Analysis of Acidic Oligosaccharides and Glycopeptides by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Papac, Damon I.; Wong, and Anissa; Jones, Andrew J. S.

doi:10.1021/ac960324z

Cited by 274 publications

(220 citation statements)

References 31 publications

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“…Preparation of glycans for MALDI-TOF-MS analysis 36| Analyze permethylated glycans using the DHB matrix (option A) and desialylated native N-glycans isolated from gel-separated glycoproteins and purified glycans using a non-porous graphitized carbon mini-column using the THAP monohydrate matrix (option B) 44 . The THAP monohydrate matrix gives better results than the DHB matrix for sialylated and native glycans, especially when you work on small amounts 45 .…”

Section: |mentioning

confidence: 99%

Analysis of protein glycosylation by mass spectrometry

2007

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We present a detailed protocol for the structural analysis of protein-linked glycans. In this approach, appropriate for glycomics studies, N-linked glycans are released using peptide N-glycosidase F and O-linked glycans are released by reductive alkaline b-elimination. Using strategies based on mass spectrometry (matrix-assisted laser desorption/ionization-time of flight mass spectrometry and nano-electrospray ionization mass spectrometry/mass spectrometry (nano-ESI-MS-MS)), chemical derivatization, sequential exoglycosidase digestions and linkage analysis, the structures of the N-and/or O-glycans are defined. This approach can be used to study the glycosylation of isolated complex glycoproteins or of numerous glycoproteins encountered in a complex biological medium (cells, tissues and physiological fluids).

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Section: |mentioning

confidence: 99%

Analysis of protein glycosylation by mass spectrometry

2007

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“…This can lead to a small amount of polymer nonsolvent in the final mixture, which can affect the signal reproducibility. 18 Therefore, other sample preparation techniques for improved reproducibility and analytic results were developed including vacuum drying, 24 overlayer ͑two-layer or seed layer͒, 25,26 or fast evaporation. 27 All mentioned methods have in common the effort to control the crystallinity of the utilized matrix and therefore the improvement of the analytical results.…”

Section: A General Requirementsmentioning

confidence: 99%

“…The main advantage of the multiple layer technique is the possibility to freely select a solvent/matrix combination that allows good crystallization of the matrix layer and therefore provides improved MALDI-TOFMS results. 19,24,26,27 More- over, a considerable time-saving effect was recognized when comparing the multiple layer spotting technique with the dried droplet technique, since the mixing step is completely removed from the protocol. Figure 2͑a͒ shows optical microscopy pictures of the matrix layer of a 36 member library of MALDI-TOFMS samples that were prepared utilizing the multiple layer approach.…”

Section: B Multiple Layer Spotting For Maldi-tofmsmentioning

confidence: 99%

Integration of MALDI-TOFMS as high-throughput screening tool into the workflow of combinatorial polymer research

Meier

Schubert

2005

Review of Scientific Instruments

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The possibilities of an integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ͑MALDI-TOFMS͒ as an high-throughput screening tool into the workflow of combinatorial materials research are discussed. A multiple layer sample preparation technique for MALDI is described in detail and its possibilities of automation and miniaturization are discussed. Automated MALDI sample preparation could be performed within an automated synthesizer robot as well as with an ink-jet printer. The first approach offers the possibility of online reaction monitoring, whereas the second approach gives the opportunity of applications in ultra-high-throughput environments. Moreover, an example of high-throughput screening of a polymerization reaction by MALDI-TOFMS is discussed.

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“…There are various types of sample preparation methods for MALDI-TOF-MS analysis, such as the drieddroplet [1], fast evaporation [33], vacuum drying [34], sandwich [35], and two-layer [36] methods. Among these techniques, the dried-droplet method is one of the most frequently used for MALDI-TOF-MS analysis in the case of solution (analyte) to solution (matrix) conditions.…”

Section: Optimizing Sample Preparation Methodsmentioning

confidence: 99%

MALDI-TOF-MS analysis of small molecules using modified mesoporous material SBA-15 as assisted matrix

Kim

et al. 2009

J. Am. Soc. Mass Spectrom.

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Mesoporous silica, SBA-15 was successfully functionalized with quinoline moiety, and was applied as a matrix in the MALDI-TOF-MS analysis of small molecules. The modified SBA-15 material propoxy) quinoline, SBA-15-8QSi] was obtained by using calcined SBA-15 and 8-hydroxy quinoline. The structure of the functionalized mesoporous material was systemically characterized by TEM, the N 2 adsorption-desorption isotherm technique and FT-IR spectra. Compared with DHB and SBA-15, SBA-15-8QSi demonstrated several advantages in the analysis of small molecules with MALDI-TOF-MS, such as less background interference ions, high homogeneity, and better reproducibility. Based on these results, the various analytical parameters were optimized. The ideal operating conditions were (1) methanol used as the dissolving solvent; (2) sample first dropping method; (3) a ratio between the analyte and the matrix of 3.5:10. Under these optimization conditions, a low detection limit (8 pmol for L-Arginine-HCl) and high reproducibility (Յ29%) were obtained. This technique was successfully applied to the analysis of various types of small molecules, such as saccharides, amino acids, metabolites, and natural honey. [7], and synthetic polymers [8,9]. However, the application of MALDI-TOF-MS in small molecules analysis has been limited, mainly due to ion interference originating from matrices in the low molecule weight region (Ͻ500 Da) [10,11]. To overcome this problem, several alternative matrices, such as high mass molecules [12,13] There has been a great deal of interest in ordered mesoporous materials due to their regular pore sizes in the mesoporous range, high surface areas, high pore volumes, and easy functionalization since they were first reported in 1992 [23,24]. Recently, there have been significant advances in the preparation and modification of mesoporous materials, which allows their utilization in various applications, such as adsorbents [25], catalyst supports [26], nano-templates [27], and nanomaterials [28] for advanced electronics. In general, the pore surfaces of mesoporous silica materials are covered with a layer of silanol groups (Si-OH), which can act as the binding sites for desired functional groups via covalent grafting on the surfaces. Hu et al. reported on titanium (IV) immobilized mesoporous silica particles which were used as an enriching trap for small molecules (phosphorylated peptides), determined with MALDI-TOF-MS [29]. In addition, mesoporous silica materials have the advantage of optical transparency in the visible region [30].Herein, we successfully functionalized the mesoporous silica, SBA-15 with an 8-hydroxy quinoline group (SBA-15-8QSi hereafter), and applied the functional material as a matrix for MALDI-TOF-MS analysis. The SBA-15-8QSi material exhibited less ion backAddress reprint requests to Dr.

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Analysis of Acidic Oligosaccharides and Glycopeptides by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Cited by 274 publications

References 31 publications

Analysis of protein glycosylation by mass spectrometry

Analysis of protein glycosylation by mass spectrometry

Integration of MALDI-TOFMS as high-throughput screening tool into the workflow of combinatorial polymer research

MALDI-TOF-MS analysis of small molecules using modified mesoporous material SBA-15 as assisted matrix

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