Analysis and Antibacterial Activity of <i>Nigella sativa</i> Essential Oil Formulated in Microemulsion System

Finding novel antibiotic substances from medicinal plants — Antimicrobial properties of Nigella sativa directed against multidrug resistant bacteria

“…In another set of experiments, the antibacterial activities of black cumin essential oil (EO) were tested against three Gram-positive and three Gram-negative bacterial strains, namely, S. aureus, Bacillus cereus, Listeria monocytogenes , and S. typhimurium, Escherichia coli, Pseudomonas aeruginosa , respectively [11]. The synthetic antibiotic ceftriaxone and a naturally derived antimicrobial named eugenol served as control compounds.…”

“…The EO microemulsion displayed its highest antibacterial activity in a minimum concentration of 100 μg EO per well against S. aureus and B. cereus with inhibition zones of 15.7 mm and 14.0 mm, respectively. As compared to eugenol microemulsion, black cumin microemulsion exerted higher antibiotic activity against S. aureus, B. cereus , and S. typhimurium in all concentrations [11]. …”

“…At 500 μg/well, N. sativa EO was found effective against E. coli as indicated by a mean inhibition zone of 30.3 mm, that was comparable to that exerted by ceftriaxone (31.3 mm) at the same concentration. L. monocytogenes and P. aeruginosa , however, were tested resistant to EO at all applied concentrations [11]. …”

“…N. sativa seeds contain oil, protein, carbohydrate, fiber, and saponin. The fixed oil is composed of arachidonic acid, linoleic acid, oleic acid, almitoleic acid, palmitic acid, stearic acid, myristic acid, steroles, and eicosadenoic acid [9], whereas the essential oil of N. sativa entails nigellone, thymoquinone, thymohydroquinone, thymol, carvacrol, α- and β-pinene, d-limonene, d-citronellol, and p-cymeme [10, 11]. …”

In vitro Antibacterial activity of Nigella Sativa extract against clinical Isolates of Methicillin Resistant Staphylococcus Aureus

“…N. sativa seeds contain oil, protein, carbohydrate, fiber, and saponin. The fixed oil is composed of arachidonic acid, linoleic acid, oleic acid, almitoleic acid, palmitic acid, stearic acid, myristic acid, steroles, and eicosadenoic acid (14) , whereas the essential oil of N. sativa entails nigellone, thymoquinone, thymohydroquinone, thymol, carvacrol, α-and β-pinene, d-limonene, d-citronellol, and p-cymeme (15)(16) .…”