Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection

Derksen, G.C.H.; Niederländer, H.A.G.; Beek, T.A. van

doi:10.1016/s0021-9673(02)01412-7

Cited by 109 publications

(62 citation statements)

References 22 publications

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“…In this study, as well as in previous reports [38,39], the analysis of anthraquinones using online mass spectrometric detection was found to be less sensitive than on-line UV detection. Therefore, HPLC-UV was chosen for the determination of compounds 1-6.…”

Section: Calibration Curvessupporting

confidence: 70%

“…Several analytical methods such as thin-layer chromatography [28], micellar electrokinetic capillary chromatography [29], capillary zone electrophoresis [30], capillary electrophoresis-mass spectrometry (CE-MS) [31], gas chromatography [32], and gas chromatography-mass spectrometry (GC-MS) [33] have been used for the determination of anthraquinones in various medicinal plants and natural products. In addition, analytical methods involving the use of HPLC [32][33][34][35][36] and liquid chromatographymass spectrometry (LC-MS) [37][38][39][40] have also been used for anthraquinone analysis in several natural products.…”

Section: Published In Final Edited Form Asmentioning

confidence: 99%

“…Generally, the use of LC-MS is advantageous over the use of CE-MS and GC-MS because it affords high separation efficiency and accurate identification of analytes over a wide range of molecular structures, chemical reactivities, polarities, and volatilities. Hence, separation of analytes as well as mass identification and confirmation of analytes can be achieved simultaneously in one experimental run using LC-MS. For example, LC-MS using electrospray ionization (ESI) [37,38] and atmospheric pressure chemical ionization (APCI) [39,40] has been used for accurate identification of anthraquinones in plant extracts. In comparison with APCI-MS detection, the use of ESI-MS detection for anthraquinone identification is less effective, because the latter requires mobile phase additives other than the buffer to enhance analyte ionization [37,38].…”

Section: Published In Final Edited Form Asmentioning

confidence: 99%

“…In comparison with APCI-MS detection, the use of ESI-MS detection for anthraquinone identification is less effective, because the latter requires mobile phase additives other than the buffer to enhance analyte ionization [37,38]. Moreover, aside from eluent additives, the efficiency of the mass detection of anthraquinones using an ESI interface depends on the pK a value of the analyte [38].…”

Section: Published In Final Edited Form Asmentioning

confidence: 99%

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Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography

Fernand

Dinh

Washington

et al. 2008

Talanta

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A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of six phenolic compounds, five anthraquinones (rhein, aloe-emodin, emodin, chrysophanol and physcion) and a flavonoid (kaempferol), in root extracts from Cassia alata L. Solid-phase extraction, using C 18 cartridges, was used to remove interfering substances from the root extracts. The extracts were analyzed on a C 18 column using an isocratic mobile phase which consisted of acetonitrile, methanol, and 10 mM aqueous ammonium acetate (25: 55: 20, v/v). Identification of the analytes was performed by use of standards and on-line mass spectrometric detection using atmospheric pressure chemical ionization. The concentration of the phenolic compounds in the root extracts was determined using HPLC with ultraviolet detection at 260 nm. The limits of detection obtained for the anlytes were in the range of 0.23 -4.61 ppm. The overall R.S.D. precision values (intra and inter-day) for the retention times and peak-areas were lower than 0.16% and 2.10%, respectively. In addition, the recovery of the developed method for the analysis of these phenolic compounds was determined, and ranged from 81.2 ± 4.3% to 106 ± 2%.

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Section: Calibration Curvessupporting

confidence: 70%

Section: Published In Final Edited Form Asmentioning

confidence: 99%

Section: Published In Final Edited Form Asmentioning

confidence: 99%

Section: Published In Final Edited Form Asmentioning

confidence: 99%

See 2 more Smart Citations

Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography

Fernand

Dinh

Washington

et al. 2008

Talanta

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show abstract

“…acid digestion, 18 spectrophotometry in the ultraviolet, visible and infrared regions 17 and liquid chromatography 19,23,24,29 -31 (especially useful in such analyses is UV/visible diode-array detection (DAD)). For the identification of isomers of laccaic acids 32 in lac-dye and alizarin and purpurin in madder, 26 the liquid chromatography/mass spectrometry (LC/MS) has been proposed. Coupled methods 33,34 have recently been found to be extremely efficient in complicated analyses of very small samples.…”

Section: Introductionmentioning

confidence: 99%

Identification of anthraquinone coloring matters in natural red dyes by electrospray mass spectrometry coupled to capillary electrophoresis

et al. 2003

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Capillary electrophoresis with UV/visible diode-array detection (DAD) and electrospray mass spectrometric (ESI-MS) detection were used for the identification of anthraquinone color components of cochineal, lac-dye and madder, natural red dyestuffs often used by ancient painters. For the purpose of such analysis, ESI-MS was found to be a much more appropriate detection technique than DAD one owing to its higher sensitivity (detection limits in the range 0.1-0.5 micro g ml(-1)) and selectivity. The method developed made it possible to identify unequivocally carminic acid and laccaic acids A, B and E as coloring matters in the examined preparations of cochineal and lac-dye, respectively. In madder, European Rubia tinctorum, alizarin and purpurin were found. The method allows the rapid, direct and straightforward identification and quantification of components of natural products used in art and could be very helpful in restoration and conservation procedures.

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Characterization of Organic Natural Dyes by Electrospray Mass Spectrometry Coupled with HPLC and/or Capillary Electrophoresis

Lech

Pawlak

Jarosz

2009

Organic Mass Spectrometry in Art and Archaeology

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Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection

Cited by 109 publications

References 22 publications

Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography

Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography

Identification of anthraquinone coloring matters in natural red dyes by electrospray mass spectrometry coupled to capillary electrophoresis

Characterization of Organic Natural Dyes by Electrospray Mass Spectrometry Coupled with HPLC and/or Capillary Electrophoresis

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