Analysis and characterization of cultivable heavy metal-resistant bacterial endophytes isolated from Cd-hyperaccumulator Solanum nigrum L. and their potential use for phytoremediation

Luo, Shenglian; Chen, Liang; Chen, Jue-liang; Xiao, Xiao; Xu, Ting; Wan, Yong; Rao, Chan; Liu, Chengbin; Liu, Yu-tang; Lai, Cui; Zeng, Guangming

doi:10.1016/j.chemosphere.2011.07.053

Cited by 217 publications

(76 citation statements)

References 40 publications

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“…The choice of the method employed and its application should take into account the type of contaminant, the type of environment and the amount of the contaminant that is available to the plant; another important aspect is the interaction between the plant and the soil microorganisms, such as bacteria and mycorrhizae. The benefits of these interactions have already been demonstrated (Andrade et al, 2009;Andrade et al, 2010;Khan and Doty, 2011;Luo et al, 2011). The impact of heavy metal exposure on nodule formation and nitrogen fixation in leguminous plants is another important issue that requires more study, with little information available (Matsuda et al, 2002;Obbard and Jones, 2001;Reichman, 2007).…”

Section: Phytoremediationmentioning

confidence: 99%

Use of non-hyperaccumulator plant species for the phytoextraction of heavy metals using chelating agents

Souza

Piotto²,

Nogueirol³

et al. 2013

Sci. agric. (Piracicaba, Braz.)

103

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Soil contamination by heavy metals is a challenge faced by many countries, and engineering technologies to solve this problem are expensive and can cause negative impacts on the environment. One way to minimise the levels of heavy metals in the soil is to use plants that can absorb and accumulate heavy metals into harvestable parts, a process called phytoextraction. Typical plant species used in research involving phytoextraction are heavy metal hyperaccumulators, but plants from this group are not good biomass producers and grow more slowly than most species; thus, they have an important role in helping scientists understand the mechanisms involved in accumulating high amounts of heavy metals without developing symptoms or dying. However, because of their slow growth, it is not practical to use these species for phytoextraction. An alternative approach is to use non-hyperaccumulator plants assisted by chelating agents, which may improve the ability of plants to accumulate more heavy metals than they would naturally. Chelating agents can be synthetic or organic acids, and the advantages and disadvantages of their use in improving the phytoextraction potential of non-hyperaccumulator plants are discussed in this article. We hope to draw attention to ways to improve the phytoextraction potential of non-hyperaccumulator plants that produce a large amount of biomass and to stimulate more research on phytoextraction-inducing substances.

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Section: Phytoremediationmentioning

confidence: 99%

Use of non-hyperaccumulator plant species for the phytoextraction of heavy metals using chelating agents

Souza

Piotto²,

Nogueirol³

et al. 2013

Sci. agric. (Piracicaba, Braz.)

103

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“…Seedlings' length was measured after incubation of the closed Petri dishes for 7 days at 22°C (Belimov et al 2005). The vigor index was determined as follows: % of germination 9 seedling length (Luo et al 2011). …”

Section: Seedling Length Assay In Presence Of Zn and CDmentioning

confidence: 99%

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Pereira

Barbosa

Castro

2014

Int. J. Environ. Sci. Technol.

