Analysis of amphetamines and metabolites in urine with ultra performance liquid chromatography tandem mass spectrometry

Fernández, María del Mar Ramírez; Wille, Sarah M. R.; Fazio, Vincent Di; Gosselin, Matthias; Samyn, Nele

doi:10.1016/j.jchromb.2010.03.048

Cited by 24 publications

(7 citation statements)

References 17 publications

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“…Several works have compared HPLC to UHPLC applications. Most of them have reported the major advantage of UHPLC over HPLC to be the overall spent time, under isocratic or gradient elution . In this fashion, an UHPLC system coupled with mass spectrometer analyzers become a powerful tool for the fast analysis of small molecules within complex matrices, with lower quantification and detection limits.…”

Section: Resultsmentioning

confidence: 99%

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Quantification of ampicillin in bovine milk by coupled‐column ultrahigh‐performance liquid chromatography‐tandem mass spectrometry

Moura

Almeida²,

Lopes³

et al. 2012

J of Separation Science

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This work reports the use of two-dimensional (2D) liquid chromatography system coupled with a tandem mass spectrometry for the quantification of ampicillin in bovine milk. A restrict access media column (RAM-BSA C(8) , 50 × 2.1 mm, Luna, 10 μm, 100 Å) was used in the first dimension in order to exclude macromolecules, while an ACQUITY UPLC BEH C(18) (50 × 2.1 mm, 1.7 μm) column was used in the second dimension. Three different channels of selected reaction monitoring (SRM) were used: 350 > 106 m/z, 350 > 160 m/z, and 350 > 192 m/z. The first transition was used for the quantification (higher intensity), and latter two for confirmation. The developed method is simple and requires a total analysis time of only 14 min/sample. The sample treatment involved only a centrifugation step for 20 min. The validated method has been successfully applied to monitor AMP residues in raw milk samples. To our knowledge, this is the first study to report the use of ultrahigh-performance liquid chromatography (UHPLC) in 2D configuration.

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Section: Resultsmentioning

confidence: 99%

“…This has stimulated the use of UHPLC‐MS/MS for analysis of drugs in biological samples. However, the procedures used for sample cleanup remain laborious, such as off‐line solid phase extraction (SPE), protein precipitation (PP), and liquid–liquid extraction (LLE) .…”

Section: Introductionmentioning

confidence: 99%

Quantification of ampicillin in bovine milk by coupled‐column ultrahigh‐performance liquid chromatography‐tandem mass spectrometry

Moura

Almeida²,

Lopes³

et al. 2012

J of Separation Science

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show abstract

“…In general, blood samples were collected in venutubes with sodium fluoride as the anticoagulant. STA, applied in this study, consisted of screening using high-resolution mass spectrometry (LC-HRMS) via the Xevo-G2-QTOF-XS (Waters, Manchester, UK) with UNIFI software [23] followed by several target drug methods [24][25][26][27][28][29][30][31][32] applied for confirmation and quantification [25][26][27][28][29][30][31][32][33]. A total sample volume of maximum 1 mL (100 µL for screening and 900 µL for all confirmation and quantification methods) was necessary when applying the methods as stated below.…”

Section: Stamentioning

confidence: 99%

The Interest of a Systematic Toxicological Analysis Combined with Forensic Advice to Improve the Judicial Investigation and Final Judgment in Drug Facilitated Sexual Assault Cases

et al. 2021

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The conviction rate in drug facilitated sexual assault (DFSA) cases is known to be very low. In addition, the potential impact of toxicological results on the case is often not well understood by the judicial authorities. The aims of this study were (1) to obtain more knowledge concerning the prevalence of incapacitating substances in DFSA cases, (2) to create a more efficient DFSA analysis strategy taking background information into account, and (3) to evaluate the potential impact of systematic toxicological analysis (STA) on the final judicial outcome. This small-scale epidemiological study (n = 79) demonstrates that ‘commonly-used’ illicit drugs, psychoactive medicines and ethanol are more prevalent in DFSA cases in contrast to the highly mediatized date rape drugs. Additionally, via case examples, the interest of performing STA—to prove incapacitation of the victim—in judicial procedures with mutual-consent discussions has been demonstrated as it led to increased convictions. However, more attention has to be paid to ensure a short sampling delay and to get more accurate information from the medical treatment of the alleged victim. This will improve the interpretation of the toxicological analysis and thus its applicability in a DFSA case. The future is multi-disciplinary and will certainly lead to an efficient and more cost-effective DFSA approach in which STA can impact the final judgment.

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“…[4][5][6][7][8][9][10][11][12] The applied method to detect benzodiazepines and their metabolites as well as several hypnotics in blood and urine is described by our research group. [4][5][6][7][8][9][10][11][12] The applied method to detect benzodiazepines and their metabolites as well as several hypnotics in blood and urine is described by our research group.…”

Section: Target Techniquesmentioning

confidence: 99%

“…The methods used for target quantification in urine and blood are described in the literature. [4][5][6][7][8][9][10][11][12] The applied method to detect benzodiazepines and their metabolites as well as several hypnotics in blood and urine is described by our research group. [7] However, the HPLC method was transferred to a UPLC method.…”

Section: Target Techniquesmentioning

confidence: 99%

Drug‐facilitated sexual crime by use of ketamine and diazepam by a gynaecologist

Wille

Fazio

Samyn

2013

Drug Testing and Analysis

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Analysis of amphetamines and metabolites in urine with ultra performance liquid chromatography tandem mass spectrometry

Cited by 24 publications

References 17 publications

Quantification of ampicillin in bovine milk by coupled‐column ultrahigh‐performance liquid chromatography‐tandem mass spectrometry

Quantification of ampicillin in bovine milk by coupled‐column ultrahigh‐performance liquid chromatography‐tandem mass spectrometry

The Interest of a Systematic Toxicological Analysis Combined with Forensic Advice to Improve the Judicial Investigation and Final Judgment in Drug Facilitated Sexual Assault Cases

Drug‐facilitated sexual crime by use of ketamine and diazepam by a gynaecologist

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