Analyse der Immunglobuline und Komplementfaktoren in Synovialflüssigkeit und Serum bei rheumatoider Arthritis, seronegativen Spondylarthropathien und Osteoarthritis: Pathophysiologie und retrospektive Analyse der klinischen Wertigkeit

Möller, Burkhard; Kessler, U.; Braner, Axel; Dielehner, N.; Kaltwasser, J. P.

doi:10.1007/s003930200006

Cited by 1 publication

(2 citation statements)

References 8 publications

(11 reference statements)

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“…C3 is a central protein involved in the classical pathway of complement activation [46]. Complement component proteins have been considered as potential diagnostic markers for SpAs, which is in keeping with our findings [43]. CD44, commonly known as hyaluronic acid receptor, is an inflammatory marker that has been studied in RA was found to be 2-fold upregulated in RA [47].…”

Section: Resultssupporting

confidence: 80%

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A multilectin affinity approach for comparative glycoprotein profiling of rheumatoid arthritis and spondyloarthropathy

et al. 2013

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BackgroundArthritis refers to inflammation of joints and includes common disorders such as rheumatoid arthritis (RA) and spondyloarthropathies (SpAs). These diseases differ mainly in terms of their clinical manifestations and the underlying pathogenesis. Glycoproteins in synovial fluid might reflect the disease activity status in the joints affected by arthritis; yet they have not been systematically studied previously. Although markers have been described for assisting in the diagnosis of RA, there are currently no known biomarkers for SpA.Materials and methodsWe sought to determine the relative abundance of glycoproteins in RA and SpA by lectin affinity chromatography coupled to iTRAQ labeling and LC-MS/MS analysis. We also used ELISA to validate the overexpression of VCAM-1, one of the candidate proteins identified in this study, in synovial fluid from RA patients.Results and discussionWe identified proteins that were previously reported to be overexpressed in RA including metalloproteinase inhibitor 1 (TIMP1), myeloperoxidase (MPO) and several S100 proteins. In addition, we discovered several novel candidates that were overexpressed in SpA including Apolipoproteins C-II and C-III and the SUN domain-containing protein 3 (SUN3). Novel molecules found overexpressed in RA included extracellular matrix protein 1 (ECM1) and lumican (LUM). We validated one of the candidate biomarkers, vascular cell adhesion molecule 1 (VCAM1), in 20 RA and SpA samples using ELISA and confirmed its overexpression in RA (p-value <0.01). Our quantitative glycoproteomic approach to study arthritic disorders should open up new avenues for additional proteomics-based discovery studies in rheumatological disorders.

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Section: Resultssupporting

confidence: 80%

“…This protein was identified to be 5-fold upregulated in SpA. C3 protein from synovial fluid of SpAs has been already observed to be higher than in RA [43]. It is also known to be elevated in ankylosing spondylitis and decreased in RA compared to unaffected joints [44,45].…”

Section: Resultsmentioning

confidence: 99%