Analysis of Antioxidant Lipids in Five Species of Dietary Seaweeds by Liquid Chromatography/Mass Spectrometry

Gowda, Siddabasave Gowda B.; Chen, Yifan; Gowda, Divyavani; Tsuboi, Yui; Chiba, Hitoshi; Hui, Shu‐Ping

doi:10.3390/antiox11081538

Cited by 10 publications

(8 citation statements)

References 56 publications

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“…Data processing used MS-DIAL software version 4.9, which plays a pivotal role in tasks such as data alignment, peak extraction, identification, and peak area integration. 41 The minimum peak height of 1000 amplitude, maximum charged number 2, smoothing level and minimum peak width 3 and 5 scans, mass slice width 0.1 Da, σ window value 0.5, and the identification score cutoff was set to 80%. Further, signal intensity 5 folds greater than the blank was selected with retention time (alignment) and MS 1 tolerance 0.1 min and 0.015 Da, respectively.…”

Section: Methodsmentioning

confidence: 99%

Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS

Jayaprakash,

B. Gowda,

K. Shukla

et al. 2024

ACS Omega

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Consumption of alcohol has widespread effects on the human body. The organs that are most significantly impacted are the liver and digestive system. When alcohol is consumed, it is absorbed in the intestines and processed by the liver. However, excessive alcohol use may affect gut epithelial integrity, microbiome composition, and lipid metabolism. Despite past studies investigating the effect of ethanol on hepatic lipid metabolism, the focus on colonic lipid metabolism has not been well explored. In this study, we investigated the sex-specific effect of ethanol on the colonic content lipidome in a mouse model using nontargeted liquid chromatography−mass spectrometry. Comprehensive lipidome analysis of colonic flush samples was performed using ethanolfed (EF) and pair-fed (PF) mice of each sex. Partial least-squares discriminant analysis revealed that ethanol altered colonic lipid composition largely in male mice compared with female mice. A significant increase in free fatty acids, ceramides, and hexosylceramides and decreased phosphatidylglycerols (PG) was observed in the EF group compared to the PF group in male mice. Phosphatidylethanolamine (PE) levels were increased significantly in the EF group of both sexes compared to the PF group. The volcanic plot shows that PG (O-15:1/15:0) and PE (O-18:2/15:0) are common markers that are increased in both sexes of the EF group. In addition, decreased fatty acid esters of hydroxy fatty acids (FAHFA) were observed specifically in the EF group of female mice. Overall, a significant variation in the mice colonic content lipidome between the EF and PF groups was observed. Target pathways, such as sphingolipid metabolism in males, FAHFA in females, and PE metabolism in both sexes, were suggested. This study provides new insight into the sex-dependent lipid change associated with alcohol-induced gut-microbiota dysfunction and its potential health impacts.

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Section: Methodsmentioning

confidence: 99%

Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS

Jayaprakash,

B. Gowda,

K. Shukla

et al. 2024

ACS Omega

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“…[ 39–41 ] Neutral lipids in macroalgae include TAG, [ 37,42 ] diacylglycerides, [ 37 ] and sterols, such as cholesterol, campesterol, and fucosterol. [ 28,43 ] TAG are the most reported in macroalgae, as lipid reservoirs to store cellular carbon and energy. [ 37,44 ]…”

Section: Polar Lipids In Macroalgaementioning

confidence: 99%

“…[39][40][41] Neutral lipids in macroalgae include TAG, [37,42] diacylglycerides, [37] and sterols, such as cholesterol, campesterol, and fucosterol. [28,43] TAG are the most reported in macroalgae, as lipid reservoirs to store cellular carbon and energy. [37,44] Polar lipids are quite abundant lipid group in several macroalga species [28,38,45,46] and so this review is particularly focused on this group.…”

Section: Polar Lipids In Macroalgaementioning

confidence: 99%

“…[78,79] Other classes of lipids, such as PE, PI, PA, SM, and Cer, were identified in macroalgae species from the three different phyla with content and composition variable. [19,43,65] At lipid species level, the lipidomics studies on macroalgae have disclosed a great diversity of composition. An accurate identification at molecular lipid species level through the interpretation of the MS/MS spec-Table 1.…”

Section: Polar Lipidome Profile Of Macroalgae: From Phylum To Species...mentioning

confidence: 99%

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Mapping the Polar Lipidome of Macroalgae using LC‐MS‐Based Approaches for Add‐Value Applications

