Analysis by <i>in Situ</i> Hybridization of Cells Expressing mRNA forTumor‐Necrosis Factor in the Developing Thymus of Mice

Deman, J.; Martin, Marie-Thérèse; Delvenne, Philippe; Humblet, Chantal; Boniver, Jacques; Defresne, Marie-Paule

doi:10.1155/1992/48713

Cited by 26 publications

(11 citation statements)

References 10 publications

(20 reference statements)

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“…Similarly, TNF in vitro supplementation of embryonic lung primordia has a dose‐dependent, stimulating effect on epithelial branching and surfactant‐associated protein expression (Jaskoll et al, 1994a). These studies complement the accumulating evidence that TNF is commonly present during normal embryogenesis (Ohsawa and Natori, 1989; Gendron et al, 1991; Deman et al, 1992; Wride and Sanders, 1993).…”

supporting

confidence: 81%

Embryonic mouse submandibular salivary gland morphogenesis and the TNF/TNF-R1 signal transduction pathway

et al. 2001

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TNF is a pleiotropic cytokine that modulates cell proliferation and apoptosis. The objective of the present study was to investigate the possible function(s) of the TNF/TNF‐R1 signaling pathway in embryonic mouse submandibular salivary gland (SMG) morphogenesis. After characterizing in vivo mRNA and protein expression of various constituents of this pathway, we utilized in vitro experiments to investigate the phenotypic outcomes of enhanced and deficient ligand. The results of these experiments indicate that the TNF/TNF‐R1 signal transduction pathway plays an important role in balancing cell proliferation and apoptosis during SMG duct and presumptive acini formation. Anat Rec 262:318–330, 2001. © 2001 Wiley‐Liss, Inc.

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supporting

confidence: 81%

Embryonic mouse submandibular salivary gland morphogenesis and the TNF/TNF-R1 signal transduction pathway

et al. 2001

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“…Specifically, we provide evidence that TNF is required for optimum survival of peripheral T cells, maximal antigenic responses (both proliferation and Ag-induced apoptosis) as well as tolerance induction. Although we detect the effect of TNF deficiency only in peripheral T cells, we cannot exclude the possibility that a defect due to the absence of TNF at early stages of their development is partly responsible for the observed phenotype, because TNF is known to be expressed in the thymus (46). An important aspect of T cell-mediated immunity is the formation of T cell repertoire and the maintenance of homeostatic equilibrium of mature T cells.…”

Section: Discussionmentioning

confidence: 75%

An Essential Role for TNF in Modulating Thresholds for Survival, Activation, and Tolerance of CD8+ T Cells

Chatzidakis

Fousteri

Tsoukatou

et al. 2007

The Journal of Immunology

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TNF and its receptors p55 and p75 are known to be important in the homeostasis of the peripheral immune system. Previous studies have presented apparently contradictory evidence for an in vivo role of TNF in T cells. In this study, we analyzed TNF-deficient mice crossed with the F5 TCR-transgenic animals. We show that endogenous TNF modulates several aspects of homeostasis of peripheral F5 CD8 T cells. We found that F5/TNF−/−mice had reduced numbers of peripheral F5 T cells, F5/TNF−/− CD8 T cells exhibited reduced survival potential, and furthermore that T cell-derived TNF is required for optimum recovery of naive CD8 T cells in lymphopenic hosts, suggesting its involvement in the survival of peripheral CD8 T cells. Both peptide activation and ensuing Ag-induced apoptosis are quantitatively reduced in TNF−/− CD8 T cells. The latter observations can be related to decreased binding activities of NF-κB and NF-ATp observed in Ag-stimulated F5/TNF−/− T cells. Finally, in a CD8 T cell tolerance model, endogenous TNF was necessary for several parameters of CD8 T cell tolerance induction. Collectively, our results provide evidence that endogenous TNF modulates thresholds in several ligand-driven T cell responses.

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“…In situ hybridizations were done as described 49 with 35 S-labeled RNA probes prepared from a 500 bp PstI-EcoRI of a 5' v-cbl/c-cbl identity region. 23 Fifty microliters of probe mixture of 50% formamide containing NaCl (0.6 M), Tris-HCl (10 mM), EDTA (1 mM), 1% SDS, DTT (10 mM), yeast t-RNA (0.25 mg/ml; Boehringer Mannheim), 16Denhardt's (506Denhardt's=1% Ficoll, 1% polyvinylpyrrolidine, 1% BSA), 10% PEG-6000 (polyethylene glycol-6000) and 1 ml of labeled RNA (150,000 c.p.m./ml) were loaded on each slide and hybridization was performed at 508C overnight in a humid chamber.…”

Section: In Situ Hybridizationsmentioning

confidence: 99%

Nuclear localization of a new c-cbl related protein, CARP 90, during in vivo thymic apoptosis in mice

Denis¹,

Mandard

Humblet

et al. 1999

Cell Death Differ

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This study investigates the involvement of the c-cbl protooncogene in thymocyte apoptosis occuring in vivo after hydrocortisone treatment. In the thymus of untreated mice, a few medullary and cortical thymocytes expressed p120 cbl , mainly in the cytoplasm. In the cortex, their number and distribution resemble that of apoptotic cells evidenced by TUNEL staining. The expression of Cbl is rapidly increased when apoptosis is triggered by hydrocortisone. This Cblspecific immunostaining was detected in the nucleus and is due to a Cbl-related 90 kDa protein (CARP 90). These results show that a c-cbl product could localize in the nucleus and suggest that it could be involved as a regulator of thymic apoptosis.

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Analysis by in Situ Hybridization of Cells Expressing mRNA forTumor‐Necrosis Factor in the Developing Thymus of Mice

Cited by 26 publications

References 10 publications

Embryonic mouse submandibular salivary gland morphogenesis and the TNF/TNF-R1 signal transduction pathway

Embryonic mouse submandibular salivary gland morphogenesis and the TNF/TNF-R1 signal transduction pathway

An Essential Role for TNF in Modulating Thresholds for Survival, Activation, and Tolerance of CD8+ T Cells

Nuclear localization of a new c-cbl related protein, CARP 90, during in vivo thymic apoptosis in mice

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