Analysis by scanning force microscopy of enzyme-containing Langmuir-Blodgett films elaborated by a horizontal lifting method

Contribution of Lateral Force and “Tapping Mode” Microscopies to the Study of Mixed Protein Langmuir−Blodgett Films

“…For the characterization of LB films, scanning force microscopy is a unique tool − with its different possibilities; few papers, however, dealing with artificial systems in which proteins are involved have been published. − …”

“…In a series of papers, − we have reported our results obtained by scanning force microscopy for the study of mixed Langmuir−Blodgett films constituted of behenic acid and glucose oxidase transferred on HOPG by horizontal lifting or vertical methods. The images were essentially obtained with the contact mode.…”

Comparison at the microscopic scale of mixed fatty acid-protein Langmuir-Blodgett films resulting from horizontal or vertical transfer

“…Scanning force microscopy, with its different possibilities, is a unique tool for imaging these systems at a microscopic or at a molecular level. Few papers, however, dealing with imaging of mixed layers of proteins and fatty acids have been published [20][21][22][23][24][25][26].…”

“…In a previous paper [26] For the elaboration of the mixed layer of behenic acid and glucose oxidase, an aqueous solution of glucose oxidase was used as subphase, and a behenic acid solution in chloroform was spread on its surface. Aqueous solutions were prepared with water obtained by a Millipore system involving deionization, reverse osmosis and filtration.…”

Selective Solubilization Process Applied to the Study of Enzyme-Containing Langmuir−Blodgett Films

“…A model of the film formed at the air/water interface was also proposed. 6 In previous papers, we reported IR spectroscopy 7 and scanning force microscopy (SFM) 8,9 studies of mixed LB films transferred on HOPG (highly oriented pyrolytic graphite). Recently, we studied the structure of comparable films transferred on mica muscovite, which is a hydrophilic substrate, in order to get stronger interactions between the mixed film and the substrate.…”