Analysis and Characterization of Hematopoietic Progenitor Cells from Fetal Bone Marrow, Adult Bone Marrow, Peripheral Blood, and Cord Blood

Wu, Ai Guo; Michejda, Maria; Mazumder, Amitabha; Meehan, Kenneth R.; Menendez, Frederick A.; Tchabo, Jean-Gilles; Slack, Rebecca; Johnson, Mark P.; Bellanti, Joseph A.

doi:10.1203/00006450-199908000-00006

Cited by 33 publications

(22 citation statements)

References 33 publications

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“…Hematopoietic stem cells seem to display ontogenetic age-dependent differences in growth properties. For example, there is a notably decreased clonogenic potential and proliferative responsiveness in mixed lymphocyte reaction assays observed in adult hematopoietic stem cells as compared with stem cells isolated from fetal bone marrow (19). Because proliferating cells express higher levels of DNA topoisomerase II as compared with quiescent cells (20), a fetus, who has periods of rapid cell turnover, may be more susceptible than an adult to the effect of dietary DNA topoisomerase II inhibitors.…”

mentioning

confidence: 99%

Dietary flavonoids and the MLL gene: A pathway to infant leukemia?

Ross

2000

Proc. Natl. Acad. Sci. U.S.A.

104

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mentioning

confidence: 99%

Dietary flavonoids and the MLL gene: A pathway to infant leukemia?

Ross

2000

Proc. Natl. Acad. Sci. U.S.A.

104

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“…1). These data were obtained from a flow cytometric analysis [26]. The same analysis showed that the FBM cells contained a very low population of cells that expressed the CD3 and CD16 markers, indicating that FBM is least predisposed to induce graft-versus-host disease.…”

Section: Comparative Study Of Stem Cells Derived From Various Sourcesmentioning

confidence: 99%

“…In our earlier work, we demonstrated that FBM has distinctive biological and therapeutic properties, ideal for transplantation. Further, successful engraftment and cellular reconstitution of these cells demonstrated compelling evidence for a higher replicative cell potential of the more imma- ture source of stem cells [24][25][26]. We tested the functional efficacy of FBM from lost pregnancies in these studies, with the goal of determining the best sources of cells for transplantation.…”

Section: Comparative Study Of Stem Cells Derived From Various Sourcesmentioning

confidence: 99%

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Which Stem Cells Should be Used for Transplantation?

Michejda¹

2003

Fetal Diagn Ther

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In view of the ever-increasing demand for human stem cells for transplantation, we initiated in vitro and in vivo studies of human fetal bone marrow stem/progenitor cells derived from lost pregnancies at 16–20 weeks. Utilizing non-human primates as models, we demonstrated that fetal tissue has distinctive biological and therapeutic properties that are optimal for transplantation. Subsequently, we tested and compared the phenotypic and functional characteristics of fetal bone marrow (FBM), adult bone marrow (ABM), and cord blood (CB) and peripheral blood (PB) sources of the most primitive stem/progenitor cells. A striking ontogenic difference in the proportion of CD34+ cells in FBM, ABM, PB and CB was observed (24.6 vs. 2.1 vs. 0.5 vs. 2%). The clonogenic potential, as measured by the CFU-c assay, was also higher in FBM when compared with ABM, PB and CB (202.5 vs. 73.5 vs. 40.8 vs. 65.5 colonies/10⁵cells). Moreover, there was a significant decrease in proliferative responsiveness in the mixed lymphocyte reaction (MLR) assay of FBM and CB as compared to ABM and PB. The cytokinetic profiles of the cells from the four sources were also analyzed. This study revealed that both FBM and ABM had a higher proportion of S-phase (21.7 and 11.5%, respectively), compared to PB and CB cells (1.2 and 2.8%, respectively). FBM and ABM also showed a higher proportion of cells in the G₂-M phase (6.4 and 2.6%, respectively) compared with PB and CB (1.7 and 1.2%, respectively). These data show that FBM has the highest number of proliferating cells. We have also investigated the ontogenic differences in stromal cells derived from FBM, ABM and CB, with a special focus on the expression of selected cytokines, such as CSF, GM-CSF, G-CSF, M-CSF, IL-3, IL-6, IL-10 and IL-11. FBM showed the highest levels of expression of CSF, IL-6 and IL-11 when compared to the other sources. These cytokines may have an important role in engraftment and homing of stem cells. The levels of expression of the other cytokines were similar in all sources of stromal cells, with the exception of G-CSF, which was not detected in CB. Moreover, the number of colonies FBM and ABM cells was higher when inoculated with fetal stromal cells. These results suggested an important regulatory role of cytokines in ontogeny of hematopoiesis. In summary, the foregoing observations indicate that each source of hematopoietic and stromal cells has different intrinsic properties, closely correlated with ontogenetic age, which is a vital determinant for phenotypic characteristics, lineage commitments, immunogenicity as well as proliferative potentials. Our data clearly indicate that FBM is the best source of stem cells for engraftment and therapeutic reconstitution due to its very high proliferative capacity, low immunogenicity and highest number of primitive stem/progenitor cells. It should also be stressed that FBM stem cells retrieved at their optimal stage of hematopoiesis (16–20 weeks) may be the cells of choice for both therapeutic cellular reconstitution...

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“…+ -expressing cells (6,7). It is not known whether CD34 + cells (defined by HUCBC lymphocytes and monocytes that contained over 95% CD34 + -expressing cells) can be used as a prophylactic agent for experimental heatstroke.…”

Section: Introductionmentioning

confidence: 99%

Human Umbilical Cord Blood–Derived CD34+ Cells Can Be Used as a Prophylactic Agent for Experimental Heatstroke

Hwang¹,

Chen²,

Lin³

et al. 2008

J Pharmacol Sci

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Abstract. We attempted to assess the prophylactic effect of human umbilical cord bloodderived CD34+ cells in experimental heatstroke. Anesthetized rats, 1 day before heat stress, were divided into 2 major groups and given CD34 − cells (defined by 1 × 10 6 human cord blood lymphocytes and monocytes that contained <0.2% CD34 + cells) or CD34 + cells (defined by 1 × 10 6 human cord blood lymphocytes and monocytes that contained >95% CD34 + cells). They were exposed to ambient temperature of 43°C for 70 min to induce heatstroke. When the CD34 − cells-treated or untreated rats underwent heat stress, their survival time values were found to be 20 -24 min. Pretreatment with CD34+ cells significantly increased survival time (123 -351 min). As compared with normothermic controls, all CD34− cells-treated heatstroke animals displayed hypotension, hepatic and renal failure, hypercoagulable state, activated inflammation, and cerebral ischemia and injury. However, these heatstroke reactions all were significantly suppressed by CD34 + cells pretreatment. In addition, the levels of interlukin-10 in plasma and glial cell line-derived neurotrophic factors in brain were all significantly increased after CD34 + cell administration during heatstroke. Our data indicate that human umbilical cord-derived CD34 + cells can be used as a prophylactic agent for experimental heatstroke.

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Analysis and Characterization of Hematopoietic Progenitor Cells from Fetal Bone Marrow, Adult Bone Marrow, Peripheral Blood, and Cord Blood

Cited by 33 publications

References 33 publications

Dietary flavonoids and the MLL gene: A pathway to infant leukemia?

Dietary flavonoids and the MLL gene: A pathway to infant leukemia?

Which Stem Cells Should be Used for Transplantation?

Human Umbilical Cord Blood–Derived CD34+ Cells Can Be Used as a Prophylactic Agent for Experimental Heatstroke

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