Analysis of altered microRNA expression profile in the reparative interface of the femoral head with osteonecrosis

Hou, Yuan; Roemeling, Christina A. Von; Gao, Hui-di; Zhang, Jing; Guo, Changan; Yan, Zuoqin

doi:10.1016/j.yexmp.2015.01.002

Cited by 28 publications

(28 citation statements)

References 31 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current study also provides an explanation for why mir‐34a was up‐regulated in the bone samples of necrotic femoral heads in Yuan's study . In this study, the samples used for analysis were derived from reparative regions that consist of disordered and thickened newly formed osteocytes, not the necrotic areas.…”

Section: Discussionmentioning

confidence: 79%

“…A number of miRNAs, such as mir‐17/20a and mir‐29a, have been recognized as playing a functional role in glucocorticoid‐induced bone loss as well as necrosis. In a recent study, we determined which miRNAs are abnormal in patients with GIOFH and femoral neck fractures using miRNA microarray assays, and the results showed significant upregulation of the expression of 12 miRNAs (including mir‐34a), while 5 other miRNAs were down‐regulated . Based on previous studies, we know that mir‐34a possesses many functions, mainly acting within various types of tumors.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulatory effect of microRNA‐34a on osteogenesis and angiogenesis in glucocorticoid‐induced osteonecrosis of the femoral head

Zha

Sun

Wei

et al. 2017

Journal Orthopaedic Research

View full text Add to dashboard Cite

Glucocorticoid-induced osteonecrosis of the femoral head (GIOFH) is a common and devastating orthopedic disease, and its underlying mechanism remains unclear. The aim of this study was to determine the role of microRNA-34a (mir-34a) in GIOFH. C57 mouse mesenchymal stem cells (mMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured with dexamethasone (Dex). A total of 48 adult rats were treated with glucocorticoids, and after the onset of GIOFH, each femoral head was removed. Mir-34a mimics, an inhibitor and over-expressing lentivirus were used in vitro and in vivo, respectively. Real-time PCR, immunohistochemistry, ELISA, cell proliferation assays, osteoblastic differentiation, and endothelial activity assays were employed to evaluate the effect of mir-34a on mMSCs, osteoblasts, and vascular endothelial cells in glucocorticoid-treated mice. We found that Dex inhibited mMSC proliferation and osteoblastic differentiation, as well as the viability and activity of endothelial cells. Dex also caused osteonecrosis and decreased new vessel formation in vivo. Mir-34a alleviated the inhibitory effects of Dex on mMSCs and osteoblasts, while facilitating its inhibitory effects on endothelial cells. Mir-34a is an important regulator in osteogenesis and angiogenesis, and it might be useful as a therapeutic target for GIOFH. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:417-424, 2018.

show abstract

Section: Discussionmentioning

confidence: 79%

mentioning

confidence: 99%

Regulatory effect of microRNA‐34a on osteogenesis and angiogenesis in glucocorticoid‐induced osteonecrosis of the femoral head

Zha

Sun

Wei

et al. 2017

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…Bone healing after fracture is a complicated and sequential process including inflammation, angiogenesis, progenitor cell recruitment, chondrogenesis, osteogenesis, and osteoclastogenesis [19,20,33,34]. In the aged population, the processes of angiogenesis, chondrogenesis, and osteogenesis have been down-regulated [35,36], whereas the process of osteoclastogenesis has been up-regulated [37], which results in impaired fracture healing. However, the regulatory mechanisms underlying these changes still have not been fully understood.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics and Microarray Analysis of miRNAs in Aged Female Mice Model Implied New Molecular Mechanisms for Impaired Fracture Healing

Liu

et al. 2016

IJMS

View full text Add to dashboard Cite

Impaired fracture healing in aged females is still a challenge in clinics. MicroRNAs (miRNAs) play important roles in fracture healing. This study aims to identify the miRNAs that potentially contribute to the impaired fracture healing in aged females. Transverse femoral shaft fractures were created in adult and aged female mice. At post-fracture 0-, 2- and 4-week, the fracture sites were scanned by micro computed tomography to confirm that the fracture healing was impaired in aged female mice and the fracture calluses were collected for miRNA microarray analysis. A total of 53 significantly differentially expressed miRNAs and 5438 miRNA-target gene interactions involved in bone fracture healing were identified. A novel scoring system was designed to analyze the miRNA contribution to impaired fracture healing (RCIFH). Using this method, 11 novel miRNAs were identified to impair fracture healing at 2- or 4-week post-fracture. Thereafter, function analysis of target genes was performed for miRNAs with high RCIFH values. The results showed that high RCIFH miRNAs in aged female mice might impair fracture healing not only by down-regulating angiogenesis-, chondrogenesis-, and osteogenesis-related pathways, but also by up-regulating osteoclastogenesis-related pathway, which implied the essential roles of these high RCIFH miRNAs in impaired fracture healing in aged females, and might promote the discovery of novel therapeutic strategies.

show abstract

“…The procedure for the preparation of samples can be found in our previous report 50 . All samples met the quality control standards.…”

Section: Methodsmentioning

confidence: 99%

Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

Hou

Roemeling

Guo

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Angiogenesis is an important event in steroid-associated osteonecrosis of the femoral head (SONFH). Here we performed miRNA microarray with SONFH tissues (ONs) and the adjacent normal tissues (NLs) to select the angiogenic miRNA. The results showed that miR-210 was differentially expressed in SONFH versus normal tissues. Unexpectedly, its specific transcription factor, hypoxia-inducible factor-1α, was shown of no significant changes in ONs compared with NLs. Further Bisulfite sequencing revealed that miR-210 is embedded in a CpG island and miR-210 gene has 2 CpG sites with lower methylation percentage in ONs compared with NLs. Additionally, ONs with lower miR-210 gene methylation exhibited higher miR-210 expression. Next, we found that the endothelial cells treated with demethylating agents could significantly increase the expression of miR-210, along with promoted cell viability and differentiation. Some angiogenic genes (VEGF, bFGF, TNF-α and PCNA) were up-regulated as well. In addition, the supernatant of the cells after demethylation treatment displayed an enhanced ability of recruiting new microvessels in vivo. Taken together, our study not only provides novel insights into the regulation of angiogenesis in this disease, but also reveals a therapeutic opportunity for treatment of SONFH patients with demethylating agents.

show abstract

Analysis of altered microRNA expression profile in the reparative interface of the femoral head with osteonecrosis

Cited by 28 publications

References 31 publications

Regulatory effect of microRNA‐34a on osteogenesis and angiogenesis in glucocorticoid‐induced osteonecrosis of the femoral head

Regulatory effect of microRNA‐34a on osteogenesis and angiogenesis in glucocorticoid‐induced osteonecrosis of the femoral head

Bioinformatics and Microarray Analysis of miRNAs in Aged Female Mice Model Implied New Molecular Mechanisms for Impaired Fracture Healing

Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

Contact Info

Product

Resources

About