Analysis of 5-methyl-deoxycytidine in DNA by micro-HPLC

Randt, C.; Linscheid, Michael

doi:10.1007/bf00481927

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…As an early DNA methylation detection technique, liquid chromatograph (LC) was used to quantitatively detect the global DNA methylation levels. 19 Combined with fluorescence, ultraviolet (UV) and mass spectrometry (MS), the sensitivity of LC for DNA methylation was greatly enhanced. However, due to their inability to obtain detailed methylation information at specific sites, these methods gradually faded out.…”

Section: Dna Methylation Measuring Methodsmentioning

confidence: 99%

Advances in measuring DNA methylation

Sun

Zhu

2021

Blood Science

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DNA methylation is one of the most important components of epigenetics, which plays essential roles in maintaining genome stability and regulating gene expression. In recent years, DNA methylation measuring methods have been continuously optimized. Combined with next generation sequencing technologies, these approaches have enabled the detection of genome-wide cytosine methylation at single-base resolution. In this paper, we review the development of 5-methylcytosine and its oxidized derivatives measuring methods, and recent advancement of single-cell epigenome sequencing technologies, offering more referable information for the selection and optimization of DNA methylation sequencing technologies and related research.

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Section: Dna Methylation Measuring Methodsmentioning

confidence: 99%

Advances in measuring DNA methylation

Sun

Zhu

2021

Blood Science

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“…DNA methylation in bile as a diagnostic biomarker for CCA is feasible, but we are facing a dilemma about how to achieve a simple and rapid detection. Liquid chromatography, which combines fluorescence, UV and mass spectrometry, is an early and sensitive method for the detection of genome-wide DNA methylation, but it cannot obtain the methylation location [ 29 ]. Subsequently, PCR and electrophoresis techniques were used to detect DNA methylation; however, due to heavy workload and low resolution, this method was gradually replaced by microarray technology and sequencing, both of which have the advantages of high throughput and high resolution [ 30 , 31 ].…”

Section: Search For Cca Diagnosis Biomarkers In Human Bile At Gene Levelmentioning

confidence: 99%

Diagnosis Biomarkers of Cholangiocarcinoma in Human Bile: An Evidence-Based Study

Bao

Liu

Chen

et al. 2022

Cancers

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Cholangiocarcinoma (CCA) is a multifactorial malignant tumor of the biliary tract, and the incidence of CCA is increasing in recent years. At present, the diagnosis of CCA mainly depends on imaging and invasive examination, with limited specificity and sensitivity and late detection. The early diagnosis of CCA always faces the dilemma of lacking specific diagnostic biomarkers. Non-invasive methods to assess the degree of CAA have been developed throughout the last decades. Among the many specimens looking for CCA biomarkers, bile has gotten a lot of attention lately. This paper mainly summarizes the recent developments in the current research on the diagnostic biomarkers for CCA in human bile at the levels of the gene, protein, metabolite, extracellular vesicles and volatile organic compounds.

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A GC/MS method for the analysis of 5-methyl-2?-deoxycytidine in DNA

Randt

Linscheid²

1989

Z. Anal. Chem.

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Analysis of 5-methyl-deoxycytidine in DNA by micro-HPLC

Cited by 5 publications

References 17 publications

Advances in measuring DNA methylation

Advances in measuring DNA methylation

Diagnosis Biomarkers of Cholangiocarcinoma in Human Bile: An Evidence-Based Study

A GC/MS method for the analysis of 5-methyl-2?-deoxycytidine in DNA

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