Analysis of active compounds and geographical origin discrimination of Atractylodes macrocephala Koidz. by using high performance liquid chromatography-diode array detection fingerprints combined with chemometrics

Tong, Gao-Yan; Wang, Tong; Chang, Yue-Yue; Chen, Yao; Yang, Jian; Fu, Haiyan; Yang, Xiao‐Long; Li, Xu-Fu; Yu, Ru‐Qin

doi:10.1016/j.chroma.2022.463121

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…The PLS-DA score plot revealed ( Figure 5 ) that A ∼ E enterprises were distributed in five quadrants, indicating significant differences in their chemical composition. Furthermore, variable importance for the projection (VIP) > 1 was utilized as a criterion [ 30 , 31 ] for screening out the main distinction peaks between the distinctive enterprises, as shown in Figure 6 . RD, MPA, DS, SSB, EC, ICA, and JB were the index components of the main differences.…”

Section: Resultsmentioning

confidence: 99%

“…To obtain as many peaks as conceivable while using a straightforward and helpful strategy, three critical parameters controlling extraction yield, including extraction solvent (water; 50/50, 70/30 MeOH/H 2 O (v/v); and EtOH), sample-to-solvent ratio (1-10, 1-25, and 1-50), and ultrasonic extraction time (15,30,45, and 60 min), were studied. e results demonstrated that 1.0 g-25 mL 70% of methanol was the best combination, which yielded more peaks and higher relative peak intensity.…”

Section: Optimization Of the Sample Extraction And Hplc-dad Conditionsmentioning

confidence: 99%

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Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.

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Section: Resultsmentioning

confidence: 99%

Section: Optimization Of the Sample Extraction And Hplc-dad Conditionsmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

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“…In addition, the predicted variable importance projection (VIP) > 1 is used as a standard to measure the influence intensity and explanatory ability of the expression pattern of the compound on the classification discrimination of each group of samples [31,32], thereby assisting in screening the main differences between samples.…”

Section: Discussionmentioning

confidence: 99%

Explorative Study on Volatile Organic Compounds of Cinnamon Based on GC-IMS

Pan,

Qiao,

Liu

et al. 2024

Metabolites

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Cinnamon is one of the most popular spices worldwide, and volatile organic compounds (VOCs) are its main metabolic products. The misuse or mixing of cinnamon on the market is quite serious. This study used gas chromatography-ion migration spectroscopy (GC-IMS) technology to analyze the VOCs of cinnamon samples. The measurement results showed that 66 VOCs were detected in cinnamon, with terpenes being the main component accounting for 45.45%, followed by aldehydes accounting for 21.21%. The content of esters and aldehydes was higher in RG-01, RG-02, and RG-04; the content of alcohols was higher in RG-01; and the content of ketones was higher in RG-02. Principal component analysis, cluster analysis, and partial least squares regression analysis can be performed on the obtained data to clearly distinguish cinnamon. According to the VIP results of PLS-DA, 1-Hexanol, 2-heptanone, ethanol, and other substances are the main volatile substances that distinguish cinnamon. This study combined GC-IMS technology with chemometrics to accurately identify cinnamon samples, providing scientific guidance for the efficient utilization of cinnamon. At the same time, this study is of great significance for improving the relevant quality standards of spices and guiding the safe use of spices.

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“…[ 4 ] and Smilax glabra [ 5 ]. The components of single prescription such as Atractylodes Macocephala Koidz [ 6 ] and Coicis Semen [ 7 ] have been reported, however, the chemicalome and typical metabolome in Zhuling have not been systematically investigated, which will be meaningful to reveal the material basis of its medicinal effect.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effects of Zhuling Jianpi capsule on experimental ulcerative colitis and characterization of its chemical constituents and metabolomics using UHPLC-Q-TOF-MS

Zhang

Liú

et al. 2023

Heliyon

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Analysis of active compounds and geographical origin discrimination of Atractylodes macrocephala Koidz. by using high performance liquid chromatography-diode array detection fingerprints combined with chemometrics

Cited by 4 publications

References 27 publications

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Explorative Study on Volatile Organic Compounds of Cinnamon Based on GC-IMS

Therapeutic effects of Zhuling Jianpi capsule on experimental ulcerative colitis and characterization of its chemical constituents and metabolomics using UHPLC-Q-TOF-MS

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