Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application

Harada, Daisuke; Takada, Chie; Tsukumo, Yukihito; Takaba, Katsumi; Manabe, Haruhiko

doi:10.1016/j.jdermsci.2004.11.007

Cited by 30 publications

(29 citation statements)

References 30 publications

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“…As these immunological changes are also observed in patients with atopic dermatitis, this chronic dermatitis model may be a suitable model of human atopic dermatitis (6,7). However, although atopic dermatitis-like skin inflammations have been reported in this model, there are few reports regarding the itch-associated response.…”

Section: Introductionmentioning

confidence: 85%

“…It has been reported that repeated applications of a hapten such as oxazolone to mouse skin induced chronic dermatitis, which is associated with increased haptenspecific IgE in serum and increased T-helper 2 cytokines such as interleukin-4 at the chronic dermatitis site (6,7). As these immunological changes are also observed in patients with atopic dermatitis, this chronic dermatitis model may be a suitable model of human atopic dermatitis (6,7).…”

Section: Introductionmentioning

confidence: 90%

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Pharmacological Characterization of Itch-Associated Response Induced by Repeated Application of Oxazolone in Mice

2010

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Abstract. We investigated pharmacological characteristics of the itch-associated response to chronic dermatitis induced by 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone) repeated application in mice. Application of an oxazolone challenge to mice with oxazolone-induced chronic dermatitis evoked severe and transient scratching behavior for up to 1h. Thereafter, mild and continuous scratching behavior was observed for at least 8 h. Both severe and continuous scratching behaviors were suppressed by the opioid-receptor antagonist naltrexone, but not by the H 1 histamine-receptor antagonist fexofenadine, 5-hydroxytryptamine-2 (5-HT 2 )-receptor antagonist methysergide, NK 1 -receptor antagonist LY303870, cyclooxygenase inhibitor indomethacin, or the platelet-activating factor-receptor antagonist YM264. The severe scratching behavior was suppressed by the 5-lipoxygenase inhibitor zileuton and leukotriene B 4 -receptor antagonist ONO-4057, but not by the cysteinyl leukotriene-receptor antagonist montelukast. The continuous scratching behavior was suppressed by pretreatment with the non-selective muscarinic acetylcholine-receptor antagonist atropine and M 3 muscarinic acetylcholine-receptor antagonist darifenacin. These results suggest that leukotriene B 4 receptor and M 3 muscarinic acetylcholine receptor are involved in the itch-associated response induced by repeated application of oxazolone in mice.

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Section: Introductionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 90%

Pharmacological Characterization of Itch-Associated Response Induced by Repeated Application of Oxazolone in Mice

2010

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“…At present, there are several animal models of dermatitis with atopic dermatitis-like skin lesions. For example, repeated epicutaneous exposure to a chemical compound [3,4,5,6] or protein antigen [7] resulted in chronic contact hypersensitivity. NC/Nga mice developing atopic dermatitis-like inflammation with spontaneous hyperproduction of IgE under conventional conditions [8] have also been used as a model of atopic dermatitis [9, 10].…”

Section: Introductionmentioning

confidence: 99%

Modification of the Picryl Chloride-Induced Allergic Dermatitis Model in Mouse Ear Lobes by 12-<i>O</i>-Tetradecanoylphorbol 13-Acetate, and Analysis of the Role of Histamine in the Modified Model

Hirasawa

Ohsawa

Katoh

et al. 2008

Int Arch Allergy Immunol

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Background: In atopic dermatitis, inflammation induced by antigen-nonspecific stimuli further enhances the allergic inflammation. However, there is no experimental model in which allergic dermatitis is evoked where the inflammation has been induced by antigen-nonspecific stimuli. Here, we established a novel dermatitis model in mice and analyzed the role of histamine. Methods: After sensitization with picryl chloride (PiCl) by painting on ear lobes of cyclophosphamide-treated mice, 12-O-tetradecanoylphorbol 13-acetate (TPA) was painted twice at the same site, and then allergic inflammation was induced by painting PiCl. Histamine antagonists and cyclosporine A (CsA) were administered intravenously. Results: The application of TPA shifted the PiCl-induced allergic inflammation from a delayed-type response to a biphasic response, increased the infiltration of eosinophils and mast cells at the inflammatory site, shifted the cytokine milieu from Th1 to Th2 and induced the expression of thymic stromal lymphopoietin in the ear lobes. The PiCl-induced increase in the thickness of the ear lobe in the immediate phase was suppressed by the H1 antagonist pyrilamine. In contrast, the increase in the swelling in the late phase and the infiltration of eosinophils were suppressed by the H3/H4 antagonist thioperamide. The inhibitory effect of the combined treatment with pyrilamine and thioperamide on the TPA-modified contact dermatitis was as potent as that of CsA. Conclusion: Induction of the antigen-nonspecific inflammation by TPA enhanced the PiCl-induced allergic inflammation. Histamine plays significant roles in the early-phase swelling via H1 receptors, and the late-phase swelling via H3/H4 receptors in this TPA-modified allergic dermatitis model.

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“…Moreover, it was reported that repeated application of TNCB resulted in a site-restricted shift in the time course of antigen-specific hypersensitivity responses from a typical delayed-type to an early-type response (Harada et al, 2005;Kitagaki et al, 1995;Kitagaki et al, 1997). In delayed-type allergy, the levels of cytokine mRNA increased up to 3 h and then decreased after hapten application.…”

Section: Fig 2 Effect Of Feeding Adlay Leaves On Serum Ige Levels Imentioning

confidence: 99%

Effect of Administration of Adlay Leaves on 2,4,6-trinitro-1-chlorobenzene-induced Chronic Dermatitis in Mice

Hayashi

Takegawa²,

Tsuzuki³

et al. 2009

FSTR

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Diets containing either dehulled adlay seeds or young adlay leaves were administered to mice. The hapten 2,4,6-trinitro-1-chlorobenzene (TNCB) was applied to the right ear of mice daily for 28 days to generate a model of chronic dermatitis. The thickness of the right ear was measured to evaluate the pathology of allergy. The symptoms of mice fed diet containing adlay leaves were reduced. The serum IgE levels of mice belonging to both adlay groups were significantly lower than the control group. The IFN-γ level of mice belonging to the adlay leaf group was significantly higher in these groups and the IL-4 levels of both adlay samples were lower than the control. These results strongly suggest that adlay leaves have anti-allergic effects similar to that seen with dehulled adlay seeds.

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Analyses of a mouse model of the dermatitis caused by 2,4,6-trinitro-1-chlorobenzene (TNCB)-repeated application

Cited by 30 publications

References 30 publications

Pharmacological Characterization of Itch-Associated Response Induced by Repeated Application of Oxazolone in Mice

Pharmacological Characterization of Itch-Associated Response Induced by Repeated Application of Oxazolone in Mice

Modification of the Picryl Chloride-Induced Allergic Dermatitis Model in Mouse Ear Lobes by 12-<i>O</i>-Tetradecanoylphorbol 13-Acetate, and Analysis of the Role of Histamine in the Modified Model

Effect of Administration of Adlay Leaves on 2,4,6-trinitro-1-chlorobenzene-induced Chronic Dermatitis in Mice

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