Analysis of an expanded width of albumin fraction by cellulose acetate membrane electrophoresis in IgA nephropathy urine before treatment

Abstract

Cellulose acetate membrane electrophoresis with colloidal silver stain re-vealed that the width of the albumin fraction in IgA nephropathy (IgAN) urine before treatment was significantly expanded. This phenomenon was not shown in IgAN urine after treatment or in non-IgAN urine. There was a reverse correlation between the width of the albumin fraction and the albumin con-centration in IgAN urine. By immuno-fixation, Tamm-Horsfall protein (THP) was located in the same position as the albumin band in IgAN urine before treatment; however, in the urine of a healthy subject it was located in the same position as alpha(1)-globulin. By ELISA, the THP-albumin complex concentration in IgAN urine before treatment was significantly higher than in the other two diseases. The width of the albumin fraction and the sodium ion concentra-tion of the urine were significantly correlated. The THP/albumin ratio in IgAN urine before treatment was significantly higher than in the other two groups. This suggests that the characteristic expanded width of albumin found by immunofixation indicates a THP-albumin complex, and that the sodium concentration of urine is involved in the formation of this complex.

The complex of immunoglobulin A and uromodulin as a diagnostic marker for immunoglobulin A nephropathy

“…The urine of IgAN is known to have a rather high concentration of the albumin–uromodulin complex [ 23 ]. The IgA–uromodulin complex might include IgA–uromodulin–albumin complex, but this three-component complex is considered to be a minor component, because the concentration of the IgA−albumin complex was even lower than that of the IgA–uromodulin complex (data not shown).…”

Cellulose Acetate Membrane Electrophoresis Based Urinary Proteomics for the Identification of Characteristic Proteins

“…Different profiles are often seen in the CAME of urinary proteins from patients with renal diseases . To identify the proteins on CAME, immune fixation and Western blot have been employed using specific antibodies ; however, the protein contents in each fraction have not been fully identified.…”

Urinary protein fraction in healthy subjects using cellulose acetate membrane electrophoresis followed by colloidal silver staining

“…With this method, it is possible to detect specific abnormalities in urinary proteins, such as abnormalities in the pattern of urinary protein fractions on CAE. In previous studies, we found that some specific patterns of urinary protein fractions appear in various renal disorders (3)(4)(5). The detection of Bence-Jones protein (BJP), which appears in the urine of patients with multiple myeloma, malignant monoclonal gammopathy, and lymphoproliferative disease, is particularly useful clinically.…”