Analyse von stabil-isotop markierten Verbindungen

Schmidt, Hanns‐Ludwig

doi:10.1007/978-3-642-80804-3_8

Cited by 5 publications

(1 citation statement)

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“…Natural isotope fractionation effects could be separated into their intrinsic, physical effects and their additional components caused by environmental or biochemical factors, so that the latter could be filtered out. This progress made IR‐MS an important technique for areas such as climate research, ecology, archeology, food authentication, forensic sciences, and doping control, and IR‐MS developed a completely new application profile (for reviews see, e.g., Schmidt, ; Brenna, ; Brand & Coplen, ; Schmidt, ; Schmidt, Robins, & Werner, ). To account for the measurable natural variation of the atomic weight in some elements, the IUPAC Commission on Isotopic Abundances and Atomic Weights recently replaced their fixed “standard atomic weight” by an “atomic weight interval” (Wieser & Coplen, ; Coplen & Holden, ; Brand, ).…”

Section: –1980 Emergence Of Molecular Stable Isotope Dilution Witmentioning

confidence: 99%

A timeline of stable isotopes and mass spectrometry in the life sciences

Lehmann

2016

Mass Spec Rev

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This review retraces the role of stable isotopes and mass spectrometry in the life sciences. The timeline is divided into four segments covering the years 1920-1950, 1950-1980, 1980-2000, and 2000 until today. For each period methodic progress and typical applications are discussed. Application of stable isotopes is driven by improvements of mass spectrometry, chromatography, and related fields in sensitivity, mass accuracy, structural specificity, complex sample handling ability, data output, and data evaluation. We currently experience the vision of omics-type analyses, that is, the comprehensive identification and quantification of a complete compound class within one or a few analytical runs. This development is driven by stable isotopes without competition by radioisotopes. In metabolic studies as classic field of isotopic tracer experiments, stable isotopes and radioisotopes were competing solutions, with stable isotopes as the long-term junior partner. Since the 1990s the number of metabolic studies with radioisotopes decreases, whereas stable isotope studies retain their slow but stable upward tendency. Unique fields of stable isotopes are metabolic tests in newborns, metabolic experiments in healthy controls, newborn screening for inborn errors, quantification of drugs and drug metabolites in doping control, natural isotope fractionation in geology, ecology, food authentication, or doping control, and more recently the field of quantitative omics-type analyses. There, cells or whole organisms are systematically labeled with stable isotopes to study proteomic differences or specific responses to stimuli or genetic manipulation. The duo of stable isotopes and mass spectrometry will probably continue to grow in the life sciences, since it delivers reference-quality quantitative data with molecular specificity, often combined with informative isotope effects. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:58-85, 2017.

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