Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana

Cytokinins are plant hormones involved in regulation of diverse developmental and physiological processes in plants whose molecular mechanisms of action are being intensely researched. However, most rapid responses to cytokinin signals at the proteomic and phosphoproteomic levels are unknown. Early cytokinin responses were investigated through proteome-wide expression profiling based on image and mass spectrometric analysis of two-dimensionally separated proteins and phosphoproteins. The effects of 15 min treatments of 7-day-old Arabidopsis thaliana seedlings with four main cytokinins representing hydroxyisopentenyl, isopentenyl, aromatic, and urea-derived type cytokinins were compared to help elucidate their common and specific function(s) in regulating plant development. In proteome and phosphoproteome maps, significant differences were reproducibly observed for 53 and 31 protein spots, respectively. In these spots, 96 proteins were identified by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS), providing a snapshot of early links in cytokinin-regulated signalling circuits and cellular processes, including light signalling and photosynthesis, nitrogen metabolism, the CLAVATA pathway, and protein and gene expression regulation, in accordance with previously described cytokinin functions. Furthermore, they indicate novel links between temperature and cytokinin signalling, and an involvement of calcium ions in cytokinin signalling. Most of the differentially regulated proteins and phosphoproteins are located in chloroplasts, suggesting an as yet uncharacterized direct signalling chain responsible for cytokinin action in chloroplasts. Finally, first insights into the degree of specificity of cytokinin receptors on phosphoproteomic effects were obtained from analyses of cytokinin action in a set of cytokinin receptor double mutants.

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“…Identified proteins were categorized using criteria described by Bevan et al (1998). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling

Černý

Dyčka

Bobáľová

et al. 2010

Journal of Experimental Botany

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“…are given in Supplementary Table S3. Grouping these S-nitrosylated proteins according to their functional category established by Bevan et al (1998), showed that the largest group was associated with photosynthesis (58 %) (Fig. 7c).…”

Section: Effects Of Snp and Nahs On Ptmsmentioning

confidence: 96%

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Ziogas

Tanou

Belghazi³

et al. 2015

Plant Mol Biol

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Emerging evidence suggests that the gaseous molecules hydrogen sulfide (H2S) and nitric oxide (NO) enhances plant acclimation to stress; however, the underlying mechanism remains unclear. In this work, we explored if pretreatment of citrus roots with NaHS (a H2S donor) or sodium nitroprusside (SNP, a NO donor) for 2 days (d) could elicit long-lasting priming effects to subsequent exposure to PEG-associated drought stress for 21 d following a 5 d acclimation period. Detailed physiological study documented that both pretreatments primed plants against drought stress. Analysis of the level of nitrite, NOx, S-nitrosoglutahione reductase, Tyr-nitration and S-nitrosylation along with the expression of genes involved in NO-generation suggested that the nitrosative status of leaves and roots was altered by NaHS and SNP. Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemical treatments, including well known and novel S-nitrosylated targets. Mass spectrometry analysis also enabled the identification of 42 differentially expressed proteins in PEG alone-treated plants. Several PEG-responsive proteins were down-regulated, especially photosynthetic proteins. Finally, the identification of specific proteins that were regulated by NaHS and SNP under PEG conditions provides novel insight into long-term drought priming in plants and in a fruit crop such as citrus in particular.

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“…Assignments were positive when at least four peptides match to ESTs. Proteins were classified into functional categories according to Bevan et al (1998). Non categorized proteins were analyzed using SIMAP (http://webclu.bio.wzw.tum.de/portal/web/simap/seqfinder), ExPASy (http://au.expasy.org/cgi-bin/prosite/ScanView.cgi), NCBI BLASTp (http://blast.ncbi.nlm.nih.gov/Blast.cgi), InterProScan (http://www.ebi.ac.uk/Tools/InterProScan), Scansite (http://scansite.mit.edu/cgi-bin/motifscanseq) and TAIR (http://www.arabidopsis.org/).…”

Section: Validation Of Protein Identification and Functional Annotationmentioning

confidence: 99%

Insights into fruit function from the proteome of the hypanthium

Marondedze

Thomas

2012

Journal of Plant Physiology

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Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana

Cited by 759 publications

References 28 publications

Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling

Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling

Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Insights into fruit function from the proteome of the hypanthium

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