Lopes

Rey

Melo

et al. 2023

Euro J Lipid Sci & Tech

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Marine macroalgae are emerging as sustainable and healthy food, and a source of lipids with nutritional and bioactive properties. Nonetheless, the lipidome of macroalgae is little explored compared to other photosynthetic organisms. Most of the literature is devoted to the analysis of fatty acid (FA) from macroalgae, because of they are a source of long-chain omega-3 and omega-6 polyunsaturated FA, which can be used as an alternative to fish oil. However, FA occurs mostly esterified in other lipids, such as polar lipids, that still overlooked. The data published on the study of polar lipids (phospholipids, glycolipids, betaine lipids) pinpoint their remarkable characteristics and bioactive properties which mainly remain unexplored. The lipidome of macroalgae seems to be species-specific, while some lipid classes appear to be characteristic of phyla. Lipidome plasticity of macroalgae is verified to change under abiotic and biotic factors which may modulate the functionality and add-value of the biomass. This biochemical plasticity represents an advantage to obtain ingredients for target applications and niche of markets. This review provides an overview on macroalgae lipidome characterization, addressing analysis workflow based on liquid chromatography coupled to mass spectrometry, and discussing the bioactive properties and biotechnological applications of macroalga lipids. Practical Applications: The current state of the art on marine macroalgae lipidomics is reviewed, including the lipid characterization using modern liquid chromatography-mass spectrometry, the distribution of polar lipids by macroalgae species and phylum, their bioactive properties, and potential biotechnological applications. In a time of increasing awareness of sustainably produced marine resources, the lipids from macroalgae are still underexplored. This review provides information for smart valorization of macroalgae as source of add-value lipids contributing to the development of blue bioeconomy.

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“…Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers increased throughput and confidence for lipidomics profiling studies. As a rule, high-resolution MS is the first option for lipidomic analysis, including analysers such as quadrupole-time-of-flight (Q-TOF), Orbitrap, and Fourier transform ion cyclotron resonance mass spectrometers [ 6 , 7 , 8 ]. Many LC configurations have been described for the chromatographic separation of neutral, semi-polar, and polar lipids from complex mixtures such as reversed phase (RP), normal phase (NP), and hydrophilic interaction chromatography (HILIC).…”

Section: Introductionmentioning

confidence: 99%

Untargeted UHPLC-TOF/MS Lipidomic Analysis for the Investigation of Egg Yolks after Xylanase Supplementation of the Diet of Laying Hens

et al. 2023

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Xylanase supplementation of diets is used to enhance nutrient digestibility in monogastrics which lack necessary enzymes for non-starch polysaccharide degradation. The effects of enzymatic treatment in the nutritional value of the feed are typically not comprehensively studied. Though the fundamental effects of xylanase on performance are well studied, limited data is available on the complex interactions between xylanase supplementation and hen physiology; therefore, the aim of this study was to develop a new, simple UPLC-TOF/MS lipidomics method for the analysis of hen egg yolks after supplementation with different amounts of xylanase. Sample preparation for the extraction of lipids was optimized and different sample preparation modes and solvent mixtures were tested. Optimal results for the extraction of total lipids were obtained by using the solvent mixture MTBE: MeOH (5:1, v/v). Multivariate statistical analysis of the signals of hundreds of lipids in positive and negative ionisation modes highlighted differences in several egg yolk lipid species-classes. Four lipid species-classes, phosphatidylcholines (PC and PC O), phosphatidylethanolamines (PE and PE O), phosphatidylinositols (PI), and fatty acids (FA), were among those contributing to the separation of the experimental groups (control-treated) in negative ionisation mode. In positive ionisation mode, principal beneficial lipid compounds such as phosphatidylcholines (PC and PC O), phosphatidylethanolamines (PE and PE O), triacylglycerols (TG), diacylglycerols (DG), and ceramides (Cer) were found to be increased in treated groups. Overall, supplementation of laying hens’ diets with xylanase significantly changed the lipid profile of egg yolks compared to the control diet. The association between the lipid profiles of egg yolks and hens’ diets, as well as the underlying mechanisms, require further investigation. These findings are of practical significance for the food industry.

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Analysis of Antioxidant Lipids in Five Species of Dietary Seaweeds by Liquid Chromatography/Mass Spectrometry

Cited by 10 publications

References 56 publications

Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS

Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS

Mapping the Polar Lipidome of Macroalgae using LC‐MS‐Based Approaches for Add‐Value Applications

Untargeted UHPLC-TOF/MS Lipidomic Analysis for the Investigation of Egg Yolks after Xylanase Supplementation of the Diet of Laying Hens

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