Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia

Qiburi, Qiburi; Ganbold, Tsogzolmaa; Aoqier, Aoqier; Yang, Dezhi; Su, Zhiyu; Bao, Mingming; He, Meng Xiao; Saren, Gaowa; Temuqile, Temuqile; Baigude, Huricha

doi:10.1016/j.jchromb.2022.123458

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…The up-regulation of these genes at the same time is reported in Qiburi and colleagues' investigation. 25 In the original data from GSE157824, an inverse correlation is reported for the regulation of IGF1 and HMOX1 that may reflect the complex process of plasma effect of cell function.…”

Section: Ppi Network Analysis As a Useful Tool Is Applied To Evaluate...mentioning

confidence: 99%

Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes

et al. 2022

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Introduction: Cold physical plasma is a growing tool in medicine which is applied for the treatment of different cancers. In the present study, the gene profiles of human melanocytes exposed to indirect cold physical plasma versus control individuals are analyzed via protein-protein interaction (PPI) network analysis. Methods: The gene expression profiles were derived from Gene Expression Omnibus (GEO), and the significant differentially expressed genes (DEGs) were decoded via "Expression Atlas". PPI network analysis was applied to find the targeted central genes by indirect cold physical plasma. Results: The main connected component of the constructed network including 74 queried DEGs and 50 added first neighbors was analyzed. Considering degree value, betweenness centrality, closeness centrality, and stress, IGF1 and HMOX1 were introduced as the central nodes. Conclusion: The finding of this study indicates that the down-regulation of IGF1 and the up-regulation of HMOX are the prominent events in response to indirect cold physical plasma treatment at the cellular level. Detection of related biological terms via gene ontology is suggested.

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Section: Ppi Network Analysis As a Useful Tool Is Applied To Evaluate...mentioning

confidence: 99%

Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes

et al. 2022

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“…Eerdun Wurile (EW) consists of 29 herbal medicines and is commonly applied for the treatment of nervous system dysfunction, including memory impairment, blurred facial expression, dizziness, and drowsiness [4]. Currently, the administration of EW in the therapeutic application of neuronal dysfunction is attracting increasing attention and many related studies have been conducted in China.…”

Section: Introductionmentioning

confidence: 99%

Study on the Mechanism of Eerdun Wurile's Effects on Post-operative Cognitive Dysfuntion by the TLR4/NF-κB Pathway

Qiao¹,

et al. 2023

Preprint

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Aims: The object of our work was to observe whether the Mongolian medicine Eerdun Wurile (EW) improve POCD by affecting the TLR4/NF-κB. Methods: Mice (6–8 week-old male C57BL/6J) were selected to establish an animal model of POCD by combining intracerebroventricular injection of lipopolysaccharide and nephrectomy; EW formulation and EW basic formulation were administered intra-gastrically for 7 consecutive days. The cognitive performance was assessed by Morris water maze test. H&E staining was examined to detect alterations in hippocampal tissue. Immunohistochemical staining was performed to evaluate MyD88, NF-κB, TLR4, iNOS, and IBA-1 expressions; Western blotting and RT-qPCR were performed to evaluate MyD88, NF-κB and TLR4. The expressions of IL-6, IL-1β, and TNF-α were evaluated by ELISA. Results: Intracerebroventricular injection of lipopolysaccharide combined with nephrectomy induced cognitive dysfunction in mice, stimulated TLR4/NF-κB and microglia, and promoted the secretion of murine TNF-α, IL-1β and IL-6. EW formulation and EW basic formulation treatment are able to suppress the TLR4/NF-κB pathway activation and microglia, and the serum cytokine secretions related to proinflammation, and restore the cognitive performance. Conclusion: EW formulation and EW basic formulation can improve POCD in mice, and TLR4/NF-κB pathway seems to be one of the important mechanisms in EW's improvement of POCD.

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“…TLR4, a toll-like receptor, is a type I transmembrane type protein widely distributed in brain neurons, microglia and astrocytes [8]. Lipopolysaccharide (LPS) is a specific exogenous ligand for TLR4, which can bind to the receptor TLR4 and activate the MyD88-dependent pathway as a downstream signaling to transduce inflammatory signals and also activate NF-κB (nuclear factor kappa B) [9,10]. NF-κB is a critical nuclear transcription factor in early immune responses and cellular inflammation and promotes the secretion of cytokines for secretion, such as TNF-α (tumor necrosis factor-α), IL-6, IL-1β, and NO [11,12].…”

Section: Introductionmentioning

confidence: 99%

Study on the Mechanism of Eerdun Wurile’s Effects on Post-operative Cognitive Dysfunction by the TLR4/NF-κB Pathway

Qiao,

Li,

et al. 2023

Mol Neurobiol

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The object of our work was to observe whether the Mongolian medicine Eerdun Wurile (EW) improve postoperative cognitive dysfunction (POCD) by affecting the TLR4/NF-κB. Mice (6–8-week-old male C57BL/6 J) were selected to establish an animal model of POCD by combining intracerebroventricular injection of lipopolysaccharide and nephrectomy; EW formulation and EW basic formulation were administered intra-gastrically for 7 consecutive days. The cognitive performance was assessed by Morris water maze test. H&E staining was examined to detect alterations in hippocampal tissue. Immunohistochemical staining was performed to evaluate MyD88, NF-κB, TLR4, iNOS, and IBA-1 expressions; Western blotting and RT-qPCR were performed to evaluate MyD88, NF-κB, and TLR4. The expressions of IL-6, IL-1β, and TNF-α were evaluated by ELISA. Intracerebroventricular injection of lipopolysaccharide combined with nephrectomy induced cognitive dysfunction in mice, stimulated TLR4/NF-κB and microglia, and promoted the secretion of murine TNF-α, IL-1β, and IL-6. EW formulation and EW basic formulation treatment are able to suppress the TLR4/NF-κB pathway activation and microglia, and the serum cytokine secretions related to proinflammation, and restore the cognitive performance. EW formulation and EW basic formulation can improve POCD in mice, and TLR4/NF-κB pathway seems to be one of the important mechanisms in EW’s improvement of POCD.

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Analysis and identification of key anti-inflammatory molecules in Eerdun Wurile and exploration of their mechanism of action in microglia

Cited by 5 publications

References 43 publications

Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes

Identification of Targeted Central Genes (IGF1 and HMOX1) by Indirect Cold Physical Plasma in Human Melanocytes

Study on the Mechanism of Eerdun Wurile's Effects on Post-operative Cognitive Dysfuntion by the TLR4/NF-κB Pathway

Study on the Mechanism of Eerdun Wurile’s Effects on Post-operative Cognitive Dysfunction by the TLR4/NF-κB Pathway

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