Analysis of a Nuclear-Dna Marker for Species Identification of Adults And
Larvae in the Mytilus Edulis Complex

Wood, Ann R.; Beaumont, Andy R.; Skibinski, D. O. F.; Turner, Gordon

doi:10.1093/mollus/69.1.61

Cited by 36 publications

(25 citation statements)

References 32 publications

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“…The species identity of parents and larvae was confirmed using the Me15 and Me16 primers of Inoue et al (1995), as described in Wood et al (2003). Identity of the female parent of each family was confirmed for a subsample of larvae using a restriction fragment length polymorphism (RFLP) which distinguished F mitotype COIII gene PCR products from the different parents.…”

Section: Methodsmentioning

confidence: 99%

Disruption of doubly uniparental inheritance of mitochondrial DNA in hybrid mussels (Mytilus edulis × M. galloprovincialis)

et al. 2003

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Blue mussels of the genus Mytilus have an unusual mode of mitochondrial DNA inheritance termed doubly uniparental inheritance (DUI). Females are homoplasmic for the F mitotype which is inherited maternally, whereas males are heteroplasmic for this and the paternally inherited M mitotype. In areas where species distributions overlap a varying degree of hybridization occurs; yet genetic differences between allopatric populations are maintained. Observations from natural populations and previous laboratory experiments suggest that DUI may be disrupted by hybridization, giving rise to heteroplasmic females and homoplasmic males. We carried out controlled laboratory crosses between Mytilus edulis and M. galloprovincialis to produce pure species and hybrid larvae of known parentage. DNA markers were used to follow the fate of the F and M mitotypes through larval development. Disruption of the mechanism which determines whether the M mitotype is retained or eliminated occurred in an estimated 38% of M. edulis Â M. galloprovincialis hybrid larvae, a level double that previously observed in adult mussels from a natural M. edulis Â M. galloprovincialis hybrid population. Furthermore, reciprocal hybrid crosses exhibited contrasting types of DUI disruption. The results indicate that disruption of DUI in hybrid mussels may be associated with increased mortality and hence could be a factor in the maintenance of genetic integrity for each species.

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Section: Methodsmentioning

confidence: 99%

Disruption of doubly uniparental inheritance of mitochondrial DNA in hybrid mussels (Mytilus edulis × M. galloprovincialis)

et al. 2003

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“…Identifi cation of M. galloprovincialis and M. edulis (and any hybrids) based on shell shape is usually uncertain because of the extreme plasticity of shape exhibited by the mussels under environmental variation. Since 1995, a DNA-based genetic method that seems truly diagnostic for European populations of these species has become available, Inoue et al ( 1995 ) and Wood et al ( 2003 ) .…”

Section: Distributionmentioning

confidence: 99%

Heavy Metals in Seafood Mussels. Risks for Human Health

Stanković

Jović

Stankovic

et al. 2011

Environmental Chemistry for a Sustainable World

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Life on earth is threatened both by environmental pollution and overpopulation. Natural phenomena contributing to pollution have always existed but anthropogenic activities are increasing contamination of air, soil and water. Waters are especially polluted by metals. The main threats to life from toxic metals are associated with exposure to Pb, Cd, Hg and As, which accumulate in organisms. Overpopulation means more food is required but the amount of arable land is declining

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“…Frischer et al 2000, Paugam et al 2003, surfclams (Bell & Grassle 1998), cockles (André et al 1999) and mussels (e.g. Wood et al 2003, LorenzoAbalde et al 2005 -to a wider range of commonly found species (e.g. Hosoi et al 2004, Larsen et al 2005.…”

Section: Introductionmentioning

confidence: 99%

“…Comtet et al 2000), dot-blot (e.g. Frischer et al 2002), PCR amplification and hybridization (Frischer et al 2000), and multiplex PCR (e.g Wood et al 2003), identification methods developed for a range of species mainly rely on derivations of 2 PCR methods, viz. RFLP (e.g Hosoi et al 2004) and multiplex PCR (e.g.…”

Section: Introductionmentioning

confidence: 99%

In situ hybridization on whole larvae: a novel method for monitoring bivalve larvae

Goff-Vitry¹,

Chipman²,

Comtet³

2007

Mar. Ecol. Prog. Ser.

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Accurate and efficient identification to the species level of early larval stages has long been a problematic step in the study of marine invertebrates, due to the extremely small size of the larvae and their lack of diagnostic morphological characters. It is nonetheless, a prerequisite for any ecological study. As a consequence, a number of molecular approaches, mostly based on the PCR technique, have been developed over the last decade. We developed a method relying on specific rRNA-targeted oligonucleotide probes for in situ molecular hybridization using whole larvae. A colorimetric reaction following the hybridization allows signal detection with a light microscope. A total of 9 probes have been developed, targeting the species that are major components of benthic communities in several European bays. The method can be applied to roughly sorted wild plankton samples, and some steps could be automated. It is relatively inexpensive to implement and does not require costly equipment or expensive staff training. The overall larval morphology is preserved, allowing visual inspection. The quantitative aspect of the approach is another asset. Field-based studies of larval distribution and behaviour are possible applications of the method, as large numbers of samples can be screened to meet the requirements of adequate spatial and temporal coverage. It is also relevant for routine monitoring of target species, such as species of commercial interest, bio-indicators or invasive species.

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Analysis of a Nuclear-Dna Marker for Species Identification of Adults And Larvae in the Mytilus Edulis Complex

Cited by 36 publications

References 32 publications

Disruption of doubly uniparental inheritance of mitochondrial DNA in hybrid mussels (Mytilus edulis × M. galloprovincialis)

Disruption of doubly uniparental inheritance of mitochondrial DNA in hybrid mussels (Mytilus edulis × M. galloprovincialis)

Heavy Metals in Seafood Mussels. Risks for Human Health

In situ hybridization on whole larvae: a novel method for monitoring bivalve larvae

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