Analysis of Anthelmintic and Anticoccidial Drug Residues in Animal&amp;;#x02010;Derived Foods

Tuck, Sarah; Furey, Ambrose; Danaher, Martin

doi:10.1002/9781118696781.ch5

Cited by 6 publications

(5 citation statements)

References 127 publications

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“…Some commonly found anticoccidial drugs in food include salinomycin, narasin, monensin, toltrazuril, robenidine, nicarbazin, dinitrocarbanilide, dimetridazole, decoquinate and lasalocid [ 26 , 28 ]. Anticoccidial residue in food exhibits undesirable toxicological effects, such as teratogenicity, hepatotoxicity or neurotoxicity, at high doses in laboratory animals [ 29 ].…”

Section: Drug Residues In Foodmentioning

confidence: 99%

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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The presence of drug residues in food products has become a growing concern because of the adverse health risks and regulatory implications. Drug residues in food refer to the presence of pharmaceutical compounds or their metabolites in products such as meat, fish, eggs, poultry and ready-to-eat foods, which are intended for human consumption. These residues can come from the use of drugs in the field of veterinary medicine, such as antibiotics, antiparasitic agents, growth promoters and other veterinary drugs given to livestock and aquaculture with the aim of providing them as prophylaxis, therapy and for promoting growth. Various analytical techniques are used for this purpose to control the maximum residue limit. Compliance with the maximum residue limit is very important for food manufacturers according to the Food and Drug Administration (FDA) or European Union (EU) regulations. Effective monitoring and control of drug residues in food requires continuous advances in analytical techniques. Few studies have been reviewed on sample extraction and preparation techniques as well as challenges and future directions for the determination of veterinary drug residues in food. This current review focuses on the overview of regulations, classifications and types of food, as well as the latest analytical methods that have been used in recent years (2020–2023) for the determination of drug residues in food so that appropriate methods and accurate results can be used. The results show that chromatography is still a widely used technique for the determination of drug residue in food. Other approaches have been developed including immunoassay, biosensors, electrophoresis and molecular-based methods. This review provides a new development method that has been used to control veterinary drug residue limit in food.

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Section: Drug Residues In Foodmentioning

confidence: 99%

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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“…The widespread use of different coccidiostats results in a considerable risk of their residues remaining in edible tissues. Coccidiostats residues in food exhibit undesirable toxicological effects, such as teratogenicity, hepatotoxicity, or neurotoxicity, at high doses in laboratory animals [ 10 , 11 ]. Carboxylic acid ionophores are potent pharmacological agents, exerting marked cardiovascular effects in experimental animal systems.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Screening of Salinomycin in Feed and Its Residues in Poultry Tissues Using Microbial Inhibition Tests Coupled to Enzyme-Linked Immunosorbent Assay (ELISA)

Spišáková,

Kožárová,

Hriciková

et al. 2024

Foods

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Salinomycin is a coccidiostat approved as a feed additive for the prevention of coccidiosis in poultry. Official control of its residues is set by the Commission Delegated Regulation (EU) 2022/1644. The aim of our study was to assess the suitability of three microbial inhibition tests (MITs), Premi®Test, Explorer 2.0, and the Screening Test for Antibiotic Residues (STAR) linked to the enzyme-linked immunosorbent assay (ELISA), for the screening of salinomycin residues in the tissues of broiler chickens (breast and thigh muscle, heart, liver, gizzard, kidneys, lungs, spleen, skin, and fat) fed commercially produced feed containing 70 mg.kg−1 of salinomycin in the complete feed. The first residue screening (Sampling A) was performed on the last day of administration of the salinomycin-medicated feed (day 30), and the second screening (Sampling B) was performed on the day of slaughter (day 37) after the expiry of the withdrawal period with the feeding of non-medicated feed. Based on the quantitative confirmation of salinomycin residues in the examined chicken tissues by the ELISA method (Sampling A from 0.025 to 0.241 mg kg−1; Sampling B from 0.003 to 0.076 mg kg−1), all the MITs with the preference of the bacterial strain Bacillus stearothermophilus var. calidolactis ATCC 10149 demonstrated the ability to detect the residues of salinomycin in the examined tissues of broiler chickens at the level of maximum residue limits set from 0.15 to 0.150 mg kg−1 by the Commission Implementing Regulation (EU) 2017/1914 and confirmed the relevancy of their sensitivity to coccidiostat salinomycin.

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“…Long-term drug administration is the only method to prevent and control coccidiosis in farm animals ( 3 – 5 ). Diclazuril, a triazine acetonitrile compound, is a non-polyether synthetic anti-coccidial drug that was developed by Janssen in the 1980s ( 6 , 7 ). It is a highly efficient, low toxic, and broad-spectrum anti-coccidiosis drug.…”

Section: Introductionmentioning

confidence: 99%

Generation and Characterization of an Anti-diclazuril Monoclonal Antibody and Development of a Diagnostic Enzyme-Linked Immunosorbent Assay for Poultry

Shen

Sun

et al. 2022

Front. Nutr.

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An anti-diclazuril monoclonal antibody (mAb) was developed for use in enzyme-linked immunosorbent assay (ELISA)-based detection of diclazuril with high sensitivity and specificity, which can be used to measure anti-coccidial drug residues. The anti-diclazuril mAb had a half-maximal inhibitory concentration of 0.449–0.517 ng/mL. The mAb cross-reactivity with toltrazuril, toltrazuril 18 sulfone, clozaril, monesin, madurmycin, and salinomycin was very minimal (< 0.1%). The detection limit of the ELISA using this mAb was 0.10 ng/mL and the sensitivity was 0.05 ng/mL. A standard curve generated in the range of 0.05–16.2 ng/mL had a linear correlation coefficient value of ≥ 0.99. The average recoveries of diclazuril from chicken and duck samples ranged from 85.0 to 102.5%.Intra- and inter-assay coefficients of variation ranged from 5.9 to 8.5% and 9.2 to 12.6%, respectively. Using the International Immunogenetics Information System®, the VH domain of the mAb was found to be encoded by an IGHV3 family gene and had the following complementarity determining region (CDR) sequences: GFTFSRY (CDR1), SRGGS (CDR2), and GDDNYAFAY (CDR3). The VL domain was encoded by an IGKV1 family gene and had the following CDR sequences: KSSQSLLNSRTRKNYLA (CDR1), WASTRES (CDR2), and KQSYNLHT (CDR3). This study provides a method to generate anti-diclazuril mAbs and determine their variable region sequences. The diagnostic ELISA developed using this mAb may drive additional studies on the monitoring and detection of food and veterinary drug residues.

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Analysis of Anthelmintic and Anticoccidial Drug Residues in Animal&;#x02010;Derived Foods

Cited by 6 publications

References 127 publications

Recent Advances in the Determination of Veterinary Drug Residues in Food

Recent Advances in the Determination of Veterinary Drug Residues in Food

Comprehensive Screening of Salinomycin in Feed and Its Residues in Poultry Tissues Using Microbial Inhibition Tests Coupled to Enzyme-Linked Immunosorbent Assay (ELISA)

Generation and Characterization of an Anti-diclazuril Monoclonal Antibody and Development of a Diagnostic Enzyme-Linked Immunosorbent Assay for Poultry

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