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In this work, we evaluated the ability of rhizobacteria isolated from a metal-contaminated site to produce plant growth-promoting substances, such as indole-3-acetic acid (IAA), ammonia, hydrogen cyanide, siderophores and extracellular enzymes, to solubilize phosphate and for 1-aminocyclopropane-1-carboxylate (ACC)-deaminase activity, while assessing their capacity to enhance Trifolium repens growth. The rhizobacteria that better performed were used as bioinoculants for plants grown in zinc-and cadmium-spiked soils. Rhizobacteria were inoculated onto white clover seedlings growing in 250 and 500 mg kg -1 of zinc and in 10 and 30 mg kg -1 of cadmium-spiked soils and plant traits determined. The available and exchangeable rhizosphere metal concentrations were analyzed. Twenty rhizobacteria stimulated white clover growth under axenic conditions. Overall, strains Rhodococcus erythropolis EC 34, Achromobacter sp. 1AP2 and Microbacterium sp. 3ZP2 increased clover biomass in the absence and in presence of metals; however, in soils with 250 mg kg -1 of zinc, bacterial inoculation did not enhance plant biomass. Biomass enhancement may be related to the multiple bacterial promoting traits, especially the production of high levels of IAA and siderophores and the activity of ACC-deaminase. To the best of our knowledge, this is the first study reporting the potential of R. erythropolis to increase plant growth in metal-contaminated soils. In general, strains Microbacterium sp. 3ZP2 and Arthrobacter sp. EC 10 enhanced the available and exchangeable metal concentrations in rhizosphere showing their potential to improve phytoremediation efficiency. This work clearly indicates that rhizobacteria are promising for the development of bioinoculants useful for phytoremediation of polluted soils.

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“…Indirect promotion of plant growth by microorganisms can play a key role in extraction and removal of trace elements from the soil because an increase in the biomass causes an increase in the total efficiency of phytoremediation process at the same time (Sessitsch et al, 2013). The microbial stimulation of plants growing in a Cd 2+ -polluted environment, especially by rhizosphere bacteria and/or mycorrhizal fungi, was already confirmed by many experiments, e.g., Baum et al (2006), Azcon et al (2010), Garg and Aggarwal (2011) and Luo et al (2011). Much less attention has been paid to the role of saprophytic fungi in the plant growth promotion process, especially in the presence of heavy metals (Babu et al, 2014).…”

Section: Introductionmentioning

confidence: 56%

Analysis of microbiologically stimulated biomass of Salix viminalis L. in the presence of Cd2+ under in vitro conditions – implications for phytoremediation

Złoch¹,

Tyburski²,

Hrynkiewicz³

2015

Acta Biologica Cracoviensia S. Botanica

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The efficiency of phytoremediation might be highly affected by plant-associated microorganisms, and understanding of the underlying mechanisms is still a great challenge. The primary aim of this study was to evaluate the efficiency parameters for Cd 2+ accumulation in the biomass of willow (Salix viminalis) as well as to define the biochemical response of the host plant when it is inoculated with selected bacterial strains (Massilia sp. and Pseudomonas sp.) or saprophytic fungus (Clitocybe sp.) under controlled in vitro conditions. Inoculation of plants with bacterial strains affected the efficiency of phytoremediation process and was expressed as the quantity of accumulated Cd (Q), the bioaccumulation factor (BCF) and the translocation index (Ti); however, the effect was strain and plant organ specific. The level of hydrogen peroxide (H 2 O 2 ), which is both an indicator of plant response to biological and/or abiotic environmental stress and a molecule involved in plant-microbial interactions, decreased under the influence of Cd 2+ in uninoculated plants (plant growth was inhibited by Cd 2+ ) and increased in the inoculated variants of plants growing in the presence of Cd 2+ (microbiologically stimulated biomass). The saprophytic fungus Clitocybe sp. generally stimulated biomass and increased the level of H 2 O 2 synthesis in all the investigated plant organs and variants of the experiment. We suggest that determination of phytoremediation efficiency, and biochemical response (H 2 O 2 ) of the host plant under in vitro conditions can help in predicting the final effect of plant-microbial systems in further field trials. K Ke ey y w wo or rd ds s: : willow, bioremediation, cadmium, hydrogen peroxide (H 2 O 2 )

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Analysis and characterization of cultivable heavy metal-resistant bacterial endophytes isolated from Cd-hyperaccumulator Solanum nigrum L. and their potential use for phytoremediation

Cited by 217 publications

References 40 publications

Use of non-hyperaccumulator plant species for the phytoextraction of heavy metals using chelating agents

Use of non-hyperaccumulator plant species for the phytoextraction of heavy metals using chelating agents

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Analysis of microbiologically stimulated biomass of Salix viminalis L. in the presence of Cd2+ under in vitro conditions – implications for phytoremediation

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