses in retinas of Leber congenital amaurosis model mice

Yang

Butler

et al. 2018

FASEB j.

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Thyroid hormone (TH) signaling has been shown to regulate cone photoreceptor viability. Suppression of TH signaling with antithyroid drug treatment or by targeting iodothyronine deiodinases and TH receptors preserves cones in mouse models of retinal degeneration, including the Leber congenital amaurosis Rpe65-deficient mice. This work investigates the cellular mechanisms underlying how suppressing TH signaling preserves cones in Rpe65-deficient mice, using mice deficient in type 2 iodothyronine deiodinase (Dio2), the enzyme that converts the prohormone thyroxine to the active hormone triiodothyronine (T3). Deficiency of Dio2 improved cone survival and function in Rpe65 and Rpe65-deficiency on a cone dominant background ( Rpe65/ Nrl) mice. Analysis of cell death pathways revealed that receptor-interacting serine/threonine-protein kinase (RIPK)/necroptosis activity was increased in Rpe65/ Nrl retinas, and Dio2 deficiency reversed the alterations. Cell-stress analysis showed that the cellular oxidative stress responses were increased in Rpe65/ Nrl retinas, and Dio2 deficiency abolished the elevations. Similarly, antithyroid drug treatment resulted in reduced RIPK/necroptosis activity and oxidative stress responses in Rpe65/ Nrl retinas. Moreover, treatment with T3 significantly induced RIPK/necroptosis activity and oxidative stress responses in the retina. This work shows that suppression of TH signaling reduces cellular RIPK/necroptosis activity and oxidative stress responses in degenerating retinas, suggesting a mechanism underlying the observed cone preservation.-Yang, F., Ma, H., Butler, M. R., Ding, X.-Q. Deficiency of type 2 iodothyronine deiodinase reduces necroptosis activity and oxidative stress responses in retinas of Leber congenital amaurosis model mice.

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“…While exogenous micronutrient supplementation protective role has been unequivocally demonstrated in the landmark AREDS and AREDS2 studies (Age-Related Eye Disease Study Research Group 2001, Age-Related Eye Disease Study 2 Research Group 2013), interestingly, not only prevention but also visual function may be improved by supplementation, with a clinical trial using antioxidant supplementation in an Italian population having shown improved visual acuity (VA) and contrast sensitivity (Piermarocchi et al 2012). While not much is known regarding naturally occurring dietary nutrients and their role as a modifiable risk factor, insufficient antioxidant capacity may have an important role in AMD (Mrowicka et al 2017). In our exploratory analysis, we have targeted those micronutrients more frequently described in the literature.…”

Section: Dietary Micronutrientsmentioning

confidence: 99%

Adherence to a Mediterranean diet, lifestyle and age‐related macular degeneration: the Coimbra Eye Study – report 3

Raimundo

Mira

Cachulo

et al. 2018

Acta Ophthalmologica

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Analysis of antioxidative factors related to AMD risk development in the polish patients

Abstract: In conclusion, our data showed that insufficient antioxidant capacity may have an important role in age-related macular degeneration. The polymorphism of GPx Pro197Leu may reduce the ability to scavenge free radicals in retina and contribute to the development of AMD.

Cited by 16 publications

References 32 publications

Dietary and Lifestyle Factors Modulate the Activity of the Endogenous Antioxidant System in Patients with Age-Related Macular Degeneration: Correlations with Disease Severity

Dietary and Lifestyle Factors Modulate the Activity of the Endogenous Antioxidant System in Patients with Age-Related Macular Degeneration: Correlations with Disease Severity

Deficiency of type 2 iodo‐ thyronine deiodinase reduces necroptosis activity and oxidative stress responses in retinas of Leber congenital amaurosis model mice

Adherence to a Mediterranean diet, lifestyle and age‐related macular degeneration: the Coimbra Eye Study – report 3

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