Analysis by two-dimensional electrophoresis of the effect of salt stress on the polypeptide patterns in roots of a salt-tolerant and a salt-sensitive cultivar of wheat

Majoul, Thouraya; Chahed, Karim; Zamiti, Emna; Ouelhazi, Lazhar; Ghrir, Rachid

doi:10.1002/1522-2683(20000701)21:12<2562::aid-elps2562>3.0.co;2-2

Cited by 34 publications

(2 citation statements)

References 15 publications

(9 reference statements)

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“…We demonstrated that there was no newly synthesized protein in the NaCl-treated seedlings compared to the control seedlings in all species. Similar results were reported in the leaves (Ouerghi et al 2000) and roots (Majoul et al 2000) of wheat when the seedlings were grown in 100 mmol/l NaCl for 21 days and in 200 mmol/l NaCl for 4 days, respectively. In contrast to our results, many newly synthesized proteins with low molecular weight were determined in the leaves of wheat (Yıldız 2007), the shoots and roots of barley (Ramagopal 1987) subjected to the NaCl treatments.…”

Section: Resultsmentioning

confidence: 99%

Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors

Yıldız¹,

Terzi²

2008

PLANT SOIL ENVIRON

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The soluble proteins extracted from the first leaf tissues of cultivated tetraploid (Triticum durum Desf., genome AB) and hexaploid (T. aestivum L., genome ABD) wheat species and their diploid wild progenitors [T. monococcum L. (A), Aegilops speltoides Tausch (B), and Aegilops tauschii Cosson (D)] exposed to 100 mmol/l NaCl stress were separated by two-dimensional (2D) gel electrophoresis. There was no newly synthesized protein in the NaCl treatment compared to the control treatment in all species. However, protein profiles showed some differences among species. Most of these proteins had acidic character; their isoelectric point (pI = pH − acidity of proteins) ranged between 5.1-6.9 and low-mol weight (LMW) between 20.3-30.6 kDa. Salt stress caused some proteins to increase or decrease. In the same MW and pI, 11 LMW and 3 intermediate-mol weight (IMW,.4 kDa) proteins increased and/or decreased in amounts were common between at least two species. The remarkable changes in Ae. speltoides were detected as decreases or losses in protein profiles. As a result of salt stress, all the remarkable changes in T. durum were detected as the increases in proteins. However, some proteins increased in T. aestivum, T. monococcum and Ae. tauschii. It is suggested that an increase in the amount of the proteins may lead to an increase in the tolerance mechanisms towards NaCl salinity of wheat species which has A and/or genome(s).

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Section: Resultsmentioning

confidence: 99%

Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors

Yıldız¹,

Terzi²

2008

PLANT SOIL ENVIRON

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“…Shoot length, root length, dry weight and shoot fresh weight, grain yield and expression of genes encoding GS traits were found to be good markers of the response to a salt stress in durum wheat [18,[59][60][61][62]. A number of the genotypes used in our study have already been examined in field or controlled conditions in order to test their physiological and morphological responses in the presence of excess NaCl [37,[63][64][65][66][67][68]. In the present investigation, a panel of genotypes including those previously tested by other research groups, was used in order to study the impact of a salt tress on a much larger portfolio of phenotypic and physiological traits representative of plant growth and development and of primary C and N metabolism.…”

Section: Discussionmentioning

confidence: 99%

Identification of Phenotypic and Physiological Markers of Salt Stress Tolerance in Durum Wheat (Triticum durum Desf.) through Integrated Analyses

et al. 2019

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Salinity is one of the most important stresses that reduces plant growth and productivity in several parts of the world. Nine Tunisian durum wheat genotypes grown under hydroponic conditions were subjected to two levels of salt stress (100 and 170 mM NaCl) for 21 days. An integrative analysis revealing the impact of salinity on key phenotypic and physiological marker traits was then conducted. Principal component analysis grouped these traits into three different clusters corresponding to the absence of salt stress and the two levels of salt stress. This analysis also allowed the identification of genotypes exhibiting various levels of tolerance to NaCl. Among the nine genotypes of Triticum durum Desf., cultivar Om Rabiaa was the most tolerant whereas cultivar Mahmoudi genotype was the most sensitive. Following the multivariate analysis of the examined phenotypic and physiological traits, we found that shoot length, shoot fresh weight, leaf area, the whole-plant stable isotope ratios of nitrogen (δ15N), shoot ammonium and proline contents, and shoot glutamine synthetase activity could be used as markers for the selection of salt-tolerant wheat genotypes.

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Plant Proteomics

Saravanan¹,

Bashir²,

Rose³

2004

Handbook of Plant Biotechnology

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Proteomics, or the study of the protein complement of the genome, promises to help bridge the divide between genomic DNA sequence and biological state and represents one of the most rapidly developing and innovative fields in genome‐scale research. The term is now commonly and broadly used to encompass almost any aspect of protein structure, expression and function. However, as used here it refers to characterising quantitative and qualitative characteristics of global protein expression, with an emphasis on scale, including polypeptide synthesis, degradation, post‐translational modification, the structure of protein complexes and interactions with other proteins and cellular components. The systematic characterisation of complex protein populations can be pursued using many approaches and numerous reviews have recently been published describing developments in diverse areas of proteomics, including protein structure, function and protein–protein, or protein–ligand interactions A number of reviews have also been specifically devoted to plant proteomics. Consequently, the multiplicity of proteomics‐related experimentation and developing technologies are not reiterated here in detail. Instead, this chapter intends to provide an overview of the diversity of proteomics‐based studies that have been reported in plants to date and emerging technologies, which in turn should underscore the tremendous potential utility of proteomics for plant biologists and give an indication of future applications.

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Analysis by two-dimensional electrophoresis of the effect of salt stress on the polypeptide patterns in roots of a salt-tolerant and a salt-sensitive cultivar of wheat

Cited by 34 publications

References 15 publications

Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors

Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors

Identification of Phenotypic and Physiological Markers of Salt Stress Tolerance in Durum Wheat (Triticum durum Desf.) through Integrated Analyses

Plant Proteomics

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