Analysis in vivo of translational mutants of the rIIB cistron of bacteriophage T4

Singer, Britta Swebilius; Gold, Larry; Shinedling, Sidney T.; Colkitt, Michelle; Hunter, Lawrence R.; Pribnow, David; Nelson, Mary Anne

doi:10.1016/0022-2836(81)90479-4

Cited by 63 publications

(30 citation statements)

References 52 publications

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“…In the 6 kb fragment, six putative promoters were defined using the criteria proposed for S. pneumoniae by Sabelnikov et al (1995) ( Table 2). The prokaryotic mRNA ribosome-binding site (RBS) usually contains part or all of the SD sequence UAAGGAGGU (Shine & Dalgarno, 1974) and the spacing between the SD sequence and the initiation codon varies from 5 to 10 nt, as usually observed (Singer et al, 1981;Chen et al, 1994). According to these criteria, six putative SD sequences were proposed ( Fig, 2).…”

Section: Sequence Analysismentioning

confidence: 97%

Organization around the dnaA gene of Streptococcus pneumoniae

et al. 1998

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The dnaA gene region of Streptococcus pneumoniae was cloned and sequenced. A tRNA gene, seven ORFs and three DnaA box clusters were identified. The order of the genes and intergene regions found was tRNAhgorfl-DnaA box cluster 3-htrA-spoOJ-DnaA box cluster 2-dnaA-DnaA box cluster l-dnaN-orfX-orfY. Five ORFs are homologous to known bacterial genes. The tRNAhg gene and orfl, also called orfL, have already been described in pneumococci and have been reported to be preceded by the competence regulation locus comCDE. In Escherichia coli, htrA encodes a serine protease. In Bacillus subtilis, spoOJ plays a role in sporulation and partition. dnaA encodes an initiator replication protein, very well conserved in several bacteria and dnaN encodes the p subunit of DNA polymerase 111 in E. coli. The function of orfX is unknown. The N-terminal part of another reading frame, o m , revealed high homology with a GTP-binding protein. DnaA box clusters were found upstream and downstream from dnaA. The presence of two such clusters suggests that the chromosomal origin of 5. pneumoniae is located within this region. The position of dnaA, and therefore the putative origin of replication, were localized on the physical map of S. pneumoniae.

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Section: Sequence Analysismentioning

confidence: 97%

Organization around the dnaA gene of Streptococcus pneumoniae

et al. 1998

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“…Furthermore, the RNA encoding LIR does not start with the initiator codon AUG, but may start with GUG (indicated as GTG in the DNA sequence presented). While GUG and UUG can be used as an initiator codon in E. coli a small percentage of the time (44) (approximately 8% for GUG and 1% for UUG), generally these codons are less efficient in allowing translation initiation than AUG (42,44). Since the LI' polypeptide begins with a proline, as determined by the amino-terminal sequence, the initiator methionine must be removed either prior to or during formation of the PBS.…”

Section: -Mtsstaarqlgfepfastapteiras---sdvpavihaayrqvfaihvmqserltsamentioning

confidence: 99%

Isolation and characterization of light-regulated phycobilisome linker polypeptide genes and their transcription as a polycistronic mRNA

Lomax¹,

Conley²,

Schilling³

et al. 1987

J Bacteriol

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Several cyanobacteria adjust both the phycobiliprotein and linker protein composition of the phycobilisome, a light-harvesting complex in cyanobacteria and some eucaryotic algae, to maximize absorption of prevalent wavelengths of light. This process is called complementary chromatic adaptation. We sequenced the amino terminus of a linker polypeptide which is associated with phycocyanin and accumulates to high levels during growth of the cyanobacterium Fremyella diplosiphon in red light. A mixed oligonucleotide encoding a region of this amino terminus was synthesized and used to identify a fragment of F. diplosiphon genomic DNA encoding the linker polypeptide. This linker gene was located between two other linker genes and contiguous to the red-light-induced phycocyanin gene set. Sequences of all three linker genes are presented. These genes were transcribed together onto a large polycistronic mRNA which also encoded the red-light-induced phycocyanin subunits. The relationship of this transcript to the biogenesis of the phycobilisome when F. diplosiphon is grown under different conditions of illumination is discussed.

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“…B E (sup °) and B4o (supD; Pribnow et al 1981) were grown at 37°C in M9S medium to an Abs6oo of 0.3, infected at a moi of -7 phage per cell, and assayed as described by Miller (1972). Cultures were sampled and assayed immediately after infection to determine background levels due to basal enzyme activity present in the cell culture and phage stock.…”

Section: -Galactosidase Assaysmentioning

confidence: 99%

“…For comparative purposes, lacZ fusions were also made with exon I sequences from the nrdB (pJZB1) and sunY (pAZH1)genes. To study their in vivo expression, each of these constructs was transferred into the phage genome via recombination between infecting phage DNA and homologous plasmid sequences flanking the fusions (Singer et al 1981;Casna and Shub 1982). Cells containing the fusion plasmids were infected with sah9, a phage containing a deletion of 2400 bp in the region between rIIB and gene 52 (Depew et al 1975).…”

Section: Expression Of the Intron Orfsmentioning

confidence: 99%

Genes within genes: independent expression of phage T4 intron open reading frames and the genes in which they reside.

Gott¹,

Zeeh²,

Bell-Pedersen³

et al. 1988

Genes & Development

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The td, ttrdB, and sunY introns of bacteriophage T4 each contain a long open reading frame (ORF). These ORFs are preceded by functional T4 late promoters and, in the case of the nrdB intron ORF, a functional middle promoter. Expression of phage-encoded intron ORF-IacZ fusions indicates that these T4 genes are highly regulated. The lack of translation of these ORFs from the early pre-mRNAs can be accounted for by the presence of secondary structures that are absent from the late RNAs. Because translation of the intron ORFs could disrupt core structural elements required for pre-mRNA splicing, such regulation may be necessary to allow expression of the genes in which they reside.

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Analysis in vivo of translational mutants of the rIIB cistron of bacteriophage T4

Cited by 63 publications

References 52 publications

Organization around the dnaA gene of Streptococcus pneumoniae

Organization around the dnaA gene of Streptococcus pneumoniae

Isolation and characterization of light-regulated phycobilisome linker polypeptide genes and their transcription as a polycistronic mRNA

Genes within genes: independent expression of phage T4 intron open reading frames and the genes in which they reside.

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