Analysis and identification of ADP-ribosylated proteins of Streptomyces coelicolor M145

Penyige, András; Keserű, Judit; Fazakas, Ferenc; Schmelczer, Iván; Szirák, Krisztina; Barabás, György; Bíró, Sándor

doi:10.1007/s12275-009-0032-y

Cited by 11 publications

(9 citation statements)

References 37 publications

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“…5G), was not clearly biased toward MI or MII (Table S1). Some of these genes are well characterized, including glgP , a glycogen phosphorylase up-regulated in MII (up to 16-fold) which participates in glycogen and trehalose metabolism during sequential stages of aerial mycelium development [51] and SCO2008, encoding for a branched chain amino acid binding protein up-regulated in MII (up to 7-fold), which may participate in regulation of morphological differentiation in S. coelicolor [52]. Interestingly, most genes encoding for proteins of “unknown” function were up-regulated in MII (Fig.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures

et al. 2014

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Streptomyces species produce many clinically relevant secondary metabolites and exhibit a complex development that includes hyphal differentiation and sporulation in solid cultures. Industrial fermentations are usually performed in liquid cultures, conditions in which Streptomyces strains generally do not sporulate, and it was traditionally assumed that no differentiation took place. The aim of this work was to compare the transcriptomes of S. coelicolor growing in liquid and solid cultures, deepening the knowledge of Streptomyces differentiation. Microarrays demonstrated that gene expression in liquid and solid cultures were comparable and data indicated that physiological differentiation was similar for both conditions. Eighty-six percent of all transcripts showed similar abundances in liquid and solid cultures, such as those involved in the biosynthesis of actinorhodin (actVA, actII-4) and undecylprodigiosin (redF); activation of secondary metabolism (absR1, ndsA); genes regulating hydrophobic cover formation (aerial mycelium) (bldB, bldC, bldM, bldN, sapA, chpC, chpD, chpE, chpH, ramA, ramC, ramS); and even some genes regulating early stages of sporulation (wblA, whiG, whiH, whiJ). The two most important differences between transcriptomes from liquid and solid cultures were: first, genes related to secondary metabolite biosynthesis (CDA, CPK, coelichelin, desferrioxamine clusters) were highly up-regulated in liquid but not in solid cultures; and second, genes involved in the final stages of hydrophobic cover/spore maturation (chpF, rdlA, whiE, sfr) were up-regulated in solid but not in liquid cultures. New information was also provided for several non-characterized genes differentially expressed in liquid and solid cultures which might be regulating, at least in part, the metabolic and developmental differences observed between liquid and solid cultures.

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Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures

et al. 2014

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“…Here we confirmed auto mono-ADP-ribosylating activity of the SCO5461 protein in vitro , but its specific protein trans targets are yet to be discovered. Several identified ADP-ribosylated proteins in S. coelicolor suggested a connection between protein ADP-ribosylation and the regulation of metabolic requirements of the cells (25, 41). SCO5461 is not conserved across the Streptomyces species (including S. griseus ), suggesting that the major protein ADP-ribosyltransferases in Streptomyces are yet to be uncovered.…”

Section: Discussionmentioning

confidence: 99%

“…Being the best characterized, bacterial DraG (homologue of human ARH1 and ARH3) is a representative of the group of arginine-specific ADP-ribosylhydrolases whose homologues are distributed across all three domains of life. Endogenous ADP-ribosylation has also been reported for some other bacteria, Myxococcus xanthus (18, 19), Mycobacterium smegmatis (20), Bacillus subtilis (21), and Streptomyces representatives (22–25), but little is known about its function in bacteria. Genomic evidence indicates that proteins involved in ADP-ribosylation processing are widespread among bacteria.…”

Section: Introductionmentioning

confidence: 99%

Disruption of Macrodomain Protein SCO6735 Increases Antibiotic Production in Streptomyces coelicolor

Lalić¹,

Marjanović²,

Palazzo

et al. 2016

Journal of Biological Chemistry

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ADP-ribosylation is a post-translational modification that can alter the physical and chemical properties of target proteins and that controls many important cellular processes. Macrodomains are evolutionarily conserved structural domains that bind ADP-ribose derivatives and are found in proteins with diverse cellular functions. Some proteins from the macrodomain family can hydrolyze ADP-ribosylated substrates and therefore reverse this post-translational modification. Bacteria and Streptomyces, in particular, are known to utilize protein ADP-ribosylation, yet very little is known about their enzymes that synthesize and remove this modification. We have determined the crystal structure and characterized, both biochemically and functionally, the macrodomain protein SCO6735 from Streptomyces coelicolor. This protein is a member of an uncharacterized subfamily of macrodomain proteins. Its crystal structure revealed a highly conserved macrodomain fold. We showed that SCO6735 possesses the ability to hydrolyze PARP-dependent protein ADP-ribosylation. Furthermore, we showed that expression of this protein is induced upon DNA damage and that deletion of this protein in S. coelicolor increases antibiotic production. Our results provide the first insights into the molecular basis of its action and impact on Streptomyces metabolism.

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“…DraT homologues are restricted to several nitrogen‐fixing bacteria, while DraG homologues are distributed across all three domains of life . Endogenous ADP‐ribosylation has also been reported for some other bacterial species where this process probably regulates important cellular functions such as sporulation in Bacillus subtilis , development and cell–cell interaction in Myxococcus xanthus , as well as differentiation and secondary metabolism in Streptomyces .…”

Section: Adp‐ribosylation In Bacteriamentioning

confidence: 92%

“…griseus and Streptomyces coelicolor , ADP‐ribosylation patterns change with morphological differentiation and several identified ADP‐ribosylated proteins in Str. coelicolor suggested a connection between protein ADP‐ribosylation and the regulation of metabolic requirements of the cells .…”

Section: Adp‐ribosylation In Bacteriamentioning

confidence: 99%

ADP‐ribosylation: new facets of an ancient modification

2017

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Rapid response to environmental changes is achieved by uni‐ and multicellular organisms through a series of molecular events, often involving modification of macromolecules, including proteins, nucleic acids and lipids. Amongst these, ADP‐ribosylation is of emerging interest because of its ability to modify different macromolecules in the cells, and its association with many key biological processes, such as DNA‐damage repair, DNA replication, transcription, cell division, signal transduction, stress and infection responses, microbial pathogenicity and aging. In this review, we provide an update on novel pathways and mechanisms regulated by ADP‐ribosylation in organisms coming from all kingdoms of life.

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Analysis and identification of ADP-ribosylated proteins of Streptomyces coelicolor M145

Cited by 11 publications

References 37 publications

Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures

Transcriptomic Analysis of Liquid Non-Sporulating Streptomyces coelicolor Cultures Demonstrates the Existence of a Complex Differentiation Comparable to That Occurring in Solid Sporulating Cultures

Disruption of Macrodomain Protein SCO6735 Increases Antibiotic Production in Streptomyces coelicolor

ADP‐ribosylation: new facets of an ancient modification

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