Analysis and Comparison of Zinc Micro Element Concentration Present in Human Seminal Plasma of Asthenospermia and Normospermia Conditions Using Atomic Absorption Spectroscopy for Male Infertility Diagnosis Related to Motility Issues

Abstract

Aim: The major aim of this study is to compare Zinc (Zn) concentration present in human seminal plasma of asthenospermia (motility issues) and normospermia semen samples for identification of clinical relevance. Materials and Methods: Semen samples of normospermia (N=75), asthenospermia (N=75) were collected and semen analysis report has been done by standard world health organization protocol. Zn concentration was evaluated by using atomic absorption spectroscopy (AAS) for both the groups by using standard protocol. Results: Independent sample T-test on fertile and infertile men (asthenospermia and normospermia group) reveals that the zinc concentration is statistically insignificant (P=0.367). Zinc concentration was found to be high in normospermia (mean 2.5 mg/ml) over asthenospermia (mean 0.90 mg/ml) samples. Conclusion: Zn being an essential element for normal functioning for fertilization, its absence or less concentration may lead to infertility. We found high concentration of Zn in normospermia which reflects normal conditions for fertilization and less concentration in asthenospermia reflecting infertile conditions.