Analysis of a Fragment of the Fusion Gene for Newcastle Disease by DNA Sequencing

Abstract

Newcastle disease is caused by Avian Paramyxovirus Type 1 (APMV-1), which is found in the genus Avulavirus and family Paramyxoviridae (Suarez et al., 2020). Mild to severe respiratory, visceral, and central nervous system lesions contribute to the illness's devastating impact on vulnerable flocks.The purpose of this study to analysis of a fragment of the fusion Gene for Newcastle disease virus by DNA sequencing.Three representative Fusion gene for Newcastle disease virus isolates were sequenced by Macrogen, Korea.Alignment of the sequences of the three isolated (AN02, AN03 and AN07) against the registered sequences of Newcastle viruses in the database of NCBI were studied, and the result showed that, 94% identity with avian orthoavulavirus 1 isolate By Ck/IR/MAME96/2017, 99% identity with avian orthoavulavirus 1 isolate INDTNC22020 and 86% identity with avian orthoavulavirus 1 isolate INDTNC22020 respectively.Virulent strain-specific motifs were found in the majority of circulating strains, as shown by partial sequencing of the F gene cleavage site.