Analysis of allele-specific expression of seven candidate genes involved in lipid metabolism in pig skeletal muscle and fat tissues reveals allelic imbalance of ACACA, LEP, SCD, and TNF

Stachowiak, Monika; Flisikowski, Krzysztof

doi:10.1007/s13353-019-00485-z

Cited by 10 publications

(6 citation statements)

References 20 publications

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“…There have been many controversial studies of the multiple roles involving ACACA in mono- and poly-unsaturated fatty acid content and performance traits [ 54 , 55 ]. Stachowiak et al further reported that ACACA shows a distinct expression pattern in the subcutaneous fat and LD muscle of Landrace pigs [ 56 ]. This indicates that ACACA might be involved in determining the directional deposition of the lipids and this should be investigated further in the future.…”

Section: Discussionmentioning

confidence: 99%

Candidate gene screening for lipid deposition using combined transcriptomic and proteomic data from Nanyang black pigs

Wang

Zhang

et al. 2021

BMC Genomics

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Background Lower selection intensities in indigenous breeds of Chinese pig have resulted in obvious genetic and phenotypic divergence. One such breed, the Nanyang black pig, is renowned for its high lipid deposition and high genetic divergence, making it an ideal model in which to investigate lipid position trait mechanisms in pigs. An understanding of lipid deposition in pigs might improve pig meat traits in future breeding and promote the selection progress of pigs through modern molecular breeding techniques. Here, transcriptome and tandem mass tag-based quantitative proteome (TMT)-based proteome analyses were carried out using longissimus dorsi (LD) tissues from individual Nanyang black pigs that showed high levels of genetic variation. Results A large population of Nanyang black pigs was phenotyped using multi-production trait indexes, and six pigs were selected and divided into relatively high and low lipid deposition groups. The combined transcriptomic and proteomic data identified 15 candidate genes that determine lipid deposition genetic divergence. Among them, FASN, CAT, and SLC25A20 were the main causal candidate genes. The other genes could be divided into lipid deposition-related genes (BDH2, FASN, CAT, DHCR24, ACACA, GK, SQLE, ACSL4, and SCD), PPARA-centered fat metabolism regulatory factors (PPARA, UCP3), transcription or translation regulators (SLC25A20, PDK4, CEBPA), as well as integrin, structural proteins, and signal transduction-related genes (EGFR). Conclusions This multi-omics data set has provided a valuable resource for future analysis of lipid deposition traits, which might improve pig meat traits in future breeding and promote the selection progress in pigs, especially in Nanyang black pigs.

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Section: Discussionmentioning

confidence: 99%

Candidate gene screening for lipid deposition using combined transcriptomic and proteomic data from Nanyang black pigs

Wang

Zhang

et al. 2021

BMC Genomics

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“…Additionally, FABPs are regarded as central modulators of energy homeostasis and lipid metabolism, as they can modulate the uptake of fatty acids and the formation of intracellular lipid droplets [26]. FASN and SCD are key genes that govern lipogenesis and modulate lipid metabolism [27,28]. In this study, GEPIA analyses indicated that FABP6 was positively correlated with FASN and SCD in READ, implying that FABP6 might perform a vital function in lipid metabolism during CRC.…”

Section: Discussionmentioning

confidence: 67%

Kruppel-like factor 5 enhances proliferation, lipid droplet formation and oxaliplatin resistance in colorectal cancer by promoting fatty acid binding protein 6 transcription

Zuo¹,

et al. 2023

Anti-Cancer Drugs

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Oxaliplatin (OXA) is a standard agent for colorectal cancer (CRC) adjuvant chemotherapy. However, acquired and intrinsic OXA resistance is a primary challenge for CRC treatment. This study investigates the function of the Kruppel-like factor 5/fatty acid binding proteins 6 (KLF5/FABP6) axis in CRC proliferation, lipid droplet formation and OXA resistance. OXA-resistant CRC cell lines were constructed, and FABP6 and KLF5 expression was assessed in parental and OXA-resistant CRC cells. Subsequent to gain- and loss-of-function experiments, CRC cell proliferation was assessed by cell counting kit-8 (CCK-8) and clone formation assays, the intracellular lipid synthesis by oil red O staining and the protein expression of lipid metabolism genes by western blot. OXA resistance of CRC cells was assessed by CCK-8 assay. The binding of KLF5 to FABP6 was analyzed by the dual-luciferase reporter and ChIP assays. A tumorigenicity assay in nude mice was adopted to examine the impact of KLF5 on CRC tumor growth and OXA resistance in vivo. FABP6 and KLF5 expression was high in CRC cell lines. Downregulation of FABP6 or KLF5 restrained CRC cell proliferation and lipid droplet formation in vitro. FABP6 and KLF5 expression was elevated in OXA-resistant CRC cells. Downregulation of FABP6 or KLF5 repressed the OXA resistance of OXA-resistant CRC cells. Mechanistically, KLF5 facilitated the transcription of FABP6. FABP6 overexpression counteracted the suppressive effects of KLF5 downregulation on CRC cell growth, lipid droplet formation and OXA resistance. KLF5 downregulation restrained CRC tumor growth and OXA resistance in vivo. In conclusion, KLF5 knockdown reduced FABP6 transcription to protect against proliferation, lipid droplet formation and OXA resistance in CRC.

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“…The association of porcine SCD SNPs with intramuscular fat content was also found in Laiwu pigs and commercial pig breeds [14]. Furthermore, the expression of SCD alleles showed significant allelic imbalance in adipose tissue, and SCD was a potential molecular target for regulating porcine fatness traits [15]. Based on these observations, we hypothesized that SCD plays a key role in porcine adipocyte differentiation.…”

Section: Introductionmentioning

confidence: 74%

Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation

Liu

Wang

Liang

et al. 2020

IJMS

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Fat deposition, which influences pork production, meat quality and growth efficiency, is an economically important trait in pigs. Numerous studies have demonstrated that stearoyl-CoA desaturase (SCD), a key enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, is associated with fatty acid composition in pigs. As SCD was observed to be significantly induced in 3T3-L1 preadipocytes differentiation, we hypothesized that it plays a role in porcine adipocyte differentiation and fat deposition. In this study, we revealed that SCD is highly expressed in adipose tissues from seven-day-old piglets, compared to its expression in tissues from four-month-old adult pigs. Moreover, we found that SCD and lipogenesis-related genes were induced significantly in differentiated porcine adipocytes. Using CRISPR/Cas9 technology, we generated SCD-/- porcine embryonic fibroblasts (PEFs) and found that the loss of SCD led to dramatically decreased transdifferentiation efficiency, as evidenced by the decreased expression of known lipid synthesis-related genes, lower levels of oil red O staining and significantly lower levels of triglyceride content. Our study demonstrates the critical role of SCD expression in porcine adipocyte differentiation and paves the way for identifying it as the promising candidate gene for less fat deposition in pigs.

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Analysis of allele-specific expression of seven candidate genes involved in lipid metabolism in pig skeletal muscle and fat tissues reveals allelic imbalance of ACACA, LEP, SCD, and TNF

Cited by 10 publications

References 20 publications

Candidate gene screening for lipid deposition using combined transcriptomic and proteomic data from Nanyang black pigs

Candidate gene screening for lipid deposition using combined transcriptomic and proteomic data from Nanyang black pigs

Kruppel-like factor 5 enhances proliferation, lipid droplet formation and oxaliplatin resistance in colorectal cancer by promoting fatty acid binding protein 6 transcription

Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation

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