Analysis of Angiotensin I Converting Enzyme Inhibitory Activity of Oligosacchride Extracted from Capsosiphon fulvescens

The aim of this study was to examine inhibitory effects of pine needle ethanol extracts (PNEE) on atopic dermatitis (AD). To determine inflammatory activity PNEE was added to LPS-induced murine peritoneal macrophages for an in-vitro test. In addition, anti-AD test was carried out by spreading PNEE on the dorsal skin of 2,4-dinitrochlorobenzene (DNCB)-induced BALB/c mice. It was confirmed that the nitric oxide (NO) secretion was suppressed when 15 0 µg/mL of PNEE were added to LPS-induced murine peritoneal macrophages. Moreover, levels of TNF-α, IL-6, and IL-1β‚ were decreased. For the anti-AD test, PNEE alleviated symptoms of the erythema in DNCB-induced mice. Furthermore, the IFN-γ secretion of the group treated with PNEE was increased in splenocytes from DNCB-induced mice compared to the positive control, while IL-4 secretion diminished. Through these results, we can conclude that PNEE can inhibit AD by modulating the IFN-γ, IL-4 cytokines production and inhibiting inflammation.

Effect of Pine needle Ethanol Extracts on the Inhibitory Activity of Atopic Dermatitis

Jeong¹,

Kim²,

Jung³

et al. 2013

Analysis of Microbial Community Structure in Biological Wastewater Treatment Process of Mixed Wastewater Treatment Facility using Environmental·Ecological Technique

손형식¹,

Joon²,

Son³

2013

The bacterial community structure in a biological reactor fed influent from a wastewater treatment system was investigated by denaturing gradient gel electrophoresis (DGGE) and in situ hybridization. Sludges were collected from three biological reactors (aerobic,oxic,and anoxic tanks) at the M wastewater treatment facility (WTF). The influent of the MWTF consisted of mixed tannery wastewater (40~65%) and seafood wastewater (35~60%). The treatment processes resulted in a removal efficiency for BOD (biochemical oxygen demand) and COD (chemical oxygen demand) of 83.69 8.2% and 72.8~84.6%, respectively for tannery wastewater than for seafood wastewater resulted in greater survival of biomass in the biological reactors and a higher removal of BOD, COD, and T-N of about 8~18%. In contrast, addition of greater amounts of seafood wastewater decreased the amount of biomass in the bioreactors due to the increasing concentration of chromium from that wastewater and it also. The dominant bacterial species during the high seafood wastewater input period were Burkholderia cepacia (JX901049) and an uncultured bacterium (JF247555), while Pseudomonas geniculata (HQ256559) was dominant during the high tannery wastewater input period. Flavobacteriumsp. BF.107 (FM173271) and Hyphomicrobium zavarzinii (Y14306) were dominant under anoxic conditions.

Effects of Silkworm Gland Hydrolysate on Albumin-erythropoietin Production in Transgenic Chinese Hamster Ovary Cells

최민호¹,

차현명²,

김선미³

et al. 2013

To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26-fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.