Analysis of allergen profile in patients sensitized to canine allergen and potential Can f 5 cross-reactivity with human PSA

Abstract

Can f 5 allergy and possible cross-reactivity with human semen in which there are significant amounts of prostate-specific antigen (PSA) are particularly interesting aspects of allergy to dog. The objective of the study was to confirm cross-reactivity between human PSA and Can f 5 in a study of canine sensitised women. A total of 100 women (aged 18–73, 41 on average) with a positive history of animal fur allergy or positive skin prick tests to canine allergens were selected. Levels of Immunoglobulin E (IgE) specific to Can f 1, Can f 2, Can f 3, Can f 5 were determined. Patients with increased concentration of sIgE Can f 5 were selected for further inhibition testing using polystyrene microplate ELISA test coated with human PSA. In the studied population, allergy to Can f 5 dominated (52.3% of patients with increased concentration of canine-specific IgE were allergic to this allergenic component). In all analyzed cases, the concentration of IgE Can f 5 decreased after incubation on the ELISA plate coated with human PSA. The minimum decrease in concentration was 10.44%, the maximum was 37.73%, the average decrease was 21.6%. No statistically significant influence of the presence or absence of allergenic sIgE Can f 5 in blood serum on the occurrence of symptoms after intercourse was found. The study confirmed the moderate ability of Can f 5 to cross-react with human PSA sIgE, which may be clinically significant in some women. At the same time, symptoms of an allergy to male semen do not constitute a typical clinical presentation of allergy to Can f 5.

Concentrations of matrix metallopeptidase 9, interleukin 4, and interleukin 8 in follicular fluid, and the results of in vitro fertilization

“…Research on modifying cytological profiles in infertile women would be interesting, but difficult to achieve because of bioethical concerns. 1 , 30 , 31 …”

Experimental Research Models to Assess the Cross-Reactivity between Can f 5 and Human PSA—Two Different Perspectives

“…The suspicion of cross-reactivity between the human PSA and the Can f 5 canine allergen was postulated earlier [ 14 , 15 ]. In our previous publication, we presented the results of an inhibition test in assessing the cross-reactivity between anti-Can f 5 IgE antibodies and human PSA [ 17 ]. The protocols currently presented are an extensive, full version of that experience.…”

“…In our earlier publication [ 17 ], we demonstrated the ability of anti-Can f 5 IgE antibodies to partially bind human PSA. This property is likely due to the similarity between the canine kallikrein molecule and human prostatic kallikrein, which results in the cross-reactivity of antibodies specific to these proteins [ 18 ].…”

“…We proposed two independent research models based on the inhibition assays we described before. The ability of anti-Can f 5 IgE antibodies to block human PSA had been the subject of our research previously [ 17 ].…”

Serum Albumin as a Cross-Reactive Component in Furry Animals May Be Related to the Allergic Symptoms of Patients with Rhinitis

“… 29 Prostatic kallikrein Can f 5 is an androgen-regulated protein expressed in the prostate and hence is present only in male dogs, and there is no strong cross-reactivity between Can f 5 and the extracts from other furry animals. 24 , 30 Lipocalins, as an important sensitizing protein in animals, can often predict the development of IgE cross reactions based on sequence identity, 29 Previous studies have also shown that cross-reactivity occurs only between those lipocalins with a high degree of homology. 11 , 31 However, since only a limited panel of lipocalin components of cat were detected in this study, and no more lipocalin components of other furry animals were commercially available for detection, it may lead to the differences between the results of lipocalin in this study and previous studies.…”