Analyses of long non-coding RNA and mRNA profiling in the spleen of diarrheic piglets caused by Clostridium perfringens type C

Abstract

BackgroundClostridium perfringens (C. perfringens) type C is the most common bacteria causing piglet diarrheal disease and it greatly affects the economy of the global pig industry. The spleen is an important immune organ in mammals; it plays an irreplaceable role in resisting and eradicating pathogenic microorganisms. Based on different immune capacity in piglets, individuals display the resistance and susceptibility to diarrhea caused by C. perfringens type C. Recently, long non-coding RNA (lncRNA) and mRNA have been found to be involved in host immune and inflammatory responses to pathogenic infections. However, little is known about spleen transcriptome information in piglet diarrhea caused by C. perfringens type C.MethodsHence, we infected 7-day-old piglets with C. perfringens type C to lead to diarrhea. Then, we investigated lncRNA and mRNA expression profiles in spleens of piglets, including control (SC), susceptible (SS), and resistant (SR) groups.ResultsAs a result, 2,056 novel lncRNAs and 2,417 differentially expressed genes were found. These lncRNAs shared the same characteristics of fewer exons and shorter length. Bioinformatics analysis identified that two lncRNAs (ALDBSSCT0000006918 and ALDBSSCT0000007366) may be involved in five immune/inflammation-related pathways (such as Toll-like receptor signaling pathway, MAPK signaling pathway, and Jak-STAT signaling pathway), which were associated with resistance and susceptibility to C. perfringens type C infection. This study contributes to the understanding of potential mechanisms involved in the immune response of piglets infected with C. perfringens type C.

Identification and Characterization of MAPK Signaling Pathway Genes and Associated lncRNAs in the Ileum of Piglets Infected by Clostridium perfringens Type C

“…GADD45G may participate in the regulation of cell apoptosis by activating the MAPK signaling pathway [50]. Yan et al found that GADD45G was differentially expressed in the spleen tissue of piglets infected with C. perfringens [51]. In our study, GADD45G had the highest expression in the IS group, indicating this gene is closely related to intestinal damage and enterocyte death in the ileum of those piglets sensitive to C. perfringens type C.…”

Integrated analysis of lncRNA, miRNA and mRNA reveals novel insights into the fertility regulation of large white sows

“…On the other hand, miRNAs are an abundant class of short (18–24 nucleotides) and highly conserved sequences of endogenous RNAs, which have been extensively studied in many species [ 14 ]. Previous studies have prospectively confirmed that ncRNAs (miRNAs and lncRNAs) were considered as crucial players in both humans and animals, and affect various biological functions [ 12 , 15 – 17 ]. Moreover, there is substantial evidence supporting that lncRNAs, acting as competing endogenous RNAs (ceRNAs), contribute to the regulation of cardiac fibrosis [ 18 ], muscle differentiation [ 19 ] and tumorigenesis [ 20 , 21 ].…”

Exploration of Long Non-coding RNAs and Circular RNAs in Porcine Milk Exosomes

“…Interestingly, the differentially expressed lncRNA (ALDBSSCG0000001631) involved in ileum immune response in CPID was found in porcine milk exosome. They also reported the potential lncRNA ( Yan et al, 2018a ) and circRNA ( Yan et al, 2018b ) functions in the spleen of diarrheic piglets caused by CPID. Liu et al (2018) and Chen et al (2019) revealed the potential role of lncRNA or circRNA in muscle and fat development in piglets.…”