Analysis and application evaluation of the flavour-precursor and volatile-aroma-component differences between waste tobacco stems

Fan, Xing; Zi, Wenhua; Ao, Jincheng; Li, Boyu; Qiao, Junfei; Wang, Yong; Nong, Yonghong

doi:10.1016/j.heliyon.2022.e10658

Cited by 10 publications

(4 citation statements)

References 25 publications

(38 reference statements)

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“…The production of NAECs and the degradation of nicotine during fermentation by the Klebsiella variicola H8 strain and native microbial strains was determined using GC-MS (Agilent, Santa Clara, CA, USA) [ 26 , 27 ] and was quantified in all five samples, CK (blank), H8 (8 h), H16 (16 h), H24 (24 h), and H36 (36 h), each in triplicate. For the extraction process, 20.5 mL of the sample was added to a 1000 mL round-bottom flask containing ddH 2 O (500 mL), 2,6-dichlorotoluene (100 μL), and dichloromethane (100 mL).…”

Section: Methodsmentioning

confidence: 99%

Unravel the Supremacy of Klebsiella variicola over Native Microbial Strains for Aroma-Enhancing Compound Production in Reconstituted Tobacco Concentrate through Metagenomic Analysis

Huang,

Zhu,

Wang

et al. 2024

Metabolites

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Sensory attributes strongly influence consumers’ preferences for products. The inoculation of the Klebsiella variicola H8 strain in a reconstituted tobacco leaf concentrate (RTLC) solution increased neutral aroma-enhancing compound (NAEC) production by 45%, decreased the nicotine level by 25%, decreased the water-soluble total sugar content by ~36%, and improved the sensory quality by 5.71%. The production of NAECs such as dihydrokiwi lactone (DHKL: 192.86%), 1,2,3,4-tetrahydro-1,1,6-trimethylnaphthalene (THTMN: 177.77%), 2,4-di-tert-butylphenol (DTBP: 25%), 4-oxoisofolkone (OIFK: 116.66%,) 1,9-heptadecadiene-4,6-diyn-3-ol (HDD: 116.67%), β-damastrone (BDS: 116.67), and megastigmatrienone A (MSTA: 116.67%) was increased. A metagenomics analysis of the microbial community in the fermented RTLC (FRTLC) was performed to elucidate the mechanism by which NAECs were produced. As a result, 24 groups of functional genes were identified, and among them, five families of carbohydrate-active enzymes, (i) glycoside hydrolase (GH), (ii) glycosyltransferase (GT), (iii) polysaccharide lyase (PL), (iv) carbohydrate esterase (CE), and (v) auxiliary active enzyme (AA), were found to be positively correlated with the production of NAECs. However, among the GHs, the GHs annotated from the H8 strain chromosome displayed the highest relative abundance and a positive correlation with the production of NAECs. Specifically, the GH13-14, GH13-20, GH13-38, GH13-25, GH13-10, GH42, and GH28 genes of the H8 strain were relatively more abundant and were key contributors to the production of NAECs. The correlation analyses revealed that the H8 strain plays a leading role among all the microorganisms in FRTLC in the production of NAECs. Our findings support the application of Klebsiella variicola in NAEC production and a reduction in nicotine content in tobacco products.

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Section: Methodsmentioning

confidence: 99%

Unravel the Supremacy of Klebsiella variicola over Native Microbial Strains for Aroma-Enhancing Compound Production in Reconstituted Tobacco Concentrate through Metagenomic Analysis

Huang,

Zhu,

Wang

et al. 2024

Metabolites

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“…The evaluation of the quality of flue-cured tobacco (FCT) is mainly based on the sensory evaluation of quality and flavour style. The evaluation of sensory quality includes gas diffusivity, agglomeration, aroma quality, aroma quantity, sweetness, softness, dryness, irritation, miscellaneous gas, and aftertaste [ [2] , [3] , [4] ]. The factors influencing tobacco quality are complex and varied; however, the intrinsic chemical components of tobacco leaves are the main factors determining the quality of FCT [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Application of PLSR in correlating sensory and chemical properties of middle flue-cured tobacco leaves with honey-sweet and burnt flavour

Li,

Ma,

Dai

et al. 2024

Heliyon

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“…Among them, tobacco stems have a rich fiber content (up to 23%), which are higher than that of the leaf lamina. However, as a by-product of the tobacco production, more than 60% of tobacco stems are disposed of as garbage worldwide after the tobacco leaves are harvested (Chen et al 2017;Yan et al 2018;Zhang et al 2019a), which leads to a considerable waste of natural plant resources and causes serious environmental pollution (Fan et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Preparation of spherical nanocellulose from waste tobacco stem

Luo,

Xu,

Jiang

et al. 2024

BioRes

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Tobacco stems constitute a large amount of waste biomass generated during tobacco production, and their recycling is of great significance to the environment and the conservation of resources. In this study, an efficient, inexpensive, and less toxic strategy is reported for recycling waste tobacco stem, wherein the spherical tobacco stem nanocellulose (STsN) with a size of 10 to 100 nm was prepared from waste stems using a NaOH/urea/thiourea aqueous system. The morphology of STsN was characterized using scanning electron microscopy. The crystal structure of STsN was determined using X-ray diffractometry. The nanocellulose exhibited the crystal structure of cellulose II. Fourier-transform infrared spectra of the STsN indicated that STsN retained the typical chemical structure of cellulose. The thermal properties of STsN were investigated by thermogravimetry. It is concluded that the STsN had better thermal stability than cellulose. The product has potential for practical application with high thermal stability requirements, such as transistors and batteries.

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Analysis and application evaluation of the flavour-precursor and volatile-aroma-component differences between waste tobacco stems

Cited by 10 publications

References 25 publications

Unravel the Supremacy of Klebsiella variicola over Native Microbial Strains for Aroma-Enhancing Compound Production in Reconstituted Tobacco Concentrate through Metagenomic Analysis

Unravel the Supremacy of Klebsiella variicola over Native Microbial Strains for Aroma-Enhancing Compound Production in Reconstituted Tobacco Concentrate through Metagenomic Analysis

Application of PLSR in correlating sensory and chemical properties of middle flue-cured tobacco leaves with honey-sweet and burnt flavour

Preparation of spherical nanocellulose from waste tobacco stem

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