Analyses of the function of DnaJ family proteins reveal an underlying regulatory mechanism of heat tolerance in honeybee

Li, Guilin; Zhao, Hang; Guo, Hongbin; Wang, Ying; Cui, Xuepei; Li, Han; Xu, Baohua; Guo, Xingqi

doi:10.1016/j.scitotenv.2020.137036

Cited by 22 publications

(21 citation statements)

References 70 publications

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“…MED16 is recruited as the HSP gene promoter in response to heat stress (S. Kim & Gross, 2013 ). Previous studies have established that knockdown of DNAJC8 decreases antioxidant defenses and increases oxidative damage in honeybees, while DNAJC8 has been shown to function significantly under heat stress in honeybees (G. Li G et al, 2020 ). Moreover, HSPA4 promotes cellular protection against thermal damage and prevents protein denaturation ( Niu et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Analyses Reveal Genomic Characteristics and Selection Signatures of Lincang Humped Cattle at the China–Myanmar Border

Sun

et al. 2022

Front. Genet.

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The location on the Yunnan border with Myanmar and its unique cultural landscape has shaped Lincang humped cattle over time. In the current study, we investigated the genetic characteristics of 22 Lincang humped cattle using whole-genome resequencing data. We found that Lincang humped cattle derived from both Indian indicine and Chinese indicine cattle depicted higher levels of genomic diversity. Based on genome-wide scans, candidate genomic regions were identified that were potentially involved in local thermal and humid environmental adaptions, including genes associated with the body size (TCF12, SENP2, KIF1C, and PFN1), immunity (LIPH, IRAK3, GZMM, and ELANE), and heat tolerance (MED16, DNAJC8, HSPA4, FILIP1L, HELB, BCL2L1, and TPX2). Missense mutations were detected in candidate genes IRAK3, HSPA4, and HELB. Interestingly, eight missense mutations observed in the HELB gene were specific to the indicine cattle pedigree. These mutations may reveal differences between indicine and taurine cattle adapted to variable climatic conditions. Our research provides new insights into the genetic characteristics of Lincang humped cattle representing Lincang and Pu’er areas as an important channel for the migration of Indian indicine from domestication centers toward southwestern China.

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Section: Discussionmentioning

confidence: 99%

Whole-Genome Analyses Reveal Genomic Characteristics and Selection Signatures of Lincang Humped Cattle at the China–Myanmar Border

Sun

et al. 2022

Front. Genet.

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“… 34 HSP family functions are related to temperature changes. 35 However, under NaCl stress, the root-cap length of OSHSP40 transgenic seedlings was significantly shorter than that of wild-type seedlings. The results showed that HSP40 was related to salt stress.…”

Section: Discussionmentioning

confidence: 92%

Functional verification and screening of protein interacting with the slPHB3

Chang

et al. 2022

Plant Signaling & Behavior

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slPHB3 was cloned from Salix linearistipularis , the amino acid sequence blast and phylogenetic tree analysis showed that slPHB3 has the most similarity with PHB3 from Populus trichocarpa using DNAMAN software and MEGA7 software. RT-qPCR results confirmed that the expression of slPHB3 was induced obviously under stress treatments. The growth of recombinant yeast cells was better than that of the control group under the stress treatment, indicating that slPHB3 may be involved in the stress response of yeast cells. The transgenic tobacco was treated with different concentrations of NaCl, NaHCO 3 and H 2 O 2 , fresh weigh of overexpression tobacco were heavier than wild-types. The results showed that transgenic tobacco was more tolerant to salt and oxidation than wild-type tobacco. Expression of important genes including NHX1 and SOS1 in salt stress response pathways are steadily higher in overexpression tobacco than that in wild-types. We identified 17 proteins interacting with slPHB3 by yeast two-hybrid technique, most of these proteins were relation to the stresses. The salt tolerance of slPHB3 expressing yeast and slPHB3 overexpressing plants were better than that of the control. Ten stress-related proteins may interact with slPHB3, which preliminarily indicated that slPHB3 had a certain response relationship with salt stress. The study of slPHB3 under abiotic stress can improve our understanding of PHB3 gene function.

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“…The most important function of DnaJ is to promote recovery of misfolded and damaged proteins in cells subjected to environmental stresses and insecticides ( Chen et al 2018 ). In the honeybee Apis cerana Fabricius (Hymenoptera, Apidae), two DnaJC subfamily genes ( DnaJC3 and DnaJC8 ) are required for the tolerance to stress conditions such as exposure to heat, abamectin, and lambda-cyhalothrin ( Li et al 2018 , Zhang et al 2019 , Li et al 2020 ). Previous studies demonstrated that trans-anethole can cause abnormally high-level reactive oxygen species (ROS), leading to oxidative damage in insect cells ( Shahriari et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole

Ding

et al. 2021

Journal of Insect Science

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Plant essential oils, with high bioactivity and biodegradability, provide promising alternatives to synthetic pesticides for pest control. Trans-anethole is the major component of essential oil from star anise, Illicium verum Hook. The compound has a strong contact toxicity against the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), which is a major insect pest of many vegetables and crops. However, little information is known about how M. persicae responds to trans-anethole at the molecular level. We conducted a comparative transcriptome analysis of M. persicae in response to a LD50 dose of trans-anethole. A total of 559 differentially expressed genes were detected in the treated individuals, with 318 genes up-regulated, and 241 genes down-regulated. Gene ontology (GO) analysis revealed that these genes were classified into different biological processes and pathways. We also found that genes encoding ATP-binding cassette (ABC) transporters, DnaJ, and cuticle proteins were dramatically up-regulated in response to trans-anethole. To study the function of these genes, we performed RNA interference (RNAi) analysis. Knockdown of an ABC transporter gene (ABCG4) and a DnaJ gene (DnaJC1) resulted in a significantly increased mortality rate in M. persicae following trans-anethole exposure, indicating the involvement of these two genes in the toxicity response to trans-anethole. The findings provide new insights into the mechanisms of M. persicae in coping with plant essential oils.

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Analyses of the function of DnaJ family proteins reveal an underlying regulatory mechanism of heat tolerance in honeybee

Cited by 22 publications

References 70 publications

Whole-Genome Analyses Reveal Genomic Characteristics and Selection Signatures of Lincang Humped Cattle at the China–Myanmar Border

Whole-Genome Analyses Reveal Genomic Characteristics and Selection Signatures of Lincang Humped Cattle at the China–Myanmar Border

Functional verification and screening of protein interacting with the slPHB3

Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole

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