Analysis of Amisulpride in Human Plasma by SPE and LC with Fluorescence Detection

Kudris, Illia V.; Skakun, Nona N.; Orlova, I. N.; Libina, Victoria V.; Kulikov, A. Yu.

doi:10.1007/s10337-010-1832-2

Cited by 21 publications

(11 citation statements)

References 14 publications

(23 reference statements)

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“…63,64 Recently, determination of amisulpride by LC-MS/MS with positive electrospray ionization has gained importance. 63,64 Recently, determination of amisulpride by LC-MS/MS with positive electrospray ionization has gained importance.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Drug Monitoring of Common Antipsychotics

Patteet

Morrens

Maudens

et al. 2012

Therapeutic Drug Monitoring

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The aim of this review is to provide information for interpreting outcome results from monitoring of antipsychotics in biological samples. A brief overview of the working mechanisms, pharmacological effects, drug interactions, and analytical methods of classical and atypical antipsychotics is given. Nineteen antipsychotics were selected based on their importance in the worldwide market as follows: amisulpride, aripiprazole, asenapine, bromperidol, clozapine, flupenthixol, haloperidol, iloperidone, lurasidone, olanzapine, paliperidone, perphenazine, pimozide, pipamperone, quetiapine, risperidone, sertindole, sulpiride, and zuclopenthixol. A straightforward relationship between administered dose, plasma or serum concentration, clinical outcome, or adverse effects is often lacking. Nowadays, focus lies on therapeutic drug monitoring and individualized therapy to find adequate treatment, to explain treatment failure or nonresponse, and to check patient compliance. However, extensive research in this field is still mandatory.

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Section: Methodsmentioning

confidence: 99%

Therapeutic Drug Monitoring of Common Antipsychotics

Patteet

Morrens

Maudens

et al. 2012

Therapeutic Drug Monitoring

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“…Amisulpride was quantified as described by Kudris et al with modifications (30). A LaChrom system (Merck Hitachi, Darmstadt, Germany) was used with an interface (D-7000, Merck Hitachi), a pump (L-7100, Merck Hitachi), an autosampler (L-7200, Merck Hitachi), a fluorescence detector (L-7400, Merck Hitachi), and a degasser (L-7614, Merck Hitachi).…”

Section: Hplc Quantification Of Amisulpridementioning

confidence: 99%

The Poorly Membrane Permeable Antipsychotic Drugs Amisulpride and Sulpiride Are Substrates of the Organic Cation Transporters from the SLC22 Family

Pereira

Tadjerpisheh

Abed

et al. 2014

AAPS J

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Abstract. Variations in influx transport at the blood-brain barrier might affect the concentration of psychotropic drugs at their site of action and as a consequence might alter therapy response. Furthermore, influx transporters in organs such as the gut, liver and kidney may influence absorption, distribution, and elimination. Here, we analyzed 30 commonly used psychotropic drugs using a parallel artificial membrane permeability assay. Amisulpride and sulpiride showed the lowest membrane permeability (P e <1.5×10−6 cm/s) and will require influx transport to penetrate the blood-brain barrier and other physiological barriers. We then studied the uptake of amisulpride and sulpiride by the organic cation transporters of the SLC22 family OCT1, OCT2, OCT3, OCTN1, and OCTN2 Amisulpride was found to be transported by all five transporters studied. In contrast, sulpiride was only transported by OCT1 and OCT2. OCT1 showed the highest transport ability both for amisulpride (CL int =1.9 ml/min/mg protein) and sulpiride (CL int =4.2 ml/min/mg protein) and polymorphisms in OCT1 significantly reduced the uptake of both drugs. Furthermore, we observed carrier-mediated uptake that was inhibitable by known OCT inhibitors in the immortalized human brain microvascular endothelial cell line hCMEC/D3. In conclusion, this study demonstrates that amisulpride and sulpiride are substrates of organic cation transporters of the SLC22 family. SLC22 transporters may play an important role in the distribution of amisulpride and sulpiride, including their ability to penetrate the blood-brain barrier.

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“…Protein precipitation is quick and easy, but it did not result in a very clean final extract and caused ion suppression in LC‐MS/MS analysis. Solid‐phase extraction is viewed by some researchers as an efficient mean of sample preparation that leads to minimal matrix effect of biological samples (Kudris et al , ; Wong et al , ), but its reproducibility was not acceptable. Finally, considering the chemical properties of NPD, LLE was used as the preparation technique for effective sample clean‐up and high recovery.…”

Section: Discussionmentioning

confidence: 99%

Tissue distribution and excretion study of neopanaxadiol in rats by ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Geng

Yin

et al. 2014

Biomedical Chromatography

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Neopanaxadiol (NPD), a major ginsenoside in Panax ginseng C. A. Meyer (Araliaceae), was reported to have neuroprotective effect. In this study, a method of ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/QTOF-MS) was developed and validated for quantitative analysis of NPD in tissues, urine and feces, using liquid-liquid extraction (LLE) to isolate NPD from different biological samples, and chromatographic separation was performed on an Agilent Zorbax Stable Bond C18 (2.1 × 50 mm, 1.8 µm) column with 0.1% formic acid in water and acetonitrile. All standard calibration curves were linear (all r(2) > 0.995) within the test range. After oral administration, NPD was extensively distributed to most of the tissues without long-term accumulation. The higher levels were observed in stomach and intestine, followed by kidney and liver. Approximately 64.56 ± 20.32% of administered dose in feces and 0.0233 ± 0.0356% in urine were found within 96 h, which indicated that the major elimination route was fecal excretion. This analytical method was applied to the study of NPD distribution and excretion in rats after oral intake for the first time. The results we found here are helpful for us to understand the pharmacological effects of NPD, as well as its toxicity.

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Analysis of Amisulpride in Human Plasma by SPE and LC with Fluorescence Detection

Cited by 21 publications

References 14 publications

Therapeutic Drug Monitoring of Common Antipsychotics

Therapeutic Drug Monitoring of Common Antipsychotics

The Poorly Membrane Permeable Antipsychotic Drugs Amisulpride and Sulpiride Are Substrates of the Organic Cation Transporters from the SLC22 Family

Tissue distribution and excretion study of neopanaxadiol in rats by ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